Genome Engineering
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Novel Injection Could Reduce Heart Attack Risk by 40 to 90 Percent, Harvard ... - Nature World News

Novel Injection Could Reduce Heart Attack Risk by 40 to 90 Percent, Harvard ... - Nature World News | Genome Engineering | Scoop.it
Nature World News
Novel Injection Could Reduce Heart Attack Risk by 40 to 90 Percent, Harvard ...
Nature World News
Researchers then tried to see if they could permanently change the genome to make the "good defects" in the PCSK9 gene.
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Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs | Genome Engineering | Scoop.it
PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world. (Thinking about using #CRISPR / #cas9?
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Targeting cells with single vectors using multiple-feature Boolean logic : Nature Methods : Nature Publishing Group

Targeting cells with single vectors using multiple-feature Boolean logic : Nature Methods : Nature Publishing Group | Genome Engineering | Scoop.it
This paper describes a single-vector strategy for flexible intersectional expression of genetically encoded reporters and tools in precisely defined cell types in the mouse.
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CRISPR technology for gene therapy : Nature Medicine : Nature Publishing Group

CRISPR technology for gene therapy : Nature Medicine : Nature Publishing Group | Genome Engineering | Scoop.it

This article comments on the recent study published in Nature Biotechnology on in vivo gene therapy in the mouse using the CRISPR/Cas9 system.

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Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification : Nature Biotechnology : Nature Publishing Group

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification : Nature Biotechnology : Nature Publishing Group | Genome Engineering | Scoop.it
A fusion of the FokI nuclease and a catalytically inactive Cas9 is a highly specific genome editing tool.
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Molecular Therapy — Nucleic Acids - Pol III Promoters to Express Small RNAs: Delineation of Transcription Initiation

Molecular Therapy — Nucleic Acids - Pol III Promoters to Express Small RNAs: Delineation of Transcription Initiation | Genome Engineering | Scoop.it

This paper characterizes the RNA Pol III promoter used commonly to express gRNAs and shRNAs.  Transcription can be initiated accurately with an A or G so long as the promoter is left unchanged.

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Horizon Expands CRISPR-Cas9 Portfolio with Broad Institute Licensing Deal | GenomeWeb Daily News | GenomeWeb

Horizon Expands CRISPR-Cas9 Portfolio with Broad Institute Licensing Deal | GenomeWeb Daily News | GenomeWeb | Genome Engineering | Scoop.it
  (Horizon Expands CRISPR-Cas9 Portfolio with Broad Institute Licensing Deal: NEW YORK (GenomeWeb) – Horizon Disc... http://t.co/UgjFJ8ptqb)
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Unravelling the structural and mechanistic basis of CRISPR-Cas systems : Nature Reviews Microbiology : Nature Publishing Group

Unravelling the structural and mechanistic basis of CRISPR-Cas systems : Nature Reviews Microbiology : Nature Publishing Group | Genome Engineering | Scoop.it
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Reversible protein inactivation by optogenetic trapping in cells : Nature Methods : Nature Publishing Group

Reversible protein inactivation by optogenetic trapping in cells : Nature Methods : Nature Publishing Group | Genome Engineering | Scoop.it
Light-activated reversible inhibition by assembled trap (LARIAT) is a versatile optogenetic method for inactivating proteins, including GFP fusions, in living cells by conditional clustering at high spatiotemporal resolution.
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Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing : Nature Biotechnology : Nature Publishing Group

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing : Nature Biotechnology : Nature Publishing Group | Genome Engineering | Scoop.it
More specific RNA-guided genome editing
A fusion of the FokI nuclease and a catalytically inactive Cas9 is a highly specific genome editing tool.
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Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells : Nature Biotechnology : Nature Publishing Group

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells : Nature Biotechnology : Nature Publishing Group | Genome Engineering | Scoop.it
Genome-wide analysis of Cas9-DNA interactions in mammalian cells shows widespread binding but low levels of cleavage.
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CRISPR transcriptional repression devices and layered circuits in mammalian cells : Nature Methods : Nature Publishing Group

CRISPR transcriptional repression devices and layered circuits in mammalian cells : Nature Methods : Nature Publishing Group | Genome Engineering | Scoop.it
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9-based transcriptional repressors can be easily engineered to give rise to a large library of orthogonal devices for complex circuits in mammalian cells.
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