WA wheatgrowers have been advised to monitor crops throughout the season for signs of leaf rust following testing which confirmed an increased risk of rust in popular wheat varieties. Department of Agriculture and Food plant pathologist Geoff Thomas said that in 2013, wheat leaf rust with increased virulence for several popular varieties was discovered in WA. Testing through the Australian Cereal Rust Control Program at Sydney University confirmed the presence of the leaf rust pathotype named 76-1, 3, 5, 7, 9, 10, 12 +Lr 37. "This represents the first occurrence in WA of virulence for varieties which contain Lr13 resistance," Mr Thomas said. "As a result, several varieties, including Mace, are expected to have an altered response in the presence of this pathotype. "In order to gauge the rust response of wheat varieties popular in WA, the Department recently completed glasshouse trials to test the seedling and adult reactions of varieties to this new pathotype. "Our pathology tests confirmed that varieties Mace, Wyalkatchem and Corack, which all carry Lr13, are susceptible (S) as seedlings and moderately susceptible to susceptible (MS-S) as adult plants. "This result signifies a significant reduction in resistance from the moderately-resistant (MR) response of these varieties to the former dominant rust pathotype in WA.
Plant-Pathogen Interactions: Methods and Protocols, Second Edition by Paul Birch, John Jones, Jorunn Bos (Editors) expands upon the first edition with current, detailed protocols for the study of plant pathogen genome sequences. It contains new chapters on techniques to help identify and characterize effectors and to study their impacts on host immunity and their roles in pathogen biology. Additional chapters focus on protocols to identify avirulence and resistance genes, investigate the roles of effector targets and other defence-associated proteins in plant immunity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
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