Neuroscience_technics
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Neuroscience_technics
Neuroscience requires a wide range of technologies
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Scooped by Julien Hering, PhD
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Selective and regulated gene expression in murine Purkinje cells by in utero electroporation

Selective and regulated gene expression in murine Purkinje cells by in utero electroporation | Neuroscience_technics | Scoop.it

A new in utero electroporation (IUE) protocol has been developed to deliver genes preferentially into cerebellar Purkinje cells. IUE did not alter the physiological characteristics or normal synaptic plasticity of Purkinje cells. IUE allowed selective, effective, and temporally regulated expression of multiple foreign genes in Purkinje cells in vivo. (...) by Nishiyama J et al.European Journal of Neuroscience, Volume 36Issue 7pages 2867–2876October 2012

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Scooped by Julien Hering, PhD
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Viral transduction of the neonatal brain delivers controllable genetic mosaicism for visualising and manipulating neuronal circuits in vivo

Viral transduction of the neonatal brain delivers controllable genetic mosaicism for visualising and manipulating neuronal circuits in vivo | Neuroscience_technics | Scoop.it

Freehand injection of recombinant AAV into the neonatal mouse brain offers a fast and easy way to attain widespread genetic manipulation of neurons throughout the brain. Rapid onset and year-long persistence of viral expression permits study of both critical periods and aging. Viral titer can be used to control mosaicism, and multiple viruses can be co-injected for bigenic expression. The technique’s simplicity and the availability of viral reagents should facilitate a range of experiments. (...) - by Ji-Yoen Kim et al.European Journal of Neuroscience, Volume 37Issue 8pages 1203–1220April 2013

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