Light Sheet Microsopy
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Wikipedia Entry

Light sheet fluorescence microscopy (LSFM) or Selective/Single plane illumination microscopy (SPIM) is a fluorescence microscopy technique with an intermediate optical resolution, but good sectioning capabilities. In contrast to Epi fluorescence microscopy only a thin slice (usually a few hundret nanometers to a few micrometers) of the sample is illuminated perpendicularyl to the direction of observation. For illumination a laser lightsheet is used, i.e. a laserbeam which is focused only in one direction (e.g. using a cylindrical lens). A second method uses a circular beam scanned in one direction to create the lightsheet. As only the actually observed section is illuminated, this method reduces the photodamage and stress induced on a living sample. Also the good sectioning capability reduces the background signal and thus creates images with higher contrast, comparable to confocal microscopy.

This method is used in cellular biology[1] and for microscopy of whole living creatures, such as embryos.[2] It is now often used for the longtime observation of embryonal development in different model organisms.

LSFM was developed during the first years of the 21. century[3][4] and introduced an illumination scheme into fluorescence microscopy, which has already been used successfully for dark field microscopy under the name ultra microscopy.[5]

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Zeiss General Product Website

Zeiss General Product Website | Light Sheet Microsopy | Scoop.it
Use Light Sheet Microscopy for imaging applications with large living samples, including fluorescent labeled specimens. The Lightsheet Z.1 is also the appropriate LSFM imaging system for fluorescence protein labeling.
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Confocal light-sheet microscopy is sharper way to view the brain's neural network - Laser Focus World

Confocal light-sheet microscopy is sharper way to view the brain's neural network - Laser Focus World | Light Sheet Microsopy | Scoop.it
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Non-Commercial Reference

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ZEISS Microscopy Online Campus | Light Sheet Microscopy References

ZEISS Microscopy Online Campus | Light Sheet Microscopy References | Light Sheet Microsopy | Scoop.it
The references listed in this section point to review articles that should provide the starting point for a thorough understanding of light sheet microscopy.
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Zeiss Product Information Download Site

Zeiss Product Information Download Site | Light Sheet Microsopy | Scoop.it
Use Light Sheet Microscopy for imaging applications with large living samples, including fluorescent labeled specimens. The Lightsheet Z.1 is also the appropriate LSFM imaging system for fluorescence protein labeling.
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Here you can download brochures, specifications and images

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Optical Sectioning using Light Sheet Microscopy: | AAAS Webinars

Optical Sectioning using Light Sheet Microscopy: | AAAS Webinars | Light Sheet Microsopy | Scoop.it
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You can download the transcript from this webinar

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