microRNA research
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Rescooped by Emily Goodall from DNA and RNA Research
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Gone FISHing for Cancer microRNAs

Gone FISHing for Cancer microRNAs | microRNA research | Scoop.it

By fine-tuning the design of fluorescent probes and RNA fixation techniques, researchers have developed a way to detect and quantify microRNA molecules in tumor biopsy samples. The new fluorescence in situ hybridization (FISH) method allows the identification of tumor types that contain unique combinations of microRNAs. And unlike previous methods, the approach allows the simultaneous detection of multiple microRNAs while leaving the biopsy sample intact.


Via Integrated DNA Technologies
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Felix J. Tapia's curator insight, June 6, 2013 12:53 PM

Important for Mexico talk

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Identifying a blood based microRNA fingerprint for MND | Research | MND Association

Identifying a blood based microRNA fingerprint for MND | Research | MND Association | microRNA research | Scoop.it
Identifying a blood based microRNA fingerprint for MND
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MicroRNA-206 Delays ALS Progression and Promotes Regeneration of Neuromuscular Synapses in Mice

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Prognostic RNA Markers Identified In A Common Form Of Breast Cancer

Prognostic RNA Markers Identified In A Common Form Of Breast Cancer | microRNA research | Scoop.it

"A Big-Data analysis that integrates three large sets of genomic data available through The Cancer Genome Atlas has identified 37 RNA molecules that might predict survival in patients with the most common form of breast cancer.

The study by researchers at the Ohio State University Comprehensive Cancer Center - Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC - James) initially analyzed messenger RNA (mRNA) and microRNA expression, DNA methylation data and clinical findings for 466 patients with invasive ductal carcinoma, the most common type of breast cancer."


Via Susan Zager
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Susan Zager's curator insight, May 1, 2013 4:08 PM

These genes can be candidates for early blood detection.


"Key points related to the study's findings include:

  • The identified RNA signature might predict response to treatment, as well as being prognostic;
  • DNA methylation was used to confirm the association between mRNA expression and overall survival;
  • The signature includes mutations in PIK3CA and its pathway, indicating that the PIK3CA/AKT2/PTEN axis is an important and independent cofactor in prognosis;
  • The prognostic value of the integrated signature was highest in early stage I and II breast cancers, making this a potentially valuable biomarker signature in clinical practice."


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Involvement of MicroRNA in Copper Deficiency-Induced Repression of Chloroplastic CuZn-Superoxide Dismutase Genes in the Moss Physcomitrella patens

Involvement of MicroRNA in Copper Deficiency-Induced Repression of Chloroplastic CuZn-Superoxide Dismutase Genes in the Moss Physcomitrella patens | microRNA research | Scoop.it

Superoxide dismutases (SODs) are metallo-enzymes that catalyze the dismutation of superoxide radicals. In Arabidopsis thaliana, the expression of CuZn-SOD in both the chloroplast and cytosol was reported to be down-regulated by microRNA398 (miR398) during growth on low copper. The moss Physcomitrella patens contains chloroplastic and cytosolic CuZn-SOD genes, but lacks miR398. From analysis of P. patens microRNA, miR1073 was predicted to target CuZn-SOD mRNAs. We noticed that two chloroplastic CuZn-SOD genes contain the miR1073 target sequence in the 3′ UTR region; however, cytosolic isozyme genes lack this sequence. In this study, we investigated the involvement of miR1073 in the expression of CuZn-SOD genes in P. patens. When protonemata of P. patens were cultured on a copper-depleted medium, SOD activity and mRNA levels of chloroplastic CuZn-SODs were decreased markedly. In contrast, cytosolic CuZn-SODs showed little or no change in mRNA levels or SOD activity. The precursor transcript and the mature form of miR1073 were induced by copper deficiency. The chloroplastic CuZn-SOD (PpCSD1) mRNA was cleaved at the miR1073 target site under copper deficiency. These results suggest that miR1073 is involved in the down-regulation of PpCSD1 expression.

In addition to PpCSD1 mRNA, antisense RNAs of PpCSD1 were also detected under normal conditions; however, under copper deficiency, they were cleaved within the ORF region. The cleavage of sense PpCSD1 mRNA was also detected within the ORF region. Although only miR1073 exists in the database, it is presumed that RNA cleavage, other than that mediated by miR1073, is involved in the regulation of PpCSD1 expression.


Via Jean-Pierre Zryd
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Age-Dependent MicroRNA Control of Synaptic Plasticity in 22q11 Deletion Syndrome and Schizophrenia

Age-Dependent MicroRNA Control of Synaptic Plasticity in 22q11 Deletion Syndrome and Schizophrenia | microRNA research | Scoop.it

The 22q11 deletion syndrome (22q11DS) is characterized by multiple physical and psychiatric abnormalities and is caused by the hemizygous deletion of a 1.5–3 Mb region of chromosome 22. It constitutes one of the strongest known genetic risks for schizophrenia; schizophrenia arises in as many as 30% of patients with 22q11DS during adolescence or early adulthood. A mouse model of 22q11DS displays an age-dependent increase in hippocampal long-term potentiation (LTP), a form of synaptic plasticity underlying learning and memory. The sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA2), which is responsible for loading Ca2+ into the endoplasmic reticulum (ER), is elevated in this mouse model. The resulting increase in ER Ca2+ load leads to enhanced neurotransmitter release and increased LTP. However, the mechanism by which the 22q11 microdeletion leads to SERCA2 overexpression and LTP increase has not been determined. Screening of multiple mutant mouse lines revealed that haploinsufficiency of Dgcr8, a microRNA (miRNA) biogenesis gene in the 22q11DS disease-critical region, causes age-dependent, synaptic SERCA2 overexpression and increased LTP. We found that miR-25 and miR-185, regulators of SERCA2, are depleted in mouse models of 22q11DS. Restoration of these miRNAs to presynaptic neurons rescues LTP in Dgcr8+/− mice. Finally, we show that SERCA2 is elevated in the brains of patients with schizophrenia, providing a link between mouse model findings and the human disease. We conclude that miRNA-dependent SERCA2 dysregulation is a pathogenic event in 22q11DS and schizophrenia. - by Earls LR et al., The Journal of Neuroscience, 10 October 2012, 32(41): 14132-14144


Via Julien Hering, PhD
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Research: MicroRNA in the blood change with different type of MS

Research: MicroRNA in the blood change with different type of MS | microRNA research | Scoop.it
OBJECTIVE: MicroRNA (miRNAs) are single stranded, small non-coding RNAs that regulate gene expression. Because they are stable in serum, they are being developed as biomarkers for cancer and other diseases.
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