Plant virology and phytoplasmology
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Association of ‘Candidatus Liberibacter solanacearum’ with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection Teresani et al. 2014

Association of ‘Candidatus Liberibacter solanacearum’ with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection Teresani et al. 2014 | Plant virology and phytoplasmology | Scoop.it

A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with ‘Candidatus Liberibacter solanacearum’ and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect ‘Ca. L. solanacearum’. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of ‘Ca. Liberibacter’ were observed using electron microscopy in celery plant tissues. A fifth haplotype of ‘Ca. L. solanacearum’, named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium.


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Petter Françoise's curator insight, July 12, 2014 3:19 AM

An EPPO Diagnostic protocol is in preparation for ‘Ca. L. solanacearum’. 

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ISFC 2014 - Home

ISFC 2014 - Home | Plant virology and phytoplasmology | Scoop.it
International Stone Fruit Conference International event Stone fruit production ISFC 2014
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A new event for the cultivation of Stone Fruits on Friday 23th of May 2014.

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Methods in virus diagnostics: From ELISA to next generation sequencing Boonham et al 2014

Methods in virus diagnostics: From ELISA to next generation sequencing Boonham et al 2014 | Plant virology and phytoplasmology | Scoop.it

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Petter Françoise's curator insight, January 16, 2014 7:19 AM

A review article involving some  of our regional experts!

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EFSA - Scientific Opinion: Nepoviruses and SLRSV pest risk assessment

EFSA - Scientific Opinion: Nepoviruses and SLRSV pest risk assessment | Plant virology and phytoplasmology | Scoop.it

The EFSA Panel on Plant Health assessed the risk to plant health of Arabis mosaic virus (ArMV), Strawberry latent ringspot virus (SLRSV), Raspberry ringspot virus (RpRSV) and Tomato black ring virus (TBRV) for the EU territory.

These viruses are largely restricted to Europe and their vector nematodes and at-risk hosts occur widely in Europe. Potential impact is rated minimal to minor in all hosts, with the exception of ArMV in grapevine, for which it is minor to moderate.

It is concluded that if the current legislation were removed, no major consequences would be expected unless the industry simultaneously ceased its voluntary certification activity. 


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Muriel Suffert's curator insight, October 11, 2013 4:26 AM

These viruses are currently regulated in the EU for Plants of Fragaria L. and Rubus L., intended for planting.

This risk assessment is part of a programme of reevaluation of pests which have been listed for many years by the EU.

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Bioresources and viruses - Main page

Bioresources and viruses - Main page | Plant virology and phytoplasmology | Scoop.it
Bioresources and viruses - is one of the largest in Eastern Europe forums created specifically for discussion of problems of medicine, veterinary medicine, plant pathology, chemotherapy, environmental and biosafety in the event of new infections -...
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Characterization of Sour Cherry Isolates of Plum pox virus from the Volga Basin in Russia Reveals a New Cherry Strain of the Virus

Characterization of Sour Cherry Isolates of Plum pox virus from the Volga Basin in Russia Reveals a New Cherry Strain of the Virus | Plant virology and phytoplasmology | Scoop.it

Three complete and several partial genomic sequences of PPV isolates from sour cherry trees in the Volga River basin of Russia have been determined. 

 

A specific primer pair has been developed, allowing the specific reverse-transcription polymerase chain reaction detection of PPV-CR isolates.


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Petter Françoise's curator insight, August 9, 2013 6:49 AM

PPV is an EPPO A2 Listed pest and an EPPO protocol is under revision to align it to the IPPC diagnostic protocol adopted in 2012. 

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2nd EPPO Workshop for heads of laboratories

2nd EPPO Workshop for heads of laboratories | Plant virology and phytoplasmology | Scoop.it

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Petter Françoise's curator insight, May 29, 2013 7:24 AM

The main topic was "Plant pest diagnostic laboratories: towards national and regional reference laboratories" 

View the presentations and read the main conclusions and recommendations

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3rd Meeting of the Panel on Diagnostics in Virology and Phytoplasmology

3rd Meeting of the Panel on Diagnostics in Virology and Phytoplasmology | Plant virology and phytoplasmology | Scoop.it

read a short report of the last Panel! 


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Development of a Multiplexed Bead-Based Suspension Array for the Detection and Discrimination of Pospiviroid Plant Pathogens

Development of a Multiplexed Bead-Based Suspension Array for the Detection and Discrimination of Pospiviroid Plant Pathogens | Plant virology and phytoplasmology | Scoop.it

Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagating material are essential for the management of pospiviroid diseases in horticultural crops. This study describes the development of a true multiplexed diagnostic method for the detection and identification of all nine currently recognized pospiviroid species in one assay using Luminex bead-based suspension array technology.


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Petter Françoise's curator insight, January 15, 2014 3:10 AM

The EPPO Panel on Diagnostics in Virology and Phytoplasmology agreed at its last meeting that a  generic Standard on the detection of pospiviroids should be prepared for the next meeting of the Panel

http://www.eppo.int/MEETINGS/2013_meetings/virology.htm

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EPPO Workshop on setting Ct cut-off values for real-time PCR Paris, 2013-11-11/12

EPPO Workshop on setting Ct cut-off values for real-time PCR Paris, 2013-11-11/12 | Plant virology and phytoplasmology | Scoop.it

Read the conclusions of the Workshop


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Petter Françoise's curator insight, November 26, 2013 11:29 AM

The Workshop allowed the improvement of the intructions to authors of EPPO Diagnostic Protocols. 

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Joint EFSA-EPPO Workshop on ‘Data collection and information sharing in plant health’, Parma, Italy, 1-3 April 2014

Joint EFSA-EPPO Workshop on ‘Data collection and information sharing in plant health’, Parma, Italy, 1-3 April 2014 | Plant virology and phytoplasmology | Scoop.it

A joint EFSA-EPPO Workshop will be organized on 2014-04-01/03 in Parma on ‘Data collection and information sharing in plant health’.

Data collection is a fundamental element for an evidence based risk assessment and for decision making in plant health. Much risk assessment uncertainty is due to lack of data or to unavailable data.
The main objective of this Workshop is to exchange experience and reflect together on how to collect, store and disseminate plant health information at national, regional and global level.

The Workshop will include presentations from key note speakers and participants, as well as posters session. It is also planned to allow sufficient time for discussion.


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Muriel Suffert's curator insight, October 22, 2013 4:34 AM

Interested participants are kindly requested to express their interest emailing their pre-registration and abstracts submission form by 31 October 2013.

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2nd International Symposium on Plum Pox Virus [Sharka Virus], September 3-6, 2013 Olomouc- Czech Repulic 2013


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Ciheam-Bari FTN Press-Review's curator insight, August 27, 2013 10:05 AM

2nd International Symposium on Plum Pox Virus- September 3-6, 2013 Olomouc- Czech Repulic


The 2nd International Symposium on PlumPox Virus (2ndISPPV)  will be organized in Olomouc, in the Czech Republic. The sym posium is organized under auspices of International Society for Horticultural Science (ISHS), Czech Society for Plant Pathology and representatives of the Palacký University and Olomouc city.

The main aim of the Symposium is to bring together scientists, practical breeders and students interested in Plum pox virus, important quarantine pathogen of stone fruittrees and give them the possibility to change the knowledge and ideas about the topic.The main aspects the Symposium would like to cover are:1. PPV distribution, diversity and epidemiology ( worldwide occurrence, biological and molecular variability, phylogeny, temporal and spatial spreading) Plenary lecture: Thiery Candresse (INRA, Université de Bordeaux, France) – A global vision of PPV diversity: things we know and things we don‘t (yet) know
2. PPV resistance ( PPV-host interactions, molecular markers for assisted selection, conventional breeding, biotechnological approach for PPV resistance ) Plenary lecture: Veronique Decroocq (INRA, Université de Bordeaux, France) –Translational research using OLD and NEW generation technologies to develop Plum pox virus-resistant Prunus crops3. PPV control and management (advances in detections, diagnosis and identification, cultivation of tolerant and resistant cultivars, agro-technical measures and cultivation guidelines) Plenary lecture: Mariano Cambra (IVIA, Spain) – Diagnosis and detection of Plum poxvirus. State of the art and future options.The 2nd ISPPV would like to join the history of the Sharka research represented by the Middle European Meeting on Plum Pox Virus and its future that was opened in the 1st International Symposium on Plum Pox Virus established in Sofia, Bulgary in 2010.
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IPPC Technical Panel on Diagnostic Protocols at EPPO headquarters

IPPC Technical Panel on Diagnostic Protocols at EPPO headquarters | Plant virology and phytoplasmology | Scoop.it

The Technical Panel on Diagnostic Protocol of the International Plant Protection Convention met at the EPPO headquarters this week. It reviewed the following drafts international diagnostic protocols:

Anastrepha spp. 

Tospoviruses (TSWV, INSV, WSMV)

Phytoplasmas (general) 

Ditylenchus destructor / D. dipsaci

Sorghum halepense

 

It also discussed the drafts on Erwina amylovora and Tilletia indica. 

It also reviewed its work plan. It is expected that approximately 15 protocols will be presented for member consultation by 2016.

 

Two IPPC diagnostic protocols will be sent for member consultation on the 1st of July (PSTVd and Xanthomonas citri)

 

Members of the TPDP present at the meeting:

Ms Jane CHARD (GBR) Steward

Mr Robert TAYLOR (NZL) Bacteriology
Ms Géraldine ANTHOINE (FRA)  Nematology
Mr Delano JAMES (CAN) Virology
Mr Brendan RODONI (AUS) Virology and backup bacteriology
Ms Liping YIN (CHN) Botany

 

Other members (not present)

Ms Ana Lía TERRA (URY) Entomology
Mr Norman B. BARR (USA) Entomology
Mr Johannes DE GRUYTER (NLD) Mycology

 

Ms Mehle (NIB, SI) (Phytoplasmology and Virology) was invited as host representative. 


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Petter Françoise's curator insight, June 28, 2013 2:17 AM

EPPO is following closely the development of IPPC protocols in order to align its protocols with these international standards and avoid duplication of work.

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2nd meeting of the EPPO Panel on Diagnostics in Virology and Phytoplasmology

2nd meeting of the EPPO Panel on Diagnostics in Virology and Phytoplasmology | Plant virology and phytoplasmology | Scoop.it

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Petter Françoise's curator insight, May 29, 2013 7:22 AM

Read a short report about the achievements and furture programme of the Panel