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Rescooped by Mark Bayliss from Monsanto Sucks
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Genetically-modified purple tomatoes heading for shops - BBC News

Genetically-modified purple tomatoes heading for shops - BBC News | NMR | Scoop.it
“ BBC News Genetically-modified purple tomatoes heading for shops BBC News The prospect of genetically modified purple tomatoes reaching the shelves has come a step closer.”
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Rescooped by Mark Bayliss from Mass spectrometry
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Metabolites | Free Full-Text | A Comprehensive Workflow of Mass Spectrometry-Based Untargeted Metabolomics in Cancer Metabolic Biomarker Discovery Using Human Plasma and Urine

Metabolites | Free Full-Text | A Comprehensive Workflow of Mass Spectrometry-Based Untargeted Metabolomics in Cancer Metabolic Biomarker Discovery Using Human Plasma and Urine | NMR | Scoop.it
“ Current available biomarkers lack sensitivity and/or specificity for early detection of cancer. To address this challenge, a robust and complete workflow for metabolic profiling and data mining is described in details.”
Via Edwin De Pauw
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This is an excellent article for anyone interested in understanding the mechanics of doing metabolomics data reduction and results interpretation. The article clearly highlights the challenges that still exist in this space and the clear need to use multiple strategies and analytical tools to solve the global problem.
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Rescooped by Mark Bayliss from Viruses and Bioinformatics from Virology.uvic.ca
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BMC Bioinformatics | Abstract | MultiAlign: a multiple LC-MS analysis tool for targeted omics analysis

“ MultiAlign is a free software tool that aligns multiple liquid chromatography-mass spectrometry datasets to one another by clustering mass and chromatographic elution features across datasets.”
Via Chris Upton + helpers
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It is great to see additional efforts within the community to find practical solutions to aligning complex data. This is not an easy thing to get right. Especially in non-linear time alignment space.
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Rescooped by Mark Bayliss from COOL 3DPRINTING
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'Body on a chip' used for drug tests

'Body on a chip' used for drug tests | NMR | Scoop.it
A technology which puts miniature human organs made with a 3D printer on a microchip is being used to test new drugs and vaccines.Miniature human organs developed with a modified 3D printer are being used to test new vaccines in a lab in the US.The "body on a chip" project replicates human cells to print structures which mimic the functions of the heart, liver, lung and blood vessels.The organs are then placed on a microchip and connected with a blood substitute, allowing scientists to closely monitor specific treatments.The US Department of Defense has backed the new technology with $24m (£15m).Bioprinting, a form of 3D printing which, in effect, creates human tissue, is not new. Nor is the idea of culturing 3D human tissue on a microchip.
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Via Annie Theunissen
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Rescooped by Mark Bayliss from Viruses and Bioinformatics from Virology.uvic.ca
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Wow of the week: With a little bioengineering, E. coli becomes a pathogen-fighting superhero

Wow of the week: With a little bioengineering, E. coli becomes a pathogen-fighting superhero | NMR | Scoop.it
“ Researchers may have found a way to turn E. coli bacteria into a way to fight hospital-acquired infections like pneumonia and bloodstream infections.”
Via Gerd Moe-Behrens, Pedro Fernandes, Chris Upton + helpers
Mark Bayliss's insight:
This would have huge potentials for fighting infections that are getting harder to treat with antibiotics.
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Rescooped by Mark Bayliss from Natural Products Chemistry Breaking News
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Accurate mass–time tag library for LC/MS-based metabolite profiling of medicinal plants

Accurate mass–time tag library for LC/MS-based metabolite profiling of medicinal plants | NMR | Scoop.it
We report the development and testing of an accurate mass–time (AMT) tag approach for the LC/MS-based identification of plant natural products (PNPs) in complex extracts. An AMT tag library was developed for approximately 500 PNPs with diverse chemical structures, detected in electrospray and atmospheric pressure chemical ionization modes (both positive and negative polarities). In addition, to enable peak annotations with high confidence, MS/MS spectra were acquired with three different fragmentation energies. The LC/MS and MS/MS data sets were integrated into online spectral search tools and repositories (Spektraris and MassBank), thus allowing users to interrogate their own data sets for the potential presence of PNPs. The utility of the AMT tag library approach is demonstrated by the detection and annotation of active principles in 27 different medicinal plant species with diverse chemical constituents. Daniel J. Cuthbertsona, 1,Sean R. Johnsona,Jasenka Piljac-Žegaraca, b,Julia Kappela, c,Sarah Schäfera, c,Matthias Wüstc,Raymond E.B. Ketchuma, 2,Rodney B. Croteaua,Joaquim V. Marquesa,Laurence B. Davina,Norman G. Lewisa,Megan Rolfd,Toni M. Kutchand,D. Doel Soejartoe, f,B. Markus Langea, , Phytochemistry, in Press
Via NatProdChem
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Rescooped by Mark Bayliss from Science News
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Mosquitoes That Are Genetically-Engineered to Self Destruct (To Prevent Disease) Are in the Air

Mosquitoes That Are Genetically-Engineered to Self Destruct (To Prevent Disease) Are in the Air | NMR | Scoop.it
“ A new breed of genetically modified mosquitoes carries a gene that cripples its own offspring. They could crush native mosquito populations and block the spread of disease.”
Via Sakis Koukouvis
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Rescooped by Mark Bayliss from News You Can Use - NO PINKSLIME
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Mosquito 'invisibility cloak' found

Mosquito 'invisibility cloak' found | NMR | Scoop.it
“ A naturally occurring substance found in human skin could yield a viable alternative to existing mosquito repellent, scientists say.”
Via #BBBundyBlog #NOMORELIES Tom Woods #Activist Award #Scoopiteer >20,000 Sources >250K Connections http://goo.gl/ruHO3Q
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Rescooped by Mark Bayliss from Mass spectrometry
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Cleaning up the masses: Exclusion lists to reduce contamination with HPLC-MS/MS

Cleaning up the masses: Exclusion lists to reduce contamination with HPLC-MS/MS | NMR | Scoop.it

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Inhibition of neuroinflammation by cinnamon and its main components. [Food Chem. 2013] - PubMed - NCBI

Inhibition of neuroinflammation by cinnamon and its main components. [Food Chem. 2013] - PubMed - NCBI | NMR | Scoop.it
Abstract
Uncontrolled activation of microglia contributes to neuroinflammation, which is highly involved in the development of neurodegenerative diseases. Although cinnamon has neuro-protective properties, its capacity to inhibit neuroinflammation has not been investigated and its active compounds remain unclear. Therefore, the composition of cinnamon extract was analysed by LC-MS and the ability of cinnamon and its main constituents to inhibit neuroinflammation was evaluated using a lipopolysaccharide (LPS)-activated BV2 microglia culture system. In total, 50 μg/mL cinnamon extract decreased significantly the production and expression of nitric oxide (NO), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in LPS-activated BV2 microglia. Blocking of nuclear factor-κB (NF-κB) activation was the most likely mechanism responsible for inhibition by cinnamon of neuroinflammation. Among the eight tested compounds, cinnamaldehyde had the greatest anti-neuroinflammatory capacity. Experimental results suggest that cinnamon may have a potential therapeutic effect against neurodegenerative diseases and its potent anti-neuroinflammatory capacity was primarily attributed to cinnamaldehyde.
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A spicy diet is really a good thing.
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Rescooped by Mark Bayliss from Mass Spectrometry Geekery
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Designing transitions: Skyline

Designing transitions: Skyline | NMR | Scoop.it
The strength of the triple quad is to use prior knowledge of how peptides (in my case) fragment to detect them reproducibly in complex mixtures. This is a critical and non-trivial step. I am using Skyline - a wonderful open source program developed by the MacCoss Lab - to mine our extensive collection of peptide spectra collected on our Orbitrap and develop methods for the TQS. So far this is working pretty well; many transitions from the standard UPS2 mixture have behaved. Stright off the bat I am down to 500 attomoles on column. When I optimise the spray position more, change to a better column, define retention tiems and play with collision energies I think 5 attomoles is within our reach. Things have gone so well that I am now looking at a 'real' protein and trying to have a quick look at some phosphopeptides before I go on holiday. [edit Apil 2012 - actually 5 attomoles on column is still below my reach. 100 attomoles, yes if everything is in tip-top condition (clean but not absorbant column, clean source, stable spray, no stray polymers...oh yes and nice tight peaks - no post-column dead volume!)]
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Rescooped by Mark Bayliss from Big Data
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The 147 Companies that Control Everything

The 147 Companies that Control Everything | NMR | Scoop.it
“ Visualizing the "super entity." Courtesy: New Scientist Three systems theorists at the Swiss Federal Institute of Technology in Zurich have taken a database listing 37 million companies and investors worldwide and analyzed all 43,060 transnational...”
Via Ed Stenson
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Rescooped by Mark Bayliss from Natural Products Chemistry Breaking News
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Proton Fingerprints Portray Molecular Structures: Enhanced Description of the 1H NMR Spectra of Small Molecules

Proton Fingerprints Portray Molecular Structures: Enhanced Description of the 1H NMR Spectra of Small Molecules | NMR | Scoop.it
The characteristic signals observed in NMR spectra encode essential information on the structure of small molecules. However, extracting all of this information from complex signal patterns is not trivial. This report demonstrates how computer-aided spectral analysis enables the complete interpretation of 1D 1H NMR data. The effectiveness of this approach is illustrated with a set of organic molecules, for which replicas of their 1H NMR spectra were generated. The potential impact of this methodology on organic chemistry research is discussed. José G. Napolitano†, David C. Lankin†, James B. McAlpine†, Matthias Niemitz‡, Samuli-Petrus Korhonen‡, Shao-Nong Chen†, and Guido F. PauliJ. Org. Chem., Article ASAPDOI: 10.1021/jo4011624Publication Date (Web): September 6, 2013
Via NatProdChem
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Rescooped by Mark Bayliss from Natural and Alternative Medical News
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Men's Use of Testosterone on the Rise

Men's Use of Testosterone on the Rise | NMR | Scoop.it
“ A new report shows that the number of older and middle-aged men prescribed the hormone has tripled since 2001, raising concerns about abuse and mistreatment.”
Via Phoenix Natural Medicine
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msCompare: a framework for quantitative analysis of label-free LC-MS data for comparative candidate biomarker studies.

Related Articles msCompare: a framework for quantitative analysis of label-free LC-MS data for comparative candidate biomarker studies. Mol Cell Proteomics.
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Rescooped by Mark Bayliss from Natural Products Chemistry Breaking News
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Accurate mass–time tag library for LC/MS-based metabolite profiling of medicinal plants

Accurate mass–time tag library for LC/MS-based metabolite profiling of medicinal plants | NMR | Scoop.it
We report the development and testing of an accurate mass–time (AMT) tag approach for the LC/MS-based identification of plant natural products (PNPs) in complex extracts. An AMT tag library was developed for approximately 500 PNPs with diverse chemical structures, detected in electrospray and atmospheric pressure chemical ionization modes (both positive and negative polarities). In addition, to enable peak annotations with high confidence, MS/MS spectra were acquired with three different fragmentation energies. The LC/MS and MS/MS data sets were integrated into online spectral search tools and repositories (Spektraris and MassBank), thus allowing users to interrogate their own data sets for the potential presence of PNPs. The utility of the AMT tag library approach is demonstrated by the detection and annotation of active principles in 27 different medicinal plant species with diverse chemical constituents. Daniel J. Cuthbertsona, 1,Sean R. Johnsona,Jasenka Piljac-Žegaraca, b,Julia Kappela, c,Sarah Schäfera, c,Matthias Wüstc,Raymond E.B. Ketchuma, 2,Rodney B. Croteaua,Joaquim V. Marquesa,Laurence B. Davina,Norman G. Lewisa,Megan Rolfd,Toni M. Kutchand,D. Doel Soejartoe, f,B. Markus Langea, , Phytochemistry, in Press
Via NatProdChem
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Rescooped by Mark Bayliss from COOL 3DPRINTING
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'Body on a chip' used for drug tests

'Body on a chip' used for drug tests | NMR | Scoop.it
A technology which puts miniature human organs made with a 3D printer on a microchip is being used to test new drugs and vaccines.Miniature human organs developed with a modified 3D printer are being used to test new vaccines in a lab in the US.The "body on a chip" project replicates human cells to print structures which mimic the functions of the heart, liver, lung and blood vessels.The organs are then placed on a microchip and connected with a blood substitute, allowing scientists to closely monitor specific treatments.The US Department of Defense has backed the new technology with $24m (£15m).Bioprinting, a form of 3D printing which, in effect, creates human tissue, is not new. Nor is the idea of culturing 3D human tissue on a microchip.
Continue reading the main story

Via Annie Theunissen
Mark Bayliss's insight:
The impact of this technology in routine pharmaceutical drug discovery would be enormous if it really works. It may even enable much better predictive models to be developed.
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Rescooped by Mark Bayliss from LC-MS-MS
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Are generics really the same as branded drugs? - Fortune Management

Are generics really the same as branded drugs? - Fortune Management | NMR | Scoop.it
“ Consumers are told that generics are just like their name-brand counterparts. More medical professionals are starting to say that's not the case.”
Via Pablo Irurzun
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Rescooped by Mark Bayliss from Amazing Science
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Maverick malaria vaccine achieves 100% protection using parasites from irradiated mosquitoes

Maverick malaria vaccine achieves 100% protection using parasites from irradiated mosquitoes | NMR | Scoop.it
A malaria vaccine has become the first to provide 100% protection against the disease, confounding critics and far surpassing any other experimental malaria vaccine tested. It will now be tested further in clinical trials in Africa. The results are important because they demonstrate for the first time the concept that a malaria vaccine can provide a high level of protection, says Anthony Fauci, director of the US National Institute of Allergy and Infectious Diseases in Bethesda. No effective malaria vaccine is available at present. The World Health Organization has set a target to develop a malaria vaccine with 80% efficacy by 2025, but until now, says Fauci, "we have not even gotten anywhere near that level of efficacy." Scientists had previously been sceptical of the vaccine because producing it required overcoming massive logistical hurdles. The vaccine — called PfSPZ because it is made from sporozoites (SPZ), a stage in the life cycle of the malarial parasitePlasmodium falciparum (Pf) — uses a weakened form of the whole parasite to invoke an immune response. In the phase I safety trial, reported today in Science, the six subjects given five doses intravenously were 100% protected from later challenge by bites of infectious mosquitoes, whereas five of six unvaccinated controls developed malaria — as did three of nine people given only four doses of the vaccine. PfSPZ was developed by Sanaria, a company based in Rockville, Maryland, and led by Stephen Hoffman, a veteran malaria researcher who also led the PfSPZ clinical trial. Most malaria-vaccine candidates are recombinant-subunit vaccines containing just a handful of parasite proteins, but Hoffman decided to test the whole-sporozoite vaccine on the basis of past experiments dating back to the 1970s showing that strong and long-lived protection could be obtained by exposing volunteers to thousands of bites from irradiated infected mosquitoes. That the vaccine works so well is a "pivotal success," says Stefan Kappe, a malaria researcher at the Seattle Biomedical Research Institute in Washington."The trial results constitute the most important advance in malaria vaccine development since the first demonstration of protection with radiation attenuated sporozoite immunization by mosquito bite in the 70s." But to make PfSPZ was challenging. Sanaria succeeded in raising mosquitoes in sterile conditions on an industrial scale, feeding them blood infected with the malaria parasite and then irradiating them to weaken the parasite so that it can still infect people but not cause disease. Billions of parasites were then harvested from the mosquitoes' salivary glands, purified and cryopreserved. Many researchers were highly sceptical that sporozoites could be mass-produced in a way that passed the strict quality and safety standards needed for human medicines, notes Fauci. "To my amazement, Hoffman did it," he adds.
Via Dr. Stefan Gruenwald
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Rescooped by Mark Bayliss from Sustain Our Earth
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This amazing little patch keeps mosquitoes away without any toxic chemicals

This amazing little patch keeps mosquitoes away without any toxic chemicals | NMR | Scoop.it
“ It uses non-toxic compounds to screw with mosquitoes' sense of smell.”
Via SustainOurEarth
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Rescooped by Mark Bayliss from Mass spectrometry
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Guidelines for reporting quantitative mass spectrometry based experiments in proteomics

Guidelines for reporting quantitative mass spectrometry based experiments in proteomics | NMR | Scoop.it

Via Edwin De Pauw
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Rescooped by Mark Bayliss from Prostate Health- Advances, Knowledge, Integrative & Holistic Treatments
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9 Tips: Prevent Prostate Cancer

9 Tips: Prevent Prostate Cancer | NMR | Scoop.it
“ Research has shown that diet can play a role in preventing prostate cancer. Here are some important dietary and lifestyle tips to reduce your risk of prostate cancer. (Research has shown that diet can play a role in preventing #prostatecancer.”
Via 27 Prostate Health
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Rescooped by Mark Bayliss from Health & Life Extension
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Research yields first detailed view of morphing Parkinson's protein

Research yields first detailed view of morphing Parkinson's protein | NMR | Scoop.it
Researchers have taken detailed images and measurements of the morphing structure of a brain protein thought to play a role in Parkinson's disease, information that could aid the development of medications to treat the condition.
Via Tonya Scholz
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Rescooped by Mark Bayliss from Natural Products Chemistry Breaking News
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Mass spectrometry and NMR spectroscopy: modern high-end detectors for high resolution separation techniques – state of the art in natural product HPLC-MS, HPLC-NMR, and CE-MS hyphenations

Mass spectrometry and NMR spectroscopy: modern high-end detectors for high resolution separation techniques – state of the art in natural product HPLC-MS, HPLC-NMR, and CE-MS hyphenations | NMR | Scoop.it
Current natural product research is unthinkable without the use of high resolution separation techniques as high performance liquid chromatography or capillary electrophoresis (HPLC or CE respectively) combined with mass spectrometers (MS) or nuclear magnetic resonance (NMR) spectrometers. These hyphenated instrumental analysis platforms (CE-MS, HPLC-MS or HPLC-NMR) are valuable tools for natural product de novo identification, as well as the authentication, distribution, and quantification of constituents in biogenic raw materials, natural medicines and biological materials obtained from model organisms, animals and humans. Moreover, metabolic profiling and metabolic fingerprinting applications can be addressed as well as pharmacodynamic and pharmacokinetic issues. This review provides an overview of latest technological developments, discusses the assets and drawbacks of the available hyphenation techniques, and describes typical analytical workflows. Christoph Seger,ab Sonja Sturma and Hermann Stuppner Nat. Prod. Rep., 2013, Advance Article DOI: 10.1039/C3NP70015A
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Rescooped by Mark Bayliss from Design Arena
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Present.me: Make a slideshow with your powerpoint & web cam

Present.me: Make a slideshow with your powerpoint & web cam | NMR | Scoop.it
Present.me is a video presentation platform. Share all your presentation content in one place. Slides, slides and audio, slides and video or just video. No software to install, all you need is your slides, a webcam and YOU! Discover for yourself......how thousands of people are sharing their ideas every day... >> It's in the cloud... >> Easy to Import... >> Easy to record... >> Easy to share... >> Easy to edit... >> It's mobile... >> More than slides... >> Private and secure... >> Analytics & Engagement... "Present.me is a brilliant way for us to talk to our clients" - Partner, Big Four Accountants
Via Mhd.Shadi Khudr
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