Precise Genome Editing of Livestock
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Precise Genome Editing of Livestock
New advances around genetic manipulation of food animals
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With 'gene-editing' scientists have produced a disease resistant piglet - MercoPress

With 'gene-editing' scientists have produced a disease resistant piglet - MercoPress | Precise Genome Editing of Livestock | Scoop.it
With 'gene-editing' scientists have produced a disease resistant piglet MercoPress 'Gene-editing' involves researchers snipping the animal's DNA and inserting new genetic material, in effect changing a single one of the three billion 'letters' that...

Via Jun Liu
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Proceedings of the Annual Conference of the International Embryo Transfer Society, Hannover, Germany, 19–22 January 2013

Proceedings of the Annual Conference of the International Embryo Transfer Society, Hannover, Germany, 19–22 January 2013 | Precise Genome Editing of Livestock | Scoop.it

VERSATILE TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASE (TALEN)-MEDIATED ENGINEERING OF OSSABAW MINIATURE SWINE 

D. F. Carlson , W. Tan and S. C. Fahrenkrug (pp. 312-312)



NONMEIOTIC INTROGRESSION OF QUANTITATIVE TRAIT NUCLEOTIDES AND CORRECTION OF CONGENITAL MUTATIONS IN LIVESTOCK WITH TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES  
S. C. Fahrenkrug , W. Tan , S. G. Lillico , D. Stverakova , C. Proudfoot , G. Williamson , C. R. Long , C. B. A. Whitelaw and D. F. Carlson (pp. 316-316)



Scott C. Fahrenkrug , Wenfang Tan , Simon G. Lillico , Dana Stverakova , Chris Proudfoot , Gayle Williamson , Charles R. Long , Bruce A. Whitelaw and Daniel F. Carlson (pp. 318-318)
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2012 Biosciences KTN Conference Parallel Sessions: Breeding - Scott Fahrenkrug, Recombinetitics

As an academic, inventor and entrepreneur, Scott Fahrenkrug's focus is on the translation of genome science for the transformation of public health through b...
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Trends in Biotechnology - Precision genetic engineering in large mammals

Trends in Biotechnology - Precision genetic engineering in large mammals | Precise Genome Editing of Livestock | Scoop.it

Precision genetic engineering based on stable chromosomal insertion of exogenous DNA in the genomes of large mammals is immensely important for the development of improved biomedical models, pharmaceutical research and an accelerated breeding progress. Precision genetic engineering requires (i) a known locus of genomic integration, (ii) a defined status of foreign DNA, (iii) that transgene expression is unaffected by neighbouring chromosomal sequences, (iv) endogenous genes are not mutated and (v) no unwanted DNA sequences are present. Recently, advanced molecular techniques exploiting exogenous enzymes have opened the possibilities for more sophisticated genetic engineering. Here, we critically review current developments of enzyme-catalysed approaches for targeted transgenesis in large mammals.

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Precision Editing of Large Animal Genomes

Precision Editing of Large Animal Genomes | Precise Genome Editing of Livestock | Scoop.it

Advances in Genetics,


Transgenic animals are an important source of protein and nutrition for most humans and will play key roles in satisfying the increasing demand for food in an ever-increasing world population. The past decade has experienced a revolution in the development of methods that permit the introduction of specific alterations to complex genomes. This precision will enhance genome-based improvement of farm animals for food production. Precision genetics also will enhance the development of therapeutic biomaterials and models of human disease as resources for the development of advanced patient therapies.

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Efficient TALEN-mediated gene knockout in livestock

Efficient TALEN-mediated gene knockout in livestock | Precise Genome Editing of Livestock | Scoop.it

Transcription activator-like effector nucleases (TALENs) are programmable nucleases that join FokI endonuclease with the modular DNA-binding domain of TALEs. Although zinc-finger nucleases enable a variety of genome modifications, their application to genetic engineering of livestock has been slowed by technical limitations of embryo-injection, culture of primary cells, and difficulty in producing reliable reagents with a limited budget. In contrast, we found that TALENs could easily be manufactured and that over half (23/36, 64%) demonstrate high activity in primary cells. Cytoplasmic injections of TALEN mRNAs into livestock zygotes were capable of inducing gene KO in up to 75% of embryos analyzed, a portion of which harbored biallelic modification. We also developed a simple transposon coselection strategy for TALEN-mediated gene modification in primary fibroblasts that enabled both enrichment for modified cells and efficient isolation of modified colonies. Coselection after treatment with a single TALEN-pair enabled isolation of colonies with mono- and biallelic modification in up to 54% and 17% of colonies, respectively. Coselection after treatment with two TALEN-pairs directed against the same chromosome enabled the isolation of colonies harboring large chromosomal deletions and inversions (10% and 4% of colonies, respectively). TALEN-modified Ossabaw swine fetal fibroblasts were effective nuclear donors for cloning, resulting in the creation of miniature swine containing mono- and biallelic mutations of the LDL receptor gene as models of familial hypercholesterolemia. TALENs thus appear to represent a highly facile platform for the modification of livestock genomes for both biomedical and agricultural applications.

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