physiological probes and assays
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Cal-520™ Calcium Indicators, the Best 488 nm-Excitable Green Fluorescent Calcium Indicators with The Highest Signal to Background Ratio

Cal-520™ Calcium Indicators, the Best 488 nm-Excitable Green Fluorescent Calcium Indicators with The Highest Signal to Background Ratio | physiological probes and assays | Scoop.it
AAT Bioquest's insight:

Cal-520™ provides the most robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520™ AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to background ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). The higher signal/background ratio and better intracellular retention make the Cal-520™ calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.

 

Our preliminary in-house research indicated that Cal-520™ AM can be readily loaded to a guinea pig heart and stays there for a few hours in the absence of probenecid. The calcium signal can be readily monitored with Cal-520™ AM while it is difficult to observe the calcium signal under the same conditions with other green calcium dyes such as Fluo-3 AM and Fluo-4 AM.

 

Key Features of Cal-520™ AM:

 

 •    Higher Sensitivity, Cal-520 AM has much higher signal/background ratio than Fluo-3 AM and Fluo-4 AM in cells.

  •    Better  Intracellular Retention, Cal-520 AM is better retained in llive cells than Fluo-3 AM and Fluo-4 AM in cells.

  •    Convenient, Cal-520 AM has almost identical spectra to those of Fluo-4 AM.

 

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Fluo-8® Calcium Indicators, the Brightest 488 nm-Excitable Green Fluorescent Calcium Indicators

Fluo-8® Calcium Indicators, the Brightest 488 nm-Excitable Green Fluorescent Calcium Indicators | physiological probes and assays | Scoop.it
AAT Bioquest's insight:

Fluo-3 and Fluo-4 were the most commonly used visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis, and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes have been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of maximum excitation @ ~490 nm and maximum emission @ ~520 nm. For cell loading, Fluo-8® AM only requires incubation at room temperature while Fluo-3 AM and Fluo-4 AM require incubation at 37 oC. In addition, Fluo-8® AM is 2 times brighter than Fluo-4 AM, and 4 times brighter than Fluo-3 AM in cells. AAT Bioquest offers a set of outstanding Fluo-8® reagents with different calcium binding affinities.

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