An experiment was conducted to study the effect of the fruit shelf-life and the
concentrations of carbon source on somatic embryogenesis via callogenesis of Hevea
brasiliensis. Fruits harvested were stored at 15°C during 1, 5, 7, 12 and 15 days and the
inner integuments obtained from seed were used as explants. The experiments were
performed under carbon source treatments with three concentrations for glucose and five
concentrations for sucrose. Under these conditions, the percentage of explants forming
calli was better during the first week of fruit preservation regardless of the carbon source
but at high concentration. However, beyond 7 days of fruits shelf-life, sucrose is best to
induce callus unlike glucose but with high concentration. The best rate of embryogenic
calli was also obtained with sucrose. The percentages of callogenic explants and
embryogenic calli have decreased sharply with the shelf-life of fruit at 15°C. So, to
maintain an embryogenic potential of explants in situations of long-term conservation of
fruits, sucrose can be used at 234 mM of concentration or default at 111 mM sucrose.
These sucrose concentrations are conducive to induce embryogenic calli with explants
coming to rubber fruits after a long time of preservation.