Quantitative PCR for gene expression analyses
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Quantitative PCR for gene expression analyses
Real-time PCR is a preferred and precise method for analyzing gene expression, whether you use 5′ nuclease (probe-based) assays or intercalating dye-based assays.
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IDT Presents Data on RNase H2-Dependent PCR Genotyping Products at AACR

To address the broader needs of genomics researchers, Integrated DNA Technologies has begun offering one of its core technologies as a standalone product. The firm presented performance data for its RNase H2-based multiplex qPCR technology this week at the American Association of Cancer Research meeting in Washington, DC.

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Improving gene expression experiments with PrimeTime® Gene Expression Master Mix

Improving gene expression experiments with PrimeTime® Gene Expression Master Mix | Quantitative PCR for gene expression analyses | Scoop.it

Performance data and experimental advice for better qPCR research

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Dengue, Zika transmission slowed by Wolbachia bacterium

Dengue, Zika transmission slowed by Wolbachia bacterium | Quantitative PCR for gene expression analyses | Scoop.it

With ZEN™ Double-Quenched Probes, the Eliminate Dengue Program in Brazil has successfully applied bacteria-based biocontrol method to Zika virus

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A qPCR master mix stable enough for ambient shipping

A qPCR master mix stable enough for ambient shipping | Quantitative PCR for gene expression analyses | Scoop.it

Choose a qPCR master mix shipped at ambient temperature. Why? PrimeTime® Gene Expression Master Mix consistently provides high qPCR efficiency, and ambient shipping directly benefits you by reducing shipping costs and time. Ambient shipping is also environmentally friendlier, as fewer resources are required to make and dispose of dry or gel ice packaging materials. Learn more about the strong thermal stability of this master mix.

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Development of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup II

Development of single-tube nested real-time PCR assays with long internally quenched probes for detection of norovirus genogroup II | Quantitative PCR for gene expression analyses | Scoop.it

The high sequence variation of RNA viruses necessitates use of degenerate primers and probes or multiple primers and probes in molecular diagnostic assays. We showed previously that PCR amplification in two rounds, first with long target-specific primers and then with short generic primers, followed by detection using long probes, can tolerate sequence variation. Here we demonstrate that long primers and probes of up to 56 nucleotides can also be applied in real-time PCR for the detection of norovirus genogroup II with improved sensitivity. Probe design (method of incorporating quenchers, use of Zen internal quencher or traditional quenchers) greatly affects the sensitivity of the real-time PCR assays.

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IDT delivers a complete solution for probe-based qPCR Assays

IDT delivers a complete solution for probe-based qPCR Assays | Quantitative PCR for gene expression analyses | Scoop.it

PrimeTime® qPCR Assays and Master Mix from IDT provide superior efficiencies and reliability for gene expression studies

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BGU and MIT Researchers Develop Rapid Method for Water, Air and Soil Pathogen Screening

BGU and MIT Researchers Develop Rapid Method for Water, Air and Soil Pathogen Screening | Quantitative PCR for gene expression analyses | Scoop.it

Researchers at BGU and the Massachusetts Institute of Technology (MIT) have developed a highly sensitive, cost-effective technology for rapid bacterial pathogen screening of air, soil, water, and agricultural produce in as little as 24 hours. 

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High throughput qPCR: tips for analysis across multiple plates

Real-time quantitative PCR (qPCR) is a preferred platform for high throughput gene expression profiling, where large numbers of samples are characterized for hundreds of expression markers. Technically, the qPCR measurements are performed in the same way as when classical qPCR is used to analyze only a few targets per sample, but the higher throughput introduces additional sources of potential confounding variation that must be controlled for. In this presentation, Dr Kubista describes how high throughput qPCR profiling studies are designed. He covers assay optimization and validation, sample quality testing, and how to merge multi-plate measurements into a common analysis. Dr Kubista also discusses how to cost-effectively measure and compensate for background due to genomic DNA.

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Webinar: High throughput qPCR: tips for analysis across multiple plates

Webinar: High throughput qPCR: tips for analysis across multiple plates | Quantitative PCR for gene expression analyses | Scoop.it

Real-time quantitative PCR (qPCR) is a preferred platform for high throughput gene expression profiling, where large numbers of samples are characterized for hundreds of expression markers. Technically, the qPCR measurements are performed in the same way as when classical qPCR is used to analyze only a few targets per sample, but the higher throughput introduces additional sources of potential confounding variation that must be controlled for. In this presentation, Dr Kubista will describe how high throughput qPCR profiling studies are designed. He will also cover assay optimization and validation, sample quality testing, and how to merge multi-plate measurements into a common analysis. Dr Kubista will also talk about how to cost-effectively measure and compensate for background due to genomic DNA.

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Increase sensitivity and precision in your qPCR experiments

Increase sensitivity and precision in your qPCR experiments | Quantitative PCR for gene expression analyses | Scoop.it

Learn how you can use double-quenched probes to decrease background, and increase sensitivity and precision in your qPCR experiments.

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Developing a qPCR point-of-care diagnostic for Ebola

Developing a qPCR point-of-care diagnostic for Ebola | Quantitative PCR for gene expression analyses | Scoop.it

Learn how Ubiquitome, Battelle, and IDT are developing a rapid qPCR Ebola virus test for easy use in the field. The PrimeTime qPCR assay is designed to be run on Ubiquitome’s hand-held, battery powered real-time PCR device, the Freedom4.

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Shedding of Ebola Virus in an Asymptomatic Pregnant Woman

In West Africa, the worst outbreak of Ebola virus disease (EVD) in history is continuing, with more than 11,100 deaths caused by the Zaire species (Zaire ebolavirus [EBOV]).1 Symptoms include fever, headache, body pain, nausea with vomiting, diarrhea, hemorrhage, and symptoms of septic shock and multiorgan failure. It is thought that transmission occurs only through contact with body fluids from symptomatic patients.2

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Diagnostic tests plumb depths of ‘hidden malaria’

Diagnostic tests plumb depths of ‘hidden malaria’ | Quantitative PCR for gene expression analyses | Scoop.it

A self-styled ‘ultrasensitive’ malaria test could lead to more accurate identification of the potentially significant pool of people who carry the disease without showing any symptoms, a paper has found.

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Considering SNPs when designing PCR and qPCR assays

Considering SNPs when designing PCR and qPCR assays | Quantitative PCR for gene expression analyses | Scoop.it

The rapidly increasing number of known SNPs

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High efficiency qPCR with PrimeTime® Gene Expression Master Mix from IDT

Real-Time quantitative PCR (qPCR) is a mainstream method that is used in research and diagnostic applications for quantification of gene expression. IDT has developed a robust and affordable qPCR master mix for use with probe-based qPCR in single and multiplex assays. In this presentation, we explore a variety of applications of PrimeTime® Gene Expression Master Mix. We cover the use of PrimeTime master mix with probe based assays from IDT. We also look at the use of PrimeTime master mix in multiplex applications without the loss of sensitivity that is commonly observed. Finally, we demonstrate the unmatched stability of PrimeTime master mix under ambient temperatures, saving your research money and minimizing on shipping delays.

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Use splice junctions to your advantage in qPCR

Use splice junctions to your advantage in qPCR | Quantitative PCR for gene expression analyses | Scoop.it

Get recommendations for avoiding PCR amplification of genomic DNA, as well as for identifying and quantifying splice variants, in this article on designing qPCR assays that span splice junctions.

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qPCR assays for optimized viral detection in clinical samples

qPCR assays for optimized viral detection in clinical samples | Quantitative PCR for gene expression analyses | Scoop.it

Learn how ZEN™ Double-Quenched Probes were used in a unique qPCR experiment to help rapidly and accurately detect the highly variable norovirus in clinical samples.

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Improved pathogen detection by multiplex RT-qPCR

Improved pathogen detection by multiplex RT-qPCR | Quantitative PCR for gene expression analyses | Scoop.it

Learn how gBlocks Gene Fragments can help optimize multiplex qPCR reactions for pathogen detection in human clinical samples.

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Obtain high efficiency qPCR results using PrimeTime® Gene Expression Master Mix

Obtain high efficiency qPCR results using PrimeTime® Gene Expression Master Mix | Quantitative PCR for gene expression analyses | Scoop.it

Product Spotlight: If you are doing 5′ nuclease assays for qPCR or 2-step qPCR, we have a reliable master mix for you to use in singleplex, multiplex, or high-throughput applications.

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Choosing a qPCR assay: Inventoried or predesigned?

Choosing a qPCR assay: Inventoried or predesigned? | Quantitative PCR for gene expression analyses | Scoop.it

You may know that several companies offer inventoried qPCR assays. But did you know that these assays are often pre-manufactured and stocked, waiting perhaps for years for that order to be placed? In contrast, IDT PrimeTime® Predesigned qPCR Assays are manufactured at the time of order, and can therefore take into account sequence updates such as new SNPs.

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Device Could Speed Diagnosis of Infections

Device Could Speed Diagnosis of Infections | Quantitative PCR for gene expression analyses | Scoop.it

A new device created by a collaborative team of UA engineers and scientists may significantly reduce the amount of time necessary to diagnose tissue infections.

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Optimizing multiplex qPCR for detecting infectious diseases and biothreat agents in the field

Optimizing multiplex qPCR for detecting infectious diseases and biothreat agents in the field | Quantitative PCR for gene expression analyses | Scoop.it

Researchers at Tetracore specialize in developing large sets of robust probe-based qPCR assays for use in a multiplex format to detect infectious diseases and bio-terrorism threat agents. Here they discuss the need to: use probe dyes compatible on common PCR instruments, maintain low background with multiple probes, and reformulate assays to address viral mutation; and how ZEN™ Double-Quenched Probes have helped meet these criteria.

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Melt-Curve analysis for improved intercalating dye qPCR

Melt-Curve analysis for improved intercalating dye qPCR | Quantitative PCR for gene expression analyses | Scoop.it

Performing qPCR using intercalating dyes, such as SYBR® dyes, involves use of just 2 amplification primers to provide assay specificity. Because fluorescence in intercalating dye assays is based on the production of any double-stranded DNA, off-target amplification can generate false-positive signals, compromising assay data. To address this problem, researchers use melt-curve analysis to assess whether their assays have produced single, specific products. 

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MGB Eclipse Probes for human in vitro diagnostics

MGB Eclipse Probes for human in vitro diagnostics | Quantitative PCR for gene expression analyses | Scoop.it

Obtain MGB Eclipse® Probes for human in vitro diagnostic end-use applications. Selecting MGB Eclipse® Probes made by the IDT GMP manufacturing division provides you with complete process transparency and product traceability, in addition to consistent, reliable oligonucleotide quality.

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The Lab Side: Developing qPCR Assays

The Lab Side: Developing qPCR Assays | Quantitative PCR for gene expression analyses | Scoop.it

Michelle and I have spent most of April and June in the Becker lab preparing polymerase chain reactions for each of the three species we are sampling this summer (Pacific geoduck clam, Pacific oyster, Manila clam). This is an extensive, but necessary process that needs to be done as a foundation for the more complex real-time quantitative PCR, or qPCR for short.

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