High Content Screening
113 views | +0 today
Follow
Your new post is loading...
Your new post is loading...
Scooped by High Content Review
Scoop.it!

GEN | Insight & Intelligence™: The Renaissance of Phenotypic Research

GEN | Insight & Intelligence™: The Renaissance of Phenotypic Research | High Content Screening | Scoop.it
Serendipitous pharmaceutical discovery is making a comeback through high-content screening.
more...
No comment yet.
Scooped by High Content Review
Scoop.it!

GEN | Magazine Articles: Magazine Preview: High-Content Analysis—The “Just Right” Solution

GEN | Magazine Articles: Magazine Preview: High-Content Analysis—The “Just Right” Solution | High Content Screening | Scoop.it
High-content analysis is not just for cell biologists. Better software, hardware, and informatics tools make it increasingly relevant to drug developers.
more...
No comment yet.
Rescooped by High Content Review from Virology and Bioinformatics from Virology.ca
Scoop.it!

Single-cell technologies for monitoring immune systems : Nature Immunology

Single-cell technologies for monitoring immune systems : Nature Immunology | High Content Screening | Scoop.it

The complex heterogeneity of cells, and their interconnectedness with each other, are major challenges to identifying clinically relevant measurements that reflect the state and capability of the immune system. Highly multiplexed, single-cell technologies may be critical for identifying correlates of disease or immunological interventions as well as for elucidating the underlying mechanisms of immunity. Here we review limitations of bulk measurements and explore advances in single-cell technologies that overcome these problems by expanding the depth and breadth of functional and phenotypic analysis in space and time. The geometric increases in complexity of data make formidable hurdles for exploring, analyzing and presenting results. We summarize recent approaches to making such computations tractable and discuss challenges for integrating heterogeneous data obtained using these single-cell technologies.


Via burkesquires
more...
No comment yet.
Rescooped by High Content Review from Drug Discovery
Scoop.it!

High-Throughput Screening Normalized to Biological Response

The process of conducting cell-based phenotypic screens can result in data sets from small libraries or portions of large libraries, making accurate hit picking from multiple data sets important for efficient drug discovery. Here, we describe a screen design and data analysis approach that allow for normalization not only between quadrants and plates but also between screens or batches in a robust, quantitative fashion, enabling hit selection from multiple data sets. We independently screened the MicroSource Spectrum and NCI Diversity Set II libraries using a cell-based phenotypic high-throughput screening (HTS) assay that uses an interferon-stimulated response element (ISRE)–driven luciferase-reporter assay to identify interferon (IFN) signal enhancers. Inclusion of a per-plate, per-quadrant IFN dose-response standard curve enabled conversion of ISRE activity to effective IFN concentrations. We identified 45 hits based on a combined z score ≥2.5 from the two libraries, and 25 of 35 available hits were validated in a compound concentration-response assay when tested using fresh compound. The results provide a basis for further analysis of chemical structure in relation to biological function. Together, the results establish an HTS method that can be extended to screening for any class of compounds that influence a quantifiable biological response for which a standard is available.


Via CYTOO
more...
No comment yet.
Scooped by High Content Review
Scoop.it!

Terry Hébert studies cellular signaling using high content imaging and phenotypic analyses

Terry Hébert explains how his research group at McGill University is using PerkinElmer's Operetta® High Content Imaging System for phenotypic analyses to ans...
more...
No comment yet.
Scooped by High Content Review
Scoop.it!

Brian Reid: Using high content imaging & phenotypic analyses to investigate heart failure therapies

Brian Reid discusses how his research group at the University of Colorado is using PerkinElmer's Operetta® High Content Imaging System for phenotypic studies...
more...
No comment yet.
Scooped by High Content Review
Scoop.it!

SBI2 West Coast Regional Meeting - January 23rd, 2014 | High Content Review

SBI2 West Coast Regional Meeting - January 23rd, 2014 | High Content Review | High Content Screening | Scoop.it
more...
No comment yet.
Scooped by High Content Review
Scoop.it!

HCA On-line Q&A at Thermo Fisher Scientific | High Content Review

HCA On-line Q&A at Thermo Fisher Scientific | High Content Review | High Content Screening | Scoop.it
High Content Review's insight:

Have you ever needed some quick advice or had a question about Thermo Fisher Scientific Cellular Imaging and Analysis products, high content technology, cell biology, assay development or imaging?  Well, now you can submit a question from a new portal on their website dedicated to HCS education.

more...
No comment yet.
Scooped by High Content Review
Scoop.it!

Introducing the New Opera Phenix High Content Screening System For Phenotypic Screening and Complex Disease Models | High Content Review

Introducing the New Opera Phenix High Content Screening System For Phenotypic Screening and Complex Disease Models | High Content Review | High Content Screening | Scoop.it
more...
No comment yet.
Rescooped by High Content Review from High Content Screening
Scoop.it!

Cell-Based Fuzzy Metrics Enhance High-Content Screening (HCS) Assay Robustness

High-content screening (HCS) allows the exploration of complex cellular phenotypes by automated microscopy and is increasingly being adopted for small interfering RNA genomic screening and phenotypic drug discovery. We introduce a series of cell-based evaluation metrics that have been implemented and validated in a mono-parametric HCS for regulators of the membrane trafficking protein caveolin 1 (CAV1) and have also proved useful for the development of a multiparametric phenotypic HCS for regulators of cytoskeletal reorganization. Imaging metrics evaluate imaging quality such as staining and focus, whereas cell biology metrics are fuzzy logic–based evaluators describing complex biological parameters such as sparseness, confluency, and spreading. The evaluation metrics were implemented in a data-mining pipeline, which first filters out cells that do not pass a quality criterion based on imaging metrics and then uses cell biology metrics to stratify cell samples to allow further analysis of homogeneous cell populations. Use of these metrics significantly improved the robustness of the monoparametric assay tested, as revealed by an increase in Z′ factor, Kolmogorov-Smirnov distance, and strict standard mean difference. Cell biology evaluation metrics were also implemented in a novel supervised learning classification method that combines them with phenotypic features in a statistical model that exceeded conventional classification methods, thus improving multiparametric phenotypic assay sensitivity.


Via CYTOO
more...
No comment yet.
Rescooped by High Content Review from Natural Products Chemistry Breaking News
Scoop.it!

Approaches to Protozoan Drug Discovery: Phenotypic Screening

Approaches to Protozoan Drug Discovery: Phenotypic Screening | High Content Screening | Scoop.it

Determining the activity of a compound and the potential impact on a diseased state is frequently undertaken using phenotypic or target-based approaches. Phenotypic screens have the advantage of the whole organism being exposed to the compound and thus all the targets and biological pathways associated with it. Cell penetration and access to targets in their “natural” environment are taken into account. Unless utilizing a genetically modified organism with an additional target associated indicator, elucidation of specific target(s) of active compounds is necessary. Target discovery is desirable to allow development of chemical entities based upon knowledge of the target structure. Phenotypic drug discovery has successfully identified new molecular entities for neglected protozoan disease research. In this perspective, the phenotypic approaches used to identify chemical entities for drug discovery and for use as tools against the parasites Plasmodium falciparum, Trypanosoma brucei brucei, and Trypanosoma cruzi will be outlined.

 

Melissa L. Sykes and Vicky M. Avery*Discovery Biology, Eskitis Institute for Drug Discovery, Griffith University, Nathan, Queensland, Australia

 

J. Med. Chem., Article ASAP

DOI: 10.1021/jm4004279


Via NatProdChem
more...
No comment yet.
Scooped by High Content Review
Scoop.it!

Paul Antony studies cellular phenotypes using HCS

Dr. Paul Antony, Luxembourg Centre for Systems Biomedicine, talks about how his research group is studying cellular phenotypes using the Opera® High Content ...
more...
No comment yet.
Scooped by High Content Review
Scoop.it!

Lance Davidow investigates neurodegenerative diseases using high content imaging and analysis

Lance Davidow talks about how his group at Harvard Stem Cell Institute is studying the role of biochemical pathways in various neurodegenerative diseases. He...
more...
No comment yet.
Scooped by High Content Review
Scoop.it!

NEW MultiCyt™ Qbeads Plexscreen on iQue Screener - SelectScience.net (press release)

NEW MultiCyt™ Qbeads Plexscreen on iQue Screener - SelectScience.net (press release) | High Content Screening | Scoop.it
NEW MultiCyt™ Qbeads Plexscreen on iQue Screener
SelectScience.net (press release)
Visit IntelliCyt Corporation at booth 1341 and learn how to revolutionize your phenotypic screening with the iQue™ Screener platform.
more...
No comment yet.