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Detection of Puccinia kuehnii Causing Sugarcane Orange Rust with a Loop-Mediated Isothermal Amplification-Based Assay

Detection of Puccinia kuehnii Causing Sugarcane Orange Rust with a Loop-Mediated Isothermal Amplification-Based Assay | Rust fungi | Scoop.it
Puccinia kuehnii is a fungal pathogen that causes orange rust in sugarcane, which is now prevalent in many countries. At the early stage of disease, it is almost indistinguishable from brown rust, which is caused by Puccinia melanocephala. Although several PCR assays are available to detect these diseases, the loop-mediated isothermal amplification (LAMP)-based assay has been reported to be more economical and easier to perform. Under isothermal conditions, DNA is amplified with high specificity and rapidity. Moreover, visual judgment of color change without further post-amplification processing makes the method convenient. The present study was undertaken to detect P. kuehnii genomic DNA using four primers corresponding to a unique DNA sequence of P. kuehnii. The LAMP assay was found to be optimal when 8 mM MgSO4 was used and the reaction was incubated at 63 °C for 90 min. Positive samples showed a color change from orange to green upon SYBR Green I dye addition. Specificity of the LAMP test was checked with DNA of P. melanocephala, which showed no reaction. Sensitivity of the LAMP method was observed to be the same as real-time PCR at 0.1 ng, thus providing a rapid and more affordable option for early disease detection.


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Genome Biology and Evolution: Gene loss rather ...

Genome Biology and Evolution: Gene loss rather ... | Rust fungi | Scoop.it
Smut fungi are well-suited to investigate the ecology and evolution of plant pathogens, as they are strictly biotrophic, yet cultivable on media.
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Coffee fungus raising prices for high-end blends - WHLT22

Coffee fungus raising prices for high-end blends - WHLT22 | Rust fungi | Scoop.it
“Coffee fungus raising prices for high-end blends WHLT22 At issue is a fungus called coffee rust that has caused more than $1 billion in damage across Latin American region.”
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Microsatellite analysis of Icelandic populations of the poplar fungal pathogen Melampsora larici-populina shows evidence of repeated colonization events - Online First - Springer

Microsatellite analysis of Icelandic populations of the poplar fungal pathogen Melampsora larici-populina shows evidence of repeated colonization events - Online First - Springer | Rust fungi | Scoop.it
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Forests | Free Full-Text | Mapping Fusiform Rust Resistance Genes ...

Forests | Free Full-Text | Mapping Fusiform Rust Resistance Genes ... | Rust fungi | Scoop.it
“Here, we identify trees with Fr genes in a loblolly pine population derived from a complex mating design challenged with two Cqf inocula (one gall and 10 gall mixtures). We used single nucleotide polymorphism (SNP) ...”
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The genome sequence and effector complement of ...

The genome sequence and effector complement of ... | Rust fungi | Scoop.it
“Rust fungi cause serious yield reductions on crops, including wheat, barley, soybean, coffee, and represent real threats to global food security.”
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Frontiers | Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat | Frontiers in Plant-Microbe Interaction

Frontiers | Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat | Frontiers in Plant-Microbe Interaction | Rust fungi | Scoop.it
To understand gene expression during infection and find genetic differences associated with races, RNA from wheat leaves infected with six different rust races, at 6 days post inoculation, was sequenced using Illumina. As P. triticina is an obligate biotroph, RNA from both the host and fungi were present and separated by alignment to the P. triticina genome and a wheat EST reference. A total of 222,571 rust contigs were assembled from 165 million reads. An examination of the resulting contigs revealed 532 predicted secreted proteins among the transcripts. Of these, 456 were found in all races.
Via Bradford Condon
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PLOS Pathogens: Origin, Migration Routes and Worldwide Population Genetic Structure of the Wheat Yellow Rust Pathogen Puccinia striiformis f.sp. tritici

PLOS Pathogens: Origin, Migration Routes and Worldwide Population Genetic Structure of the Wheat Yellow Rust Pathogen Puccinia striiformis f.sp. tritici | Rust fungi | Scoop.it
From molecules to physiology
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Walk in the woods inspires work on cranberry rust leads B.C. teen to Taiwan for international science fair

Walk in the woods inspires work on cranberry rust leads B.C. teen to Taiwan for international science fair | Rust fungi | Scoop.it
It was just a routine walk among wild cranberry bushes in northwest B.C., but the results of the trek are taking 16-year-old Kiri Daust all the way to an international science fair in Taiwan.
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Frontiers | Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat. | Frontiers in Plant-Microbe Interaction

“ Wheat leaf rust, caused by the basidiomycete Puccinia triticina, can cause yield losses of up to 20% in wheat producing regions.”
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Scavenging Fungi Is A Plant's Best Friend - RedOrbit

Scavenging Fungi Is A Plant's Best Friend - RedOrbit | Rust fungi | Scoop.it
“RedOrbit Scavenging Fungi Is A Plant's Best Friend RedOrbit Rhizophagus irregularis, is the next in this linage to be released by the DOE JGI, it follows the ectomycorrhizal fungal symbiont Laccaria, the poplar rust pathogen Melampsora, and dozens...”
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Rust disease of eucalypts, caused by Puccinia psidii, did not originate via host jump from guava in Brazil - Graça - 2013 - Molecular Ecology - Wiley Online Library

Rust disease of eucalypts, caused by Puccinia psidii, did not originate via host jump from guava in Brazil - Graça - 2013 - Molecular Ecology - Wiley Online Library | Rust fungi | Scoop.it
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Comparative Genomics of Plant Fungal Pathogens: The Ustilago-Sporisorium Paradigm

Comparative Genomics of Plant Fungal Pathogens: The Ustilago-Sporisorium Paradigm | Rust fungi | Scoop.it
From molecules to physiology
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Genetic evidence for three discrete taxa of Melampsora (Pucciniales) affecting willows (Salix spp.) in New York State

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Steve Marek's curator insight, May 30, 2014 10:02 AM

This seems to be a common theme with rusts

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Fitness dynamics within a poplar hybrid zone: I. Prezygotic and postzygotic barriers impacting a native poplar hybrid stand - Roe - 2014 - Ecology and Evolution - Wiley Online Library

Fitness dynamics within a poplar hybrid zone: I. Prezygotic and postzygotic barriers impacting a native poplar hybrid stand - Roe - 2014 - Ecology and Evolution - Wiley Online Library | Rust fungi | Scoop.it
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A reassessment of the risk of rust fungi developing resistance to fungicides. - Oliver - Pest Management Science - Wiley Online Library

A reassessment of the risk of rust fungi developing resistance to fungicides. - Oliver - Pest Management Science - Wiley Online Library | Rust fungi | Scoop.it
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White Pine Blister Rust and its threat to to High Elevation White Pines

White Pine Blister Rust and its threat to to High Elevation White Pines | Rust fungi | Scoop.it
“Learn about bristlecone, foxtail, limber and whitebark pines, their ecosystems and the threats that face them. (RT @m_m_campbell: Rust never sleeps. Despite durability, bristlecone pine threatened by rust fungus.”
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How acne hopped from humans to grapevines

How acne hopped from humans to grapevines | Rust fungi | Scoop.it
“In the first instance of a human-to-plant pathogen transfer, a bacterium related to the one that causes pimples has been found on a common grapevine.”
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Sr31-Virulent Races (TTKSK, TTKST, and TTTSK) of the Wheat Stem Rust Pathogen Puccinia graminis f. sp. tritici are Present in Tanzania

Sr31-Virulent Races (TTKSK, TTKST, and TTTSK) of the Wheat Stem Rust Pathogen Puccinia graminis f. sp. tritici are Present in Tanzania | Rust fungi | Scoop.it
Since the first detection of race TTKSK (syn. Ug99) in Uganda in 1999 (2), the migration and evolution of Sr31-virulent races ofPuccinia graminis f. sp. tritici [Pgt] have been closely monitored, particularly in Kenya and countries north, along the likely trajectory of migration to major wheat-producing regions of Asia. More recently, surveillance efforts have been undertaken to the south as well, and Ug99-related races have been detected in South Africa and Zimbabwe (3,4). Here we report for the first time results of a survey conducted in Tanzania. Systematic race surveillance provides data not only on the current distribution of the Ug99 race group, but also on the possible points of origin as well as the pace and probable paths of dispersal of future races from the region. In this context, the presence or absence of the Ug99 group of wheat stem rust races in adjacent countries like Tanzania assumes regional, and possibly global, relevance. A preliminary survey conducted in September 2006 indicated the presence of Sr31-virulent races of Pgt outside Slahhamo Village (3°15′S, 35°48′E) in the Ngorongoro highlands of northern Tanzania, based on compatible reactions with cv. K-Mamba (a.k.a. Mwamba), a cultivar whose pedigree indicates the presence ofSr31. A broader survey was conducted in August 2009, during which infected tissue was collected from currently-grown cultivars in research plots and on large estates, as well as from the mixes of older cultivars common on smallholder farms. In all, Pgt-infected samples were collected from one site in the Arusha region [Monduli (3°16′ S, 36°24′E)], three sites in the Ngorongoro highlands [Karatu (3°20′ S, 35°40′ E), Upper Kitete (3°14′ S, 35°53′ E), and Slahhamo], one site in the Manyara region [Hanang (4°43′ S, 35°40′ E)], and one site in the Mbeya region [southern highlands (8°87′ S, 33°40′ E)], thereby giving representation to all four major wheat growing areas in the country. Sample storage, inoculation, incubation, disease assessment, and derivation of single-pustule cultures were all performed according to the methods described by Jin et al. (1). In addition to the 20 differentials in the expanded Pgt differential set of North America, we included two supplemental tester lines: Siouxland (Sr24 + Sr31) and Sisson (Sr31 + Sr36). Each single-pustule-derived isolate was evaluated for virulence on the differential and supplemental lines at least twice. A total of 39 single-pustule isolates were derived from the six collection sites. All 39 isolates were identified as belonging to the Ug99 race group, with six identified as TTKSK (all four regions), 30 identified as TTKST (Sr31 + Sr24 virulence; Arusha region and the Ngorongoro highlands), and three identified as TTTSK (Sr31 + Sr36 virulence; Manyara region and the Ngorongoro highlands). The results of this study suggest that, to more precisely locate the “hot spots” and thereby gain a better understanding of the mechanisms of novel race emergence in East Africa, it would be prudent to include Tanzania, heretofore a blank area on the wheat rust surveillance map, in future systematic race monitoring efforts.
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Genetics of Five-Needle Pines and Rusts in Mountain Landscapes ...

Genetics of Five-Needle Pines and Rusts in Mountain Landscapes ... | Rust fungi | Scoop.it
“Joint Meeting Breeding and Genetic Resources of Five-Needle Pines (IUFRO 2.02.15) Rusts of Forest Trees (IUFRO 7.02.05) Strobusphere The joint meeting will be held June 15-20, 2014 in Ft. Collins, Colorado, U.S.A..”
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Genome sequencing provides insight into the reproductive biology, nutritional mode and ploidy of the fern pathogen Mixia osmundae - Toome - 2013 - New Phytologist - Wiley Online Library

Genome sequencing provides insight into the reproductive biology, nutritional mode and ploidy of the fern pathogen Mixia osmundae - Toome - 2013 - New Phytologist - Wiley Online Library | Rust fungi | Scoop.it
“Mixia osmundae, an unusual fern pathogen (Pucciniomycotina), has the smallest plant pathogenic basidiomycete genome. http://t.co/OPUF67dUz1”;
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Steve Marek's curator insight, January 3, 2014 3:18 PM

Mixia, culturable rust relative

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Researchers backed to map Ug99-resistant genes - AGCanada

Researchers backed to map Ug99-resistant genes - AGCanada | Rust fungi | Scoop.it
“Should (Ug99) become established in Canada it would devastate the Canadian cereals sector, a situation that had serious economic consequences for Canada during previous cereal rust outbreaks in the last century,” AAFC ...
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Resource: Wheat Yellow Rust Genomics data now available (2013)

Resource: Wheat Yellow Rust Genomics data now available (2013) | Rust fungi | Scoop.it
We have been working with colleagues in India, Kenya, Ethiopia, Denmark and the UK to develop wheat yellow rust genomics through the re-sequencing of isolates from different continents. Our project also includes traditional field trials of a common set of germplasm which has been evaluated in these countries. One of our goals is to make the genomic data of wheat yellow rust isolates and phenotypic data (Yr, Sr, Lr) from the wheat Watkins landraces rapidly and broadly available to the scientific community, as stated at the BGRI workshop 2013. We hope that this data will be of use to others in the community and we have made this information available (open access) through our project website (www.yellowrust.com). It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analyses. We strongly believe that as a global research community we can have a greater impact in our common goals if data is shared openly and used responsibly. Diane Saunders and Cristobal Uauy on behalf of SCPRID project partners
Via Kamoun Lab @ TSL
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