Zhongwei Lin,Xianran Li,Laura M Shannon,Cheng-Ting Yeh,Ming L Wang,Guihua Bai,Zhao Peng,Jiarui Li,Harold N Trick,Thomas E Clemente,John Doebley,Patrick S Schnable,Mitchell R Tuinstra,Tesfaye T Tesso,Frank White& Jianming Yu
Received18 January 2012Accepted19 April 2012Published online13 May 2012
A key step during crop domestication is the loss of seed shattering. Here, we show that seed shattering in sorghum is controlled by a single gene, Shattering1 (Sh1), which encodes a YABBY transcription factor. Domesticated sorghums harbor three different mutations at the Sh1 locus. Variants at regulatory sites in the promoter and intronic regions lead to a low level of expression, a 2.2-kb deletion causes a truncated transcript that lacks exons 2 and 3, and a GT-to-GG splice-site variant in the intron 4 results in removal of the exon 4. The distributions of these non-shattering haplotypes among sorghum landraces suggest three independent origins. The function of the rice ortholog (OsSh1) was subsequently validated with a shattering-resistant mutant, and two maize orthologs (ZmSh1-1 and ZmSh1-5.1+ZmSh1-5.2) were verified with a large mapping population. Our results indicate that Sh1 genes for seed shattering were under parallel selection during sorghum, rice and maize domestication.