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Proteomic Applications in Microbial Taxonomy
MALDI-ToF MS application for microbial identification and characterization.
Curated by Praveen Rahi
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Use of MALDI-TOF mass spectrometry fingerprinting to characterize Enterococcus spp. and Escherichia coli isolates

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Mass spectrometry and tandem mass spectrometry characterization of protein patterns, protein markers and whole proteomes for pathogenic bacteria

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Mass spectrometry and tandem mass spectrometry will be the techniques for future microbial taxonomy

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MALDI-TOF Identification of the Human Gut Microbiome in People with and without Diarrhea in Senegal

MALDI-TOF Identification of the Human Gut Microbiome in People with and without Diarrhea in Senegal | Proteomic Applications in Microbial Taxonomy | Scoop.it
PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
Praveen Rahi's insight:

a quick identification of bacterial species

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PLOS Neglected Tropical Diseases: Identification of Tsetse (Glossina spp.) Using Matrix-Assisted Laser Desorption/Ionisation Time of Flight Mass Spectrometry

PLOS Neglected Tropical Diseases: Identification of Tsetse (Glossina spp.) Using Matrix-Assisted Laser Desorption/Ionisation Time of Flight Mass Spectrometry | Proteomic Applications in Microbial Taxonomy | Scoop.it
PLOS Neglected Tropical Diseases is an open-access journal publishing peer-reviewed research on the world\'s most neglected tropical diseases, such as elephantiasis, river blindness, leprosy, hookworm, schistosomiasis, and African sleeping sickness...
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Severe Wound Infection with Photobacterium damselae ssp ...

Severe Wound Infection with Photobacterium damselae ssp ... | Proteomic Applications in Microbial Taxonomy | Scoop.it
Recent developments in medical microbiology, in particular the Matrix-Assisted Laser Desorption-Ionization Time of Flight Mass Spectrometry (MALDI-TOF) technology [1], enable fast and accurate identifications of bacteria, even of taxa, which are...
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Rescooped by Praveen Rahi from Rhizobium Research
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Legume growth-promoting rhizobia: An overview on the Mesorhizobium genus

The need for sustainable agricultural practices is revitalizing the interest in biological nitrogen fixation and rhizobia-legumes symbioses, particularly those involving economically important legume crops in terms of food and forage. The genus Mesorhizobium includes species with high geographical dispersion and able to nodulate a wide variety of legumes, including important crop species, like chickpea or biserrula. Some cases of legume-mesorhizobia inoculant introduction represent exceptional opportunities to study the rhizobia genomes evolution and the evolutionary relationships among species. Complete genome sequences revealed that mesorhizobia typically harbour chromosomal symbiosisislands. The phylogenies of symbiosis genes, such as nodC, are not congruent with the phylogenies based on core genes, reflecting rhizobial host range, rather than species affiliation. This agrees with studies showing that Mesorhizobium species are able to exchange symbiosis genes through lateral transfer of chromosomal symbiosis islands, thus acquiring the ability to nodulate new hosts. Phylogenetic analyses of the Mesorhizobium genus based on core and accessory genes reveal complex evolutionary relationships and a high genomic plasticity, rendering the Mesorhizobium genus as a good model to investigate rhizobia genome evolution and adaptation to different host plants. Further investigation ofsymbiosis genes as well as stress response genes will certainly contribute to understand mesorhizobia-legume symbiosis and to develop more effective mesorhizobia inoculants.

 

Laranjo M, Alexandre A, Oliveira S. (2013). Microbiol Res. Sep 27.  [Epub ahead of print]


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How to make the highest accuracy Microbial Stool Analysis

How to make the highest accuracy Microbial Stool Analysis | Proteomic Applications in Microbial Taxonomy | Scoop.it
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Subtype determination of Blastocystis isolates by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) - Online First - Springer

Subtype determination of Blastocystis isolates by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) - Online First - Springer | Proteomic Applications in Microbial Taxonomy | Scoop.it
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Accurate and universal delineation of prokaryotic species


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Dmitry Alexeev's curator insight, August 6, 2013 2:50 AM

The exponentially increasing number of sequenced genomes necessitates fast, accurate, universally applicable and automated approaches for the delineation of prokaryotic species. We developed specI (species identification tool;http://www.bork.embl.de/software/specI/), a method to group organisms into species clusters based on 40 universal, single-copy phylogenetic marker genes. Applied to 3,496 prokaryotic genomes, specI identified 1,753 species clusters. Of 314 discrepancies with a widely used taxonomic classification, >62% were resolved by literature support.

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Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology


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Praveen Rahi's comment, July 4, 2013 11:19 PM
welcome :-)
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Characterisation of different food-isolated Enterococcus strains by MALDI-TOF mass fingerprinting - Quintela-Baluja - ELECTROPHORESIS - Wiley Online Library

Characterisation of different food-isolated Enterococcus strains by MALDI-TOF mass fingerprinting - Quintela-Baluja - ELECTROPHORESIS - Wiley Online Library | Proteomic Applications in Microbial Taxonomy | Scoop.it
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Rapid Identification Of Mycobacterium Tuberculosis No Dream India Biology Essay

Rapid Identification Of Mycobacterium Tuberculosis No Dream India Biology Essay | Proteomic Applications in Microbial Taxonomy | Scoop.it
Among the infectious diseases that are prevalent throughout the developing world, Tuberculosis (TB) continues to be a major public health issue...
Praveen Rahi's insight:

MALDI-TOF MS making microbial identification fast, easy and reliable.

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PLOS ONE: MALDI-TOF MS Distinctly Differentiates Nontypable Haemophilus influenzae from Haemophilus haemolyticus

PLOS ONE: MALDI-TOF MS Distinctly Differentiates Nontypable Haemophilus influenzae from Haemophilus haemolyticus | Proteomic Applications in Microbial Taxonomy | Scoop.it
PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
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Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS

Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS | Proteomic Applications in Microbial Taxonomy | Scoop.it
PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
Praveen Rahi's insight:

Unique protein profile and chemometric analysis are valuable tools for B. pumilus and B. safensis discrimination

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Rapid Identification of Gram Negative Bacteria from... | Protocol - JoVE

Rapid Identification of Gram Negative Bacteria from... | Protocol - JoVE | Proteomic Applications in Microbial Taxonomy | Scoop.it
The application of matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth expedites the identification of bacteria.
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Bruker Introduces Beta-Lactamase Testing, New Productivity Software Modules ... - SelectScience.net (press release)

Bruker Introduces Beta-Lactamase Testing, New Productivity Software Modules ... - SelectScience.net (press release) | Proteomic Applications in Microbial Taxonomy | Scoop.it
Bruker Introduces Beta-Lactamase Testing, New Productivity Software Modules ...
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Novel Approach for Differentiating Shigella... [J Clin Microbiol. 2013] - PubMed - NCBI

PubMed comprises more than 23 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
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SpringerPlus | Full text | Analysis of functional xylanases in xylan ...

TLC and MALDI-TOF-MS analyses showed that the final hydrolyzed products of the enzyme from beechwood xylan were xylose, xylobiose, and xylotriose substituted with a 4-o-metylglucuronic acid residue. ...
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MALDI-TOF mass spectrometry as a tool for differentiation of Bradyrhizobium species: Application to the identification of Lupinus nodulating strains

MALDI-TOF mass spectrometry as a tool for differentiation of Bradyrhizobium species: Application to the identification of Lupinus nodulating strains | Proteomic Applications in Microbial Taxonomy | Scoop.it

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MALDI Biotyper-Based Rapid Resistance Detection by Stable-Isotope Labeling

Praveen Rahi's insight:

Rapid detection indeed 

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Antibiotic resistance: The last resort

Antibiotic resistance: The last resort | Proteomic Applications in Microbial Taxonomy | Scoop.it
Health officials are watching in horror as bacteria become resistant to powerful carbapenem antibiotics — one of the last drugs on the shelf.
Praveen Rahi's insight:

Hope we can trace these antibiotic resistant bacteria using MALDI-TOF MS

 

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Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections


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MO BIO Laboratories, Inc. launches the first kit for protein extraction from soil - The Sacramento Bee

MO BIO Laboratories, Inc. launches the first kit for protein extraction from soil - The Sacramento Bee | Proteomic Applications in Microbial Taxonomy | Scoop.it
/PRNewswire/ -- MO BIO Laboratories, Inc., the leader in soil, plant, stool and other challenging microbial nucleic acid purification, announces the launch of the NoviPure™ Soil Protein Extraction Kit, for extraction of pure protein from all soil...
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ScienceDirect.com - Trends in Microbiology - On the fitness of microbial taxonomy

Praveen Rahi's insight:

Microbial taxonomy seems to be an expanding discipline especially in Asian countries. By contrast, the number of taxonomists in Western countries are in decline, and the reasons for this could either be economic or due to interest. Losing taxonomy expertise could have consequences for Western countries.

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ScienceDirect.com - Journal of Microbiological Methods - Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification

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