Plant cell culture
Follow
Find
16 views | +0 today
Your new post is loading...
Your new post is loading...
Rescooped by Fernando Cardoso from Plants&Bacteria
Scoop.it!

Identification and Characterization of In planta–Expressed Secreted Effector Proteins from Magnaporthe oryzae That Induce Cell Death in Rice

Identification and Characterization of In planta–Expressed Secreted Effector Proteins from Magnaporthe oryzae That Induce Cell Death in Rice | Plant cell culture | Scoop.it

Summary:

Interactions between rice and Magnaporthe oryzae involve the recognition of cellular components and the exchange of complex molecular signals from both partners. How these interactions occur in rice cells is still elusive. We employed robust-long serial analysis of gene expression, massively parallel signature sequencing, and sequencing by synthesis to examine transcriptome profiles of infected rice leaves. A total of 6,413 in planta–expressed fungal genes, including 851 genes encoding predicted effector proteins, were identified. We used a protoplast transient expression system to assess 42 of the predicted effector proteins for the ability to induce plant cell death. Ectopic expression assays identified five novel effectors that induced host cell death only when they contained the signal peptide for secretion to the extracellular space. Four of them induced cell death in Nicotiana benthamiana. Although the five effectors are highly diverse in their sequences, the physiological basis of cell death induced by each was similar. This study demonstrates that our integrative genomic approach is effective for the identification of in planta–expressed cell death–inducing effectors from M. oryzae that may play an important role facilitating colonization and fungal growth during infection.


Via Freddy Monteiro
more...
No comment yet.
Rescooped by Fernando Cardoso from The Asian Food Gazette.
Scoop.it!

WILD PLANTS USED AS MEDICINAL PURPOSE IN THE SOUTH PART OF İZMİT (NORTHWEST TURKEY)

WILD PLANTS USED AS MEDICINAL PURPOSE IN THE SOUTH PART OF İZMİT (NORTHWEST TURKEY) | Plant cell culture | Scoop.it
In this study, local names, medicinal usage and methods of administration of 64 wild plant taxa belonging to 32 families in the south part of Izmit, which is the centre county of Kocaeli, are documented.

Via Frank Kusters
more...
No comment yet.
Rescooped by Fernando Cardoso from Rhizobium Research
Scoop.it!

Partial complementation of Sinorhizobium meliloti bacA mutant phenotypes by the Mycobacterium tuberculosis BacA protein

The Sinorhizobium meliloti BacA ABC transporter protein plays an important role in its nodulating symbiosis with the legume alfalfa (Medicago sativa). The Mycobacterium tuberculosis BacA homolog was found to be important for the maintenance of chronic murine infections, yet its in vivo function is unknown. In the legume plant as well as in the mammalian host, bacteria encounter host antimicrobial peptides (AMPs). We found that the M. tuberculosis BacA protein was able to partially complement the symbiotic defect of an S. meliloti BacA-deficient mutant on alfalfa plants and to protect this mutant in vitro from the antimicrobial activity of a synthetic legume peptide, NCR247 and a recombinant human β-defensin 2 (HBD2). This finding was also confirmed using an M. tuberculosis insertion mutant. Furthermore, M. tuberculosis BacA-mediated protection of the legume symbiont S. meliloti against legume defensins as well as HBD2 is dependent on its attached ATPase domain. In addition, we show that M. tuberculosis BacA mediates peptide uptake of the truncated bovine AMP, Bac71-16. This process required a functional ATPase domain. We therefore suggest that M. tuberculosis BacA is important for the transport of peptides across the cytoplasmic membrane and is part of a complete ABC transporter. Hence, BacA-mediated protection against host AMPs might be important for the maintenance of latent infections.

 

M.F.F Arnold, A.F. Haag, S. Capewell, H.I. Boshoff, E.K. James, R. McDonald, I. Mair, A.M. Mitchell, B. Kerscher, T.J. Mitchell, P. Mergaert, C.E. Barry 3rd, M. Scocchi, M. Zanda, D.J. Campopiano and G.P. Ferguson (2012).  JBact accepts  Nov. 16

 

 


Via IvanOresnik
more...
No comment yet.