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Quantitative analysis of “flavescence doreé” phytoplasma with droplet digital PCR Mehle et al 2014

Quantitative analysis of “flavescence doreé” phytoplasma with droplet digital PCR Mehle et al 2014 | Phytoplasma | Scoop.it

The first droplet-digital-PCR-based absolute quantification of “flavescence dorée” phytoplasma, agent of a quarantine phytoplasma yellows in grapevine was performed. Quantitative PCR that targets the secY gene was transferred to droplet digital PCR and used for absolute quantification of “flavescence dorée” phytoplasma, without the need for calibration curves. The sensitivity of the two assays compares well and was shown that it could be used for quantification and quality control of DNA based on in-house reference materials typically used in diagnostics and metrological laboratories. This new tool has great potential for monitoring phytoplasma kinetics, such as there relationship with the progress of an infection, and variations of the phytoplasma titer through the season and screening plants for resistance.


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Petter Françoise's curator insight, August 7, 2014 10:07 AM

The EPPO standards PM 7/79(1) Grapevine flavescence dorée phytoplasma is under revision. 

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The potential ecological impact of ash dieback in the UK

The potential ecological impact of ash dieback in the UK | Phytoplasma | Scoop.it

The Joint Nature Conservation Committee formed a consortium to to improve understanding of the impacts of ash dieback on biodiversity in the UK.

The purpose of this report is to provide supporting scientific information to help inform the policy makers, land managers and advisers involved in management of woodlands and trees so that potential impacts of ash dieback on biodiversity are appropriately understood and considered.  This will help to promote management practices that will conserve and enhance biodiversity and the delivery of ecosystem services. 

The report gives an analysis of information about the ecology and biodiversity of ash (Fraxinus excelsior) considered in the context of ash dieback, caused by a fungus, ‘Chalara’ (Chalara fraxinea or Hymenoscyphus pseudoalbidus).

 

Mitchell, R.J. et al. 2014 JNCC Report 483  The potential ecological impact of ash dieback in the UK http://jncc.defra.gov.uk/pdf/JNCC483_web.pdf


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First report of a 'Candidatus Phytoplasma ulmi' isolate affecting sapodilla trees in western Cuba

The on-line peer-reviewed journal for new and signficant plant disease outbreaks
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First report on the molecular identification of the phytoplasma associated with a lethal yellowing-type disease of coconut palms in Cote d'Ivoire

The on-line peer-reviewed journal for new and signficant plant disease outbreaks (1st molec. identification of phytoplasma ass.
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Genetic diversity of European phytoplasmas of the 16SrV taxonomic group and proposal of 'Candidatus Phytoplasma rubi'

Genetic diversity of European phytoplasmas of the 16SrV taxonomic group and proposal of 'Candidatus Phytoplasma rubi' | Phytoplasma | Scoop.it
Genetic diversity of European phytoplasmas of the 16SrV taxonomic group and proposal of 'Candidatus Phytoplasma rubi'
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A new disease of banana? : News and analysis | Promusa - Mobilizing banana science for sustainable livelihoods

A new disease of banana? : News and analysis | Promusa - Mobilizing banana science for sustainable livelihoods | Phytoplasma | Scoop.it
A new disease of banana? - News and analysis
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Very Interesting!!!!!!

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BMC Genomics | Full text | Novel aspects of grapevine response to phytoplasma infection investigated by a proteomic and phospho-proteomic approach with data integration into functional networks

Translational and post-translational protein modifications play a key role in the response of plants to pathogen infection.
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Diagnostic techniques for detection of phytoplasma diseases: past and present | Nejat | Journal of Plant Diseases and Protection (JPDP)

Diagnostic techniques for detection of phytoplasma diseases: past and present
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fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0052092&representation=PDF

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UK rapid PRA on ‘Candidatus Phytoplasma ulmi’

The pathogen is listed in the EU Plant Health Directive, (as Elm phloem necrosis mycoplasma), because of its lethal effects on native elm species in North America, although findings on European elm species have tended to be less severe to date (general decline, stunting, leaf yellowing).

Despite the EU listing there have been a number of outbreaks in the EU (Italy, France and Germany). While there is a general requirement to prohibit the movement of the pathogen, the movement of elm planting material in the EU is not regulated and movements do occur between Member States, not least in material found by breeders to be resistant to Dutch Elm Disease. Some of these breeding programmes are located in areas known to be infected with the pathogen.

 

The aim of this consultation is to determine which of the two policy options presented would be the most appropriate response to the outbreak.

Forest Research 2014. Rapid Pest Risk Analysis for Elm Yellows http://www.fera.defra.gov.uk/plants/plantHealth/pestsDiseases/documents/elmYellowsPRA.pdf


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Muriel Suffert's curator insight, February 27, 2014 3:55 AM

In December 2013 the first symptoms in the UK of an elm yellows outbreak were notified in Dutch elm disease resistant plants propagated from Italian parent plants imported in 2012. The outbreak was confirmed by laboratory analysis in late January 2014 and eradication work is on-going.

Comments are sought by 9 April 2014.

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A Multiplex-PCR Method for Strain Identification and Detailed Phylogenetic Analysis of AY-Group Phytoplasmas

A Multiplex-PCR Method for Strain Identification and Detailed Phylogenetic Analysis of AY-Group Phytoplasmas | Phytoplasma | Scoop.it

this multiplex-PCR system is useful for rapid strain identification and detailed phylogenetic analysis of phytoplasmas.


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Petter Françoise's curator insight, February 9, 2014 4:19 AM

Aster yellows (AY) group (16SrI) phytoplasmas are associated with over 100 economically important diseases worldwide and represent the most diverse and widespread phytoplasma group.

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First report of a 'Candidatus Phytoplasma asteris' isolate affecting a strawberry nursery in Cuba

The on-line peer-reviewed journal for new and signficant plant disease outbreaks
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qPCR Experience® workshop: Real-time PCR in Plant pathology: Diagnostics and Research, Ljubljana, Slovenia, 3rd - 6th December 2013 | BioSistemika

qPCR Experience® workshop:

Real-time PCR in Plant pathology - Diagnostics and Research

 

Ljubljana, Slovenia, 3rd - 6th December 2013

 

 

Real-time PCR in Plant pathology will focus on diagnostics of plant pathogenic microorganisms and basics of relative quantification using real-time PCR integrating EPPO Guidelines.

 

Workshop is organised by National Institute of Biology (NIB) and BioSistemika Ltd.

 

Guest lecturers: Françoise Petter, EPPO and Sebastian Kiewnick, Agroscope.

Instructors: Dr. Tanja Dreo, Uni. Prof. Dr. Maja Ravnikar, Mag. Nataša Mehle,

Dr. Manca Pirc, Dr. Urška Čepin

 

The hands-on 4-day workshop will cover review of qPCR theory, LAMP technology, practical guidance experiment set-up,  plant sample preparation and quality control, assay design, automatisation, validation of diagnostic qPCR methods according to EPPO guidelines, as well as hands-on lab and data analysis sessions.

 

Extensive practical sessions will be held in ISO 9001 and ISO 17025 accredited laboratories of National Institute of Biology in Ljubljana which includestate-of-the-art qPCR centre. 

 

Join the international team of scientisis, experienced instructors and plant pathology specialists from the Department of Biotechnology and Systems Biology at National Institute of Biology.

 

Registrations and more information available here:  http://www.biosistemika.com/PP12_2013

 

Contact: info@biosistemika.com

 


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Petter Françoise's curator insight, October 3, 2013 4:03 AM

New training session on real-time PCR. Previous sessions have attracted many participants. 

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Root wilt disease proves a tough nut to crack - The Hindu

Root wilt disease proves a tough nut to crack - The Hindu | Phytoplasma | Scoop.it
Root wilt disease proves a tough nut to crack
The Hindu
The etiology of root wilt was established as 'phytoplasma' and its molecular detection was achieved by scientists of Central Plantation Crops Research Institute, said V.
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Phytoplasma (aster yellows) identified on Michigan soybeans | MSU Extension

Phytoplasma (aster yellows) identified on Michigan soybeans | MSU Extension | Phytoplasma | Scoop.it
Phytoplasma, a specialized bacteria capable of causing yield loss, has been observed in a Michigan soybean field during the 2012 season.
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Development of a diagnostic protocol for Flavescence dorée phytoplasma (FDp)

Development of a diagnostic protocol for Flavescence dorée phytoplasma (FDp) | Phytoplasma | Scoop.it
GWRDC invests in and directs research, development and extension that supports a competitive Australian wine sector.
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I have a dream, walk through a vineyard...

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JIC training to help scientists fight disease-causing bacteria | News from the John Innes Centre

JIC training to help scientists fight disease-causing bacteria | News from the John Innes Centre | Phytoplasma | Scoop.it
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We need prepare one !!!!

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‘Candidatus Phytoplasma pruni’, a novel taxon associated with X-disease of stone fruits, Prunus spp.: multilocus characterization based on 16S rRNA, secY, and ribosomal protein genes

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Phytoplasma - Methods and Protocols

Phytoplasma - Methods and Protocols | Phytoplasma | Scoop.it
Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply.
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Molecular identification of phytoplasma vec... [Methods Mol Biol. 2013] - PubMed - NCBI

PubMed comprises more than 23 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
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IPWG - International Phytoplasmologist Working Group

IPWG - International Phytoplasmologist Working Group | Phytoplasma | Scoop.it
International Phytoplasmologist Working Group Web site
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How can I be part of this Group?

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