Reconstructive Surgery
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Reconstructive Surgery
Interesting news, cases and dossiers about reconstructive surgery.
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TALEN-mediated gene editing of the thrombospondin-1 locus in axolotl - Regeneration

TALEN-mediated gene editing of the thrombospondin-1 locus in axolotl - Regeneration | Reconstructive Surgery | Scoop.it

Kuo et al, 2015

Loss-of-function genetics provides strong evidence for a gene's function in a wild-type context. In many model systems, this approach has been invaluable for discovering the function of genes in diverse biological processes. Axolotls are urodele amphibians (salamanders) with astonishing regenerative abilities, capable of regenerating entire limbs, portions of the tail (including spinal cord), heart, and brain into adulthood. With their relatively short generation time among salamanders, they offer an outstanding opportunity to interrogate natural mechanisms for appendage and organ regeneration provided that the tools are developed to address these long-standing questions. Here we demonstrate targeted modification of the thrombospondin-1 locus using transcription activator like effector nucleases (TALENs) and identify a role of thrombospondin-1(tsp-1) in recruitment of myeloid cells during limb regeneration. We find that while tsp-1-edited mosaic animals still regenerate limbs, they exhibit a reduced subepidermal collagen layer in limbs and an increased number of myeloid cells within blastemas. This work presents a protocol for generating and genotyping mosaic axolotls with TALEN-mediated gene


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The Journal of Craniofacial Surgery Honors Dr. Joseph E. Murray

The field of surgery lost a giant last year with the death of Joseph E. Murray, MD, Nobel laureate in medicine and biology and a pioneer in both transplantation and craniofacial surgery. A special tribute to Dr.
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At a time when transplantation surgery is a specialty in its own right, it may be surprising to learn that Dr Murray's specialty was plastic surgery. His interest in transplantation developed from his experience with skin grafting as a military surgeon treating seriously burned patients.

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