|Scooped by AAT Bioquest|
Cal-520™ Calcium Indicators, the Best 488 nm-Excitable Green Fluorescent Calcium Indicators with The Highest Signal to Background Ratio
Cal-520™ provides the most robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520™ AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to background ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). The higher signal/background ratio and better intracellular retention make the Cal-520™ calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.
Our preliminary in-house research indicated that Cal-520™ AM can be readily loaded to a guinea pig heart and stays there for a few hours in the absence of probenecid. The calcium signal can be readily monitored with Cal-520™ AM while it is difficult to observe the calcium signal under the same conditions with other green calcium dyes such as Fluo-3 AM and Fluo-4 AM.
Key Features of Cal-520™ AM:
• Higher Sensitivity, Cal-520 AM has much higher signal/background ratio than Fluo-3 AM and Fluo-4 AM in cells.
• Better Intracellular Retention, Cal-520 AM is better retained in llive cells than Fluo-3 AM and Fluo-4 AM in cells.
• Convenient, Cal-520 AM has almost identical spectra to those of Fluo-4 AM.