Precise Genome Editing in Plants
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Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System - J. Gen. Genomics

Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System - J. Gen. Genomics | Precise Genome Editing in Plants | Scoop.it

(via T. Lahaye, thx)

Liang et al, 2013

Here, we report, for the first time, targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system. We designed five TALENs targeting 4 genes, namely ZmPDS, ZmIPK1A, ZmIPK, ZmMRP4, and obtained targeting efficiencies of up to 23.1% in protoplasts, and about 13.3% to 39.1% of the transgenic plants were somatic mutations. Also, we constructed two gRNAs targeting the ZmIPK gene in maize protoplasts, at frequencies of 16.4% and 19.1%, respectively. In addition the CRISPR/Cas system induced targeted mutations in Zea mays protoplasts with efficiencies (13.1%) similar to those obtained with TALENs (9.1%). Our results show that both TALENs and the CRISPR/Cas system can be used for genome modification in maize.


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Ilyas Muhammad's comment, December 17, 2013 6:19 AM
thnx, Seb
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Adjustment to New Technology Improves Efficiency of Genetic Editing - Harvard Crimson

Adjustment to New Technology Improves Efficiency of Genetic Editing - Harvard Crimson | Precise Genome Editing in Plants | Scoop.it
Adjustment to New Technology Improves Efficiency of Genetic Editing Harvard Crimson Although several older technologies for gene surgery have been developed in the past, the CRISPR/Cas9 system allows scientists to more precisely edit portions of...
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Inheritance and specificity of CRISPR in Arabidopsis over multiple generations - it seems to work efficiently! http://t.co/UfHQcoKuA4
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CRISPR Revolutionizes How Scientists View the Genome | GetSynBio

CRISPR Revolutionizes How Scientists View the Genome | GetSynBio | Precise Genome Editing in Plants | Scoop.it
CRISPR has allowed scientists to execute microsurgeries on not just bacteria genomes but also animal genomes. The CRISPR technology opens the gateway to identifying and curing genome defects and diseases.
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First CRISPR-Tinkered Primates Born - Scientist

Tech Times First CRISPR-Tinkered Primates Born Scientist Researchers achieved precise gene modification in monkeys.CELL, NIU ET AL.Sister macaques Ningning and Mingming are the first born of a cohort of 10 primates whose genomes have been monkeyed...
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Addgene: Calarco Lab CRISPR Plasmids

Addgene: Calarco Lab CRISPR Plasmids | Precise Genome Editing in Plants | Scoop.it

Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco JA. NAT METHODS. 2013 Jun


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Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression -Methods

Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression -Methods | Precise Genome Editing in Plants | Scoop.it

(via T. Schreiber, thx)

Kabadi & Gersbach, 2014

Engineered DNA-binding proteins that can be targeted to specific sites in the genome to manipulate gene expression have enabled many advances in biomedical research. This includes generating tools to study fundamental aspects of gene regulation and the development of a new class of gene therapies that alter the expression of endogenous genes. Designed transcription factors have entered clinical trials for the treatment of human diseases and others are in preclinical development. High-throughput and user-friendly platforms for designing synthetic DNA-binding proteins present innovative methods for deciphering cell biology and designing custom synthetic gene circuits. We review two platforms for designing synthetic transcription factors for manipulating gene expression: Transcription activator-like effectors (TALEs) and the RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system. We present an overview of each technology and a guide for designing and assembling custom TALE- and CRISPR/Cas9-based transcription factors. We also discuss characteristics of each platform that are best suited for different applications.


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Multigeneration analysis reveals the inheritanc...

Multigeneration analysis reveals the inheritanc... | Precise Genome Editing in Plants | Scoop.it
The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has emerged as a powerful tool for targeted gene editing in many organisms, including plants.
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Cas9: The Enzyme, The RNA, & The Virus - YouTube

An educational video short describing research on Cas9.
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Genome-wide prediction of highly specific guide RNA spacers for the CRISPR-Cas9 mediated genome editing in model plants and major crops

Genome-wide prediction of highly specific guide RNA spacers for the CRISPR-Cas9 mediated genome editing in model plants and major crops | Precise Genome Editing in Plants | Scoop.it

Scooped from: Molecular Plant, 2013

Authors: Kabin Xie, Jianwei Zhang and Yinong Yang

 

Genome-wide prediction of highly specific guide RNA (gRNA) spacer sequences  in eight model plants and agricultural crop species, including 

Arabidopsis thaliana, Medicago truncatula, Glycine max (soybean), Solanum lycopersicum (tomato), Brachypodium distachyon, Oryza sativa (rice), Sorghum bicolor and Zea mays (maize).

 

CRISPR-PLANT Database - http://www.genome.arizona.edu/crispr "enables the plant research community to access genome-wide predictions of specific gRNAs, and facilitate the application of CRISPR-Cas9 mediated genome editing in model plants and major agricultural crops".

 


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Improving CRISPR Specificity - Genetic Engineering News

Improving CRISPR Specificity - Genetic Engineering News | Precise Genome Editing in Plants | Scoop.it
Genetic Engineering News
Improving CRISPR Specificity
Genetic Engineering News
Scientists at Massachusetts General Hospital (MGH) say a simple adjustment to a CRISPR may be able to improve its specificity.
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