The normal procedure would be to culture the pathogen and sequence the genome and transcriptome from cultured material and controlled laboratory infections. Here, we decided to take the unusual step of directly sequencing the “interaction transcriptome” of a lesion dissected from an infected ash twig. This was the most rapid way to proceed to generate useful information without proceeding through standard laboratory culturing. This is the shortest route from the wood to the sequencer to the computer. The question that many of you must be asking is how useful is this data? This post addresses this question and summarizes the preliminary analyses that the TSL team has produced.