A decade ago this week, scientists announced that they had completed the Human Genome Project. Eric D. Green, director of the National Human Genome Research Institute, discussed what that accomplishment meant and what is coming next.
William Gibson's popular science fiction tale "Johnny Mnemonic" foresaw sensitive information being carried by microchips in the brain by 2021. A team of American neuroscientists could be making this fantasy world a reality.
This is the official website of STELARC. Stelarc is a performance artist who has visually probed and acoustically amplified his body. Between 1976-1988 he completed 25 body suspension performances with hooks into his skin.
In this study, we utilized a cell-penetrating peptide-based system for ZFN and TALEN delivery. Functional TAT-ZFN and TAT-TALEN proteins were generated by fusing the cell-penetrating TAT peptide to ZFN and TALEN, respectively. However, TAT-ZFN was difficult to purify in quantities sufficient for analysis in cell culture. Purified TAT-TALEN was able to penetrate cells and disrupt the gene encoding endogenous human chemokine (C-C motif) receptor 5 (CCR5, a co-receptor for HIV-1 entry into cells). Hypothermic treatment greatly enhanced the TAT-TALEN-mediated gene disruption efficiency. A 5% modification rate was observed in human induced pluripotent stem cells (hiPSCs) treated with TAT-TALEN as measured by the Surveyor assay.
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