Viral transduction of the neonatal brain delivers controllable genetic mosaicism for visualising and manipulating neuronal circuits in vivo | Neuroscience_technics |

Freehand injection of recombinant AAV into the neonatal mouse brain offers a fast and easy way to attain widespread genetic manipulation of neurons throughout the brain. Rapid onset and year-long persistence of viral expression permits study of both critical periods and aging. Viral titer can be used to control mosaicism, and multiple viruses can be co-injected for bigenic expression. The technique’s simplicity and the availability of viral reagents should facilitate a range of experiments. (...) - by Ji-Yoen Kim et al.European Journal of Neuroscience, Volume 37Issue 8pages 1203–1220April 2013