Noncoding RNA
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Rescooped by Hong Jiang from DNA and RNA Research
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‘Gene Surgery’ cures disease in mammals

‘Gene Surgery’ cures disease in mammals | Noncoding RNA | Scoop.it

Biologists have now used CRISPR/Cas9 to edit the genome of mice with muscular dystrophy. 


Via Integrated DNA Technologies
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Rescooped by Hong Jiang from TAL effector science
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TALEN-based knockout library for human microRNAs - Nature Struc. & Mol. Biol.

TALEN-based knockout library for human microRNAs - Nature Struc. & Mol. Biol. | Noncoding RNA | Scoop.it

(via T. Schreiber & T. Lahaye, thx)

Kim et al, 2013

Various technical tools have been developed to probe the functions of microRNAs (miRNAs), yet their application has been limited by low efficacy and specificity. To overcome the limitations, we used transcription activator–like effector nucleases (TALENs) to knock out human miRNA genes. We designed and produced a library of 540 pairs of TALENs for 274 miRNA loci, focusing on potentially important miRNAs. The knockout procedure takes only 2–4 weeks and can be applied to any cell type. As a case study, we generated knockout cells for two related miRNAs, miR-141 and miR-200c, which belong to the highly conserved miR-200 family. Interestingly, miR-141 and miR-200c, despite their overall similarity, suppress largely nonoverlapping groups of targets, thus suggesting that functional miRNA-target interaction requires strict seed-pairing. Our study illustrates the potency of TALEN technology and provides useful resources for miRNA research.


Via dromius
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Scooped by Hong Jiang
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Human 4E-T represses translation of bound mRNAs and enhances microRNA-mediated silencing

Human 4E-T represses translation of bound mRNAs and enhances microRNA-mediated silencing | Noncoding RNA | Scoop.it
Human 4E-T represses translation of bound mRNAs and enhances microRNA-mediated silencing: A key player in... http://t.co/46gviQxS0J #NAR
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Rescooped by Hong Jiang from DNA and RNA Research
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CRISPR and Cas9 for Flexible Genome Editing

CRISPR and Cas9 for Flexible Genome Editing | Noncoding RNA | Scoop.it

CRISPR RNAs Make Targeted Gene Editing Easy


Via Integrated DNA Technologies
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Rescooped by Hong Jiang from Stem Cells & Tissue Engineering
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Inhibition of let-7 microRNA promotes reprogramming to human induced pluripotent stem cells (iPSC) - Cell Stem Cell


Via Jacob Blumenthal
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Jacob Blumenthal's curator insight, November 16, 2013 7:16 AM

A new paper from the lab of Shinya Yamanaka suggest that the let-7  family of microRNAs induce an inhibitory effect on the reprogramming process by regulation of pro-differentiation factors, such as EGR1. Inhibition of let-7  in human cells results in an increase in the level of the let-7 target LIN-41/TRIM71, which in turn promotes reprogramming.

http://www.cell.com/cell-stem-cell/abstract/S1934-5909(13)00492-X


To learn about stem cells and related differentiation protocols, follow the links:

http://discovery.lifemapsc.com/stem-cell-differentiation/in-vitro-cells

http://discovery.lifemapsc.com/stem-cell-differentiation/protocols


Scooped by Hong Jiang
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Cell - Direct Conversion of Fibroblasts to Neurons by Reprogramming PTB-Regulated MicroRNA Circuits

Cell - Direct Conversion of Fibroblasts to Neurons by Reprogramming PTB-Regulated MicroRNA Circuits | Noncoding RNA | Scoop.it
From Cell: Direct Conversion of Fibroblasts to Neurons by Reprogramming PTB-Regulated MicroRNA Circuits http://t.co/Rraf7rQH -NK
Hong Jiang's insight:

might be a new direction.

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