Proteomics and other sciency stuff
544 views | +4 today
Follow
Your new post is loading...
Your new post is loading...
Scooped by sjossie
Scoop.it!

Progress and pitfalls in finding the ‘missing proteins’ from the human proteome map

Progress and pitfalls in finding the ‘missing proteins’ from the human proteome map | Proteomics and other sciency stuff | Scoop.it
(2016). Progress and pitfalls in finding the ‘missing proteins’ from the human proteome map. Expert Review of Proteomics. Ahead of Print. doi: 10.1080/14789450.2017.1265450
more...
No comment yet.
Scooped by sjossie
Scoop.it!

LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics. - PubMed - NCBI

LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics. - PubMed - NCBI | Proteomics and other sciency stuff | Scoop.it
Methods Mol Biol. 2017;1546:57-73.
more...
No comment yet.
Scooped by sjossie
Scoop.it!

An application of mass spectrometry for quality control of biologicals: highly sensitive profiling of plasma residuals in human plasma-derived immunoglobulin

An application of mass spectrometry for quality control of biologicals: highly sensitive profiling of plasma residuals in human plasma-derived immunoglobulin | Proteomics and other sciency stuff | Scoop.it
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Versatile, sensitive liquid chromatography mass spectrometry – Implementation of 10 μm OT columns suitable for small molecules, peptides and proteins

Versatile, sensitive liquid chromatography mass spectrometry – Implementation of 10 μm OT columns suitable for small molecules, peptides and proteins | Proteomics and other sciency stuff | Scoop.it
We have designed a versatile and sensitive liquid chromatographic (LC) system, featuring a monolithic trap column and a very narrow (10 μm ID) fused silica open tubular liquid chromatography (OTLC) separation column functionalized with C18-groups, for separating a wide range of molecules (from small metabolites to intact proteins).
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Need the perfect standard for intact MS and/or top-down? Check this out!

Need the perfect standard for intact MS and/or top-down? Check this out! | Proteomics and other sciency stuff | Scoop.it
Shoutout to Bob for tipping me off to this one! Is this the perfect mixture for optimizing your intact protein mass measurement and/o
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Immuno-affinity Capture Followed by TMPP N-Terminus Tagging to Study Catabolism of Therapeutic Proteins - Journal of Proteome Research (ACS Publications)

Immuno-affinity Capture Followed by TMPP N-Terminus Tagging to Study Catabolism of Therapeutic Proteins - Journal of Proteome Research (ACS Publications) | Proteomics and other sciency stuff | Scoop.it
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Automation, Microsampling Key to Clinical Adoption of Mass Spec-Based Proteomics, Researcher Says

Automation, Microsampling Key to Clinical Adoption of Mass Spec-Based Proteomics, Researcher Says | Proteomics and other sciency stuff | Scoop.it
According to Cedars Sinai Medical Center's Jennifer Van Eyk, mass spec-based proteomics' clinical potential is on the cusp of being realized.
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Automatic and rapid identification of glycopeptides by nano-UPLC-LTQ-FT-MS and proteomic search engine

Automatic and rapid identification of glycopeptides by nano-UPLC-LTQ-FT-MS and proteomic search engine | Proteomics and other sciency stuff | Scoop.it
more...
No comment yet.
Scooped by sjossie
Scoop.it!

moFF: a robust and automated approach to extract peptide ion intensities : Nature Methods : Nature Research

moFF: a robust and automated approach to extract peptide ion intensities : Nature Methods : Nature Research | Proteomics and other sciency stuff | Scoop.it
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Single Amino Acid Repeats in the Proteome World: Structural, Functional, and Evolutionary Insights

Single Amino Acid Repeats in the Proteome World: Structural, Functional, and Evolutionary Insights | Proteomics and other sciency stuff | Scoop.it
Microsatellites or simple sequence repeats (SSR) are abundant, highly diverse stretches of short DNA repeats present in all genomes. Tandem mono/tri/hexanucleotide repeats in the coding regions contribute to single amino acids repeats (SAARs) in the proteome. While SSRs in the coding region always result in amino acid repeats, a majority of SAARs arise due to a combination of various codons representing the same amino acid and not as a consequence of SSR events. Certain amino acids are abundant in repeat regions indicating a positive selection pressure behind the accumulation of SAARs. By analysing 22 proteomes including the human proteome, we explored the functional and structural relationship of amino acid repeats in an evolutionary context. Only ~15% of repeats are present in any known functional domain, while ~74% of repeats are present in the disordered regions, suggesting that SAARs add to the functionality of proteins by providing flexibility, stability and act as linker elements between domains. Comparison of SAAR containing proteins across species reveals that while shorter repeats are conserved among orthologs, proteins with longer repeats, >15 amino acids, are unique to the respective organism. Lysine repeats are well conserved among orthologs with respect to their length and number of occurrences in a protein. Other amino acids such as glutamic acid, proline, serine and alanine repeats are generally conserved among the orthologs with varying repeat lengths. These findings suggest that SAARs have accumulated in the proteome under positive selection pressure and that they provide flexibility for optimal folding of functional/structural domains of proteins. The insights gained from our observations can help in effective designing and engineering of proteins with novel features.
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Glycan analysis of protists! And other cool new Springer Protocols

Glycan analysis of protists! And other cool new Springer Protocols | Proteomics and other sciency stuff | Scoop.it
Honestly, just leaving this here to remind myself to grab this new book at the library! But, how awesome does this new protocols boo
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Advancing Top-down Analysis of the Human Proteome Using a Benchtop Quadrupole-Orbitrap Mass Spectrometer - Journal of Proteome Research (ACS Publications)

Advancing Top-down Analysis of the Human Proteome Using a Benchtop Quadrupole-Orbitrap Mass Spectrometer - Journal of Proteome Research (ACS Publications) | Proteomics and other sciency stuff | Scoop.it
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Mass Spectrometry Adopted by Clinical Laboratories Around the World

Mass Spectrometry Adopted by Clinical Laboratories Around the World | Proteomics and other sciency stuff | Scoop.it
NEW YORK, December 2, 2016 /PRNewswire/ -- Mass Spectrometry Adopted by Clinical Laboratories Around the World.
more...
No comment yet.
Scooped by sjossie
Scoop.it!

iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification. - PubMed - NCBI

iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification. - PubMed - NCBI | Proteomics and other sciency stuff | Scoop.it
Methods Mol Biol. 2017;1546:267-274.
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Inference Method for Developing Mathematical Models of Cell Signaling Pathways Using Proteomic Datasets - Springer

Inference Method for Developing Mathematical Models of Cell Signaling Pathways Using Proteomic Datasets - Springer | Proteomics and other sciency stuff | Scoop.it
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Using 15N-Metabolic Labeling for Quantitative Proteomic Analyses. - PubMed - NCBI

Using 15N-Metabolic Labeling for Quantitative Proteomic Analyses. - PubMed - NCBI | Proteomics and other sciency stuff | Scoop.it
Methods Mol Biol. 2017;1546:235-243.
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Thermo MSF Parser!

Thermo MSF Parser! | Proteomics and other sciency stuff | Scoop.it
Wow! Am I missing more stuff all the time? Starting to feel like an NBA referee. (The Will Smith video above is hilarious, btw).
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Intomics Opening US Office, Launching Standalone Version of Protein Interaction Tool

Intomics Opening US Office, Launching Standalone Version of Protein Interaction Tool | Proteomics and other sciency stuff | Scoop.it
The Copenhagen-based firm published the first public demonstration of its InBio Map protein interaction tool in a study released this week in Nature Methods.
more...
No comment yet.
Scooped by sjossie
Scoop.it!

The unbalanced distribution of the amino aicds(Arg, Lys) in high pI proteins of complete proteomes, its origin and evolution

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Proteomics: Proteome quantification compared : Nature Methods : Nature Research

Careful benchmarking improves software methods for analyzing data-independent-acquisition mass spectrometry data.
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Proteomics: Proteome quantification compared : Nature Methods : Nature Research

Careful benchmarking improves software methods for analyzing data-independent-acquisition mass spectrometry data.
more...
No comment yet.
Scooped by sjossie
Scoop.it!

Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers

Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers | Proteomics and other sciency stuff | Scoop.it
Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers
more...
No comment yet.