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Rescooped by Marisa Irigoyen from Awesome Science That I Like
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Targeted Degradation of Abscisic Acid Receptors Is Mediated by the Ubiquitin Ligase Substrate Adaptor DDA1 in Arabidopsis

Targeted Degradation of Abscisic Acid Receptors Is Mediated by the Ubiquitin Ligase Substrate Adaptor DDA1 in Arabidopsis | Irigoyen | Scoop.it

CULLIN4-RING E3 ubiquitin ligases (CRL4s) regulate key developmental and stress responses in eukaryotes. Studies in both
animals and plants have led to the identification of many CRL4 targets as well as specific regulatory mechanisms that modulate
their function. The latter involve COP10-DET1-DDB1 (CDD)–related complexes, which have been proposed to facilitate target
recognition by CRL4, although the molecular basis for this activity remains largely unknown. Here, we provide evidence that
Arabidopsis thaliana DET1-, DDB1-ASSOCIATED1 (DDA1), as part of the CDD complex, provides substrate specificity for CRL4
by interacting with ubiquitination targets. Thus, we show that DDA1 binds to the abscisic acid (ABA) receptor PYL8, as well as
PYL4 and PYL9, in vivo and facilitates its proteasomal degradation. Accordingly, we found that DDA1 negatively regulates ABAmediated
developmental responses, including inhibition of seed germination, seedling establishment, and root growth. All other
CDD components displayed a similar regulatory function, although they did not directly interact with PYL8. Interestingly, DDA1-
mediated destabilization of PYL8 is counteracted by ABA, which protects PYL8 by limiting its polyubiquitination. Altogether, our
data establish a function for DDA1 as a substrate receptor for CRL4-CDD complexes and uncover a mechanism for the
desensitization of ABA signaling based on the regulation of ABA receptor stability.


Via Jorge Lozano-Juste
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Jorge Lozano-Juste's curator insight, February 26, 2:00 PM

Ub-Proteasome hits ABA receptors

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Transmitting Plant Viruses Using Whiteflies

To watch this video on jove.com, click here: http://www.jove.com/video/4332?utm_source=youtube Improving our understanding of and our ability to manage many ...
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