Hevea brasiliensis
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MY2012000014 A METHOD FOR REGULATING BIOSYNTHESIS OF MONOLIGNOLS

MY2012000014 A METHOD FOR REGULATING BIOSYNTHESIS OF MONOLIGNOLS | Hevea brasiliensis | Scoop.it
(EN)A method for reducing the synthesis of monolignols in cytoplasm of the plant of Hevea brasiliensis, comprising the step of down-regulating expression of a nucleic acid template containing at least one nucleotide sequence set forth in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 1 1 or SEQ ID NO. 12 in at least one cell. Also claimed are nucleotide sequences encoding phenylalanine ammonia-lyase 1 (PALI), phenylalanine ammonia-lyase 2 (PAL2), cinnamate 4-hydroxylase (CH4), coumarate 3-hydroxylase 1 (C3H1), coumarate 3-hydroxylase 2 (C3H2), caffeic acid/5-hydroxyconiferaldehyde O-methyltransferase (COMT), Hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl transferase (HCT), ferulate 5-hydroxylase (F5H), 4-coumarate CoA ligase (4CL), 4-coumarate caffeoyl-CoA O-methyltransferase (CCoAOMT), 4-coumarate cinnamoyl coenzyme A reductase (CCRl), and 4-coumarate cinnamyl alcohol dehydrogenase (CAD) enzymes, all from Hevea brasiliensis.
(FR)La présente invention a pour objet un procédé pour la réduction de la synthèse de monolignols dans le cytoplasme de la plante d'Hevea brasiliensis, comprenant l'étape de régulation à la baisse de l'expression d'une matrice d'acide nucléique contenant au moins une séquence de nucléotides présentée dans SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11 ou SEQ ID NO. 12 dans au moins une cellule. La présente invention concerne également des séquences de nucléotides codant les enzymes phénylalanine ammoniac-lyase 1 (PAL1), phénylalanine ammoniac-lyase 2 (PAL2), cinnamate 4-hydroxylase (CH4), coumarate 3-hydroxylase 1 (C3H1), coumarate 3-hydroxylase 2 (C3H2), acide caféique/5-hydroxyconiféraldéhyde O-méthyltransférase (COMT), hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl transférase (HCT), férulate 5-hydroxylase (F5H), 4-coumarate CoA ligase (4CL), 4-coumarate cafféoyl-CoA O-méthyltransférase (CCoAOMT), 4-coumarate cinnamoyl coenzyme A réductase (CCRl), et 4-coumarate alcool cinnamylique déshydrogénase (CAD), toutes issues d'Hevea brasiliensis.

 

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Draft genome sequence of the rubber tree Hevea brasiliensis - 7thSpace Interactive (press release)

Draft genome sequence of the rubber tree Hevea brasiliensis 7thSpace Interactive (press release) Conclusions: The knowledge gained from this genome sequence will aid in the future development of high-yielding clones to keep up with the ever...
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Responses of rubber tree clone RRIM 600 to NPK fertilization on red yellow podzolic soil

Bataglia, Ondino Cleante; Dos Santos, Wagner Rodrigues; Igue, Toshio; De Souza Goncalves, Paulo, 1998: Responses of rubber tree clone RRIM 600 to NPK fertilization on red yellow podzolic soil (Responses of rubber tree clone RRIM 600 to NPK fertilization...
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De novo transcriptome analysis of Hevea brasiliensis tissues by RNA-seq and screening for molecular markers

The rubber tree, Hevea brasiliensis, is a species native to the Brazilian Amazon region and it supplies almost all the world's natural rubber, a strategic raw material for a variety of products. One of the major challenges for developing rubber tree plantations is adapting the plant to biotic and abiotic stress. Transcriptome analysis is one of the main approaches for identifying the complete set of active genes in a cell or tissue for a specific developmental stage or physiological condition.
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Background

The rubber tree, Hevea brasiliensis, is a species native to the Brazilian Amazon region and it supplies almost all the world's natural rubber, a strategic raw material for a variety of products. One of the major challenges for developing rubber tree plantations is adapting the plant to biotic and abiotic stress. Transcriptome analysis is one of the main approaches for identifying the complete set of active genes in a cell or tissue for a specific developmental stage or physiological condition.

Results

Here, we report on the sequencing, assembling, annotation and screening for molecular markers from a pool of H. brasiliensis tissues. A total of 17,166 contigs were successfully annotated. Then, 2,191 Single Nucleotide Variation (SNV) and 1.397 Simple Sequence Repeat (SSR) loci were discriminated from the sequences. From 306 putative, mainly non-synonymous SNVs located in CDS sequences, 191 were checked for their ability to characterize 23 Hevea genotypes by an allele-specific amplification technology. For 172 (90%), the nucleotide variation at the predicted genomic location was confirmed, thus validating the different steps from sequencing to the in silico detection of the SNVs.

Conclusions

This is the first study of the H. brasiliensis transcriptome, covering a wide range of tissues and organs, leading to the production of the first developed SNP markers. This process could be amplified to a larger set of in silico detected SNVs in expressed genes in order to increase the marker density in available and future genetic maps. The results obtained in this study will contribute to the H. brasiliensis genetic breeding program focused on improving of disease resistance and latex yield.

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Phosphate Solubilizing Bacteria and Fungi Isolated from Rubber (Hevea brasiliensis) Root Rhizosphere, Their Biofilm Formation and Phosphate Solubilizing Abilities | Hettiarachchi

Phosphate Solubilizing Bacteria and Fungi Isolated from Rubber (Hevea brasiliensis) Root Rhizosphere, Their Biofilm Formation and Phosphate Solubilizing Abilities
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Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript

Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript - up-to-the-minute news and headlines.
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(IUCr) Structural analysis of the endogenous glycoallergen Hev b 2 ...

Endogenous glycosylated Hev b 2 (endo- [beta] -1,3-glucanase) from Hevea brasiliensis is an important latex allergen that is recognized by IgE antibodies from patients who suffer from latex allergy. The carbohydrate moieties of Hev b 2 ...
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Rubber farmers in Mappi struggle to cope with falling - Jakarta Post

Jakarta Post
Rubber farmers in Mappi struggle to cope with falling
Jakarta Post
Latex has dropped to Rp 6,000 (50 US cents) per kilogram from as high as Rp 25,000 per kilogram previously.
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Lanxess launches new EdPBR rubber grades - RubberNews.com

Lanxess launches new EdPBR rubber grades - RubberNews.com | Hevea brasiliensis | Scoop.it
Lanxess launches new EdPBR rubber grades
RubberNews.com
COLOGNE, Germany—Lanzess A.G. has launched two neodymium polybutadiene rubber grades to ease the production of fuel-efficient tires.
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CoMT From Hevea brasiliensis

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A Laboratory Bioassay of the Potential Effect of Rubber Extract (Hevea brasiliensis) on the Survival of Fingerlings of Oreochromis niloticus

A Laboratory Bioassay of the Potential Effect of Rubber Extract (Hevea brasiliensis) on the Survival of Fingerlings of Oreochromis niloticus
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Abstract

The potential effects of Hevea brasiliensis on the survival of fingerlings of Oreochromis niloticus were investigated in duplicate (A and B) using the water soluble fraction of the latex under laboratory conditions for 96 hours. The WSF of Hevea brasiliensis was tested against Oreochromis niloticus at 0, 8, 16, 24, 32 and 40mg/l in glass aquaria stocked with ten animals for 96 hours under observation for changes. Behavioural pattern exhibited by the fish include, loss of balance, restlessness, attempt at jumping out and hemorrhaged gills, respiratory difficulties and mortalities were observed in the WSF exposure groups, but not in the controls. LC50 values were estimated at 28. 50 ± 0.2mg/l. There was significant difference in mortalities between the replicate group (p < 0.05), leading to conclusion that the organism in each batch responded differently to the toxic effect of WSF of Hevea brasiliensis latex.

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Optimal soil-sampling design for rubber tree management based on fuzzy clustering

Optimal soil-sampling design for rubber tree management based on fuzzy clustering | Hevea brasiliensis | Scoop.it
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Farming management practices related to nutrient recommendation for rubber tree plantations have been a challenge for scientists, farm managers and local producers. Specific caves and building contour ledges to prevent nutrient losses through soil erosion often cause spatial variation of topsoil nutrients in such plantations of rubber trees (Hevea brasiliensis). The design of soil-sampling schemes to test chemical properties of the soil is critical for successful nutrient recommendation for rubber trees. Our objectives were to characterize the spatia variability of soil pH, macronutrient NPK and organic matter in rubber plantations and to evaluate the rationality of soil sampling schemes in rubber plantations for tree nutrient management. The study was conducted in an area of 84 m2 consistent of nine rubber trees and soil samples (0–0.2 m depth) were taken from 168 grid points with a dimension of 1 m × 0.5 m. Concentrations of total nitrogen, organic matter, available phosphorus, available potassium and pH levels were determined for each soil sample. Based on their spatial variability patterns, the analyzed variables were divided into several homogeneous zones through fuzzy cluster algorithm. The number of subzones was determined using fuzzy performance index and normalized classification entropy to optimize the classification algorithm. The classification results showed that there were three optimal sampling zones for the soil chemical properties. The analysis of variance indicated that chemical properties were significantly different between the delineated zones. The delineated management zones could be used as a reference for making soil-sampling scheme in the rubber plantation. The results of this study have the implication in optimization of soil sampling planning for soil testing for nutrient recommendation. Fuzzy cluster algorithms could classify soil chemical properties into three practical zones by reducing intrazone variability, which would provide with useful information for making effective soil-sampling schemes in rubber tree plantations.

 
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LATICIFEROUS TISSUE-SPECIFIC SRPP PROMOTER FROM HEVEA BRASILIENSIS AND USES THEREOF - Korea Research Institute of Bioscience and Biotechnology

A laticiferous tissue-specific SRPP (small rubber particle-associated protein) promoter derived from Hevea brasiliensis, consists of nucleotide sequence of SEQ ID NO: 1. A recombinant plant exp
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A laticiferous tissue-specific SRPP (small rubber particle-associated protein) promoter derived from Hevea brasiliensis, consists of nucleotide sequence of SEQ ID NO: 1. A recombinant plant expression vector includes the promoter. A plant is transformed with the recombinant plant expression vector and seed of the transformed plant is obtained. A method for laticiferous tissue-specific expression of a foreign gene in a transformed plant includes performing recombination of a foreign gene into the recombinant plant expression vector. The transformed plant produced by the method shows laticiferous tissue-specific expression of a foreign gene.

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Shortwave near infrared spectroscopy for determination of dry rubber content and total solids content of Para rubber (Hevea brasiliensis) latex

Shortwave near infrared spectroscopy for determination of dry rubber content and total solids content of Para rubber (Hevea brasiliensis) latex | Hevea brasiliensis | Scoop.it
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The objective of this research was to apply short wave near infrared (NIR) (700–1100 nm) spectroscopy to the evaluation of dry rubber content (DRC, %) and total solids content (TSC, %) in Para rubber field and concentrated latex. The spectra were obtained in transmission mode. There were 282 samples including field latex, concentrated latex and concentration adjusted concentrated latex of 171, 86 and 25 samples, respectively. The field latex had around 30% DRC and the concentrated latex had around 60% DRC. The concentration adjusted concentrated latex was in five levels which were 55%, 50%, 45%, 40% and 35% DRC. The calibration models were developed by original and pretreated absorbance spectra using partial least square regression (PLSR). The pretreatment methods were smoothing, normalization, derivative, baseline offset, standard normal variate (SNV) or multiple scatter correction (MSC). By statistical analysis, the model developed from smoothing and range normalization pretreated spectra in the wavelength range of 700–950 nm provided the best accuracy of prediction for DRC and the models usingfrom raw spectra in the same wavelength range gave the best results for TSC. The slope, offset, coefficient of determination (r2), standard error of prediction (SEP) and bias were 1.0154, -0.6286, 0.992, 1.1092% and 0.0321%, respectively for the prediction of DRC and 1.0084, –0.2332, 0.991, 1.3611% and 0.1456%, respectively for the prediction of TSC. The best models were validated using new sets of 50 and 35 unknown samples for DRC and TSC, respectively. The models achieved in r2 of 0.988 and 0.974, SEP of 1.4296 and 2.1255, RDP of 9.978710.0 and 6.1728 and bias of –0.6191% and 0.5476% for DRC and TSC, respectively. This work showed the high accuracy of shortwave near infrared spectroscopy in evaluation of TSC, the essential parameter for quality assurance and process control in the concentrated rubber latex factory. However, the accuracy for prediction of DRC was still not acceptable for the latex factory because a bias of more than 0.01% is not acceptable for it would affect the amount of money which the factory may loose or gain.

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Frontiers | Impact of tapping and soil water status on fine root dynamics in a rubber tree plantation in Thailand | Functional Plant Ecology

Fine roots (FR) play a major role in the water and nutrient uptake of plants and contribute significantly to the carbon and nutrient cycles of ecosystems through their annual production and turnove...
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Characterization of a full-length cDNA clone encoding glutamine synthetase from rubber tree latex

Pujade Renaud, Valerie; Perrot Rechenmann, Catherine; Chrestin, Herve; Lacrotte, Regis; Guern, Jean, 1997: Characterization of a full-length cDNA clone encoding glutamine synthetase from rubber tree latex (Characterization of a full-length cDNA clone...
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Trunk Injection Method for Introducing Chemical Formulations in to Rubber Trees

Trunk Injection Method for Introducing Chemical Formulations in to Rubber Trees
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Production and characterization of natural rubber-Ca/P blends for biomedical purposes

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Abstract

This study presents the development of natural rubber-Ca/P blends, as promising candidates for biomedical purposes. The specific objective was the incorporation of Ca/P into a natural rubber polymeric matrix. Ca/P crystalline phases were synthesized by the sol-gel method and the polymeric matrices were produced using natural rubber extracted from latex of the Hevea brasiliensis. The shape and size of natural rubber particles present in the NR membrane, as well as, the way the Ca/P powder grains aggregate in the polymeric matrix was investigated, giving information about the interactions between the Ca/P and the natural rubber particles. Confocal fluorescence scanning microscopy measurements allowed us to propose a structure where the Ca/P grains are surrounded by natural rubber particles. This structure may mediate Ca2 + release for tissue regeneration. The system investigated may open new horizons for development of a bandage which provides the controlled-release of biomaterials.

 
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RNA sequencing read depth requirement for optimal transcriptome ...

RNA sequencing read depth requirement for optimal transcriptome ... | Hevea brasiliensis | Scoop.it
In this report, we describe the use of Illumina paired-end RNA-Seq (PE RNA-Seq) reads from Hevea… Source:RNA sequencing read depth requirement for optimal transcriptome coverage in Hevea brasiliensis ...
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Yulex, SGB collaborate on genomics platform - RubberNews.com

Yulex, SGB collaborate on genomics platform RubberNews.com SGB's work on guayule will combine breeding and germplasm selection with genomic technologies, including high-throughout genotyping, genome-wide trait association studies, genomic selection...
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Global Natural-Rubber Consumption Growth Seen Reducing Surplus - Bloomberg

Global Natural-Rubber Consumption Growth Seen Reducing Surplus - Bloomberg | Hevea brasiliensis | Scoop.it
Global Natural-Rubber Consumption Growth Seen Reducing Surplus Bloomberg World natural-rubber consumption is set to increases this year and the next, reducing a glut and boosting prices of the commodity used in tires, according to The Rubber...
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Comparative Proteomics of Primary and Secondary Lutoids Reveals that Chitinase and Glucanase Play a Crucial Combined Role in Rubber Particle Aggregation in Hevea brasiliensis

Comparative Proteomics of Primary and Secondary Lutoids Reveals that Chitinase and Glucanase Play a Crucial Combined Role in Rubber Particle Aggregation in Hevea brasiliensis | Hevea brasiliensis | Scoop.it
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Lutoids are specific vacuole-based organelles within the latex-producing laticifers in rubber tree Hevea brasiliensis. Primary and secondary lutoids are found in the primary and secondary laticifers, respectively. Although both lutoid types perform similar roles in rubber particle aggregation (RPA) and latex coagulation, they vary greatly at the morphological and proteomic levels. To compare the differential proteins and determine the shared proteins of the two lutoid types, a proteomic analysis of lutoid membranes and inclusions was performed, revealing 169 proteins that were functionally classified into 14 families. Biological function analysis revealed that most of the proteins are involved in pathogen defense, chitin catabolism, and proton transport. Comparison of the gene and protein changed patterns and determination of the specific roles of several main lutoid proteins, such as glucanase, hevamine and hevein, demonstrated that chitinase and glucanase appeared to play crucial synergistic roles in RPA. Integrative analysis revealed a protein-based metabolic network mediating pH and ion homeostasis, defense response, and RPA in lutoids. From these findings, we developed a modified regulation model for lutoid-mediated RPA that will deepen our understanding of potential mechanisms involved in lutoid-mediated RPA and consequent latex coagulation.

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Effect of Lethal Concentrations of Rubber Extract (Hevea brasiliensis) on the Survival on Fingerlings of Clarias gariepinus under Laboratory Condition.

Effect of Lethal Concentrations of Rubber Extract (Hevea brasiliensis) on the Survival on Fingerlings of Clarias gariepinus under Laboratory Condition.
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Abstract

 

The water soluble fraction (WSF) of Hevea brasiliensis was tested against Clarias garienpinus, at 0, 30, 40, 50, 60 and 70mg/l in glass aquaria stocked with ten animals for 96 hours under observation for changes. Moribund swimming, restlessness, respiratory difficulties, depigmentation and mortalities were observed in the WSF exposure groups, but not in the controls. LC50 values were estimated at 50.12mg/l. There was no significant difference in mortalities between the replicate group (P>0.05), leading to the conclusion that the WSF of Hevea brasiliensis had same toxic effects on both batches of the test organism.

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Role of ascospores and conidia in the initiation and spread of South American Leaf Blight in a rubber tree plantation -

Role of ascospores and conidia in the initiation and spread of South American Leaf Blight in a rubber tree plantation - | Hevea brasiliensis | Scoop.it
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Microcyclus ulei, the fungus causing South American Leaf Blight (SALB) on rubber tree leaves, produces two main types of spores: ascospores and conidia. To assess their respective epidemiological role, a field experiment was conducted in French Guiana over three years. Tree phenology, disease severity and climate variables were recorded, while airborne spores were trapped and quantified. Ascospores were shown to play an essential role in the perpetuation of the disease outside the host's growth periods, in the resumption of epidemics, and in the spread of the disease to disease-free zones. Conidia were trapped in visibly infected plots only, during periods of host growth. Disseminated over short distances and present only temporarily on leaves, the conidia enabled the disease to spread stepwise when the climate was conducive. Segmentation analysis revealed that the duration of high relative humidity was the climatic variable most related to ascospore trapping. Ascospore release did not require low temperatures. Considering the essential role of the ascospores in the initiation and spread of disease, artificial defoliation as a means of reducing the inoculum pressure during tree refoliation is proposed to control SALB. To check the availability of this method, a several years-survey of natural defoliation-refoliation in relation to climate and other leaf diseases is needed.

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Storage stability of fatty acid methyl esters from Hevea brasiliensis.

Biodiesel is a fast emerging alternate fuel but with its own disadvantage of having limited oxidative stability.
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Biodiesel is a fast emerging alternate fuel but with its own disadvantage of having limited oxidative stability. Fatty acid methyl esters (FAMEs) obtained from high acid value (46.41 mgKOHg-1) rubber seed oil (RSO) was characterized by GC-MS and 1H-NMR. Fuel properties analysed were found to fit the required ASTM D6751 standard. Moreover, the possibility of monitoring the conversion efficiency of RSO to its corresponding methyl esters using FT-IR and NIR techniques was also analysed. As an important specification of bio-fuel quality, the fuel must have a minimum of three hours of oxidative stability (110°C) as per ASTM D6751 standard. Influence of metal contaminants, air, moisture and light on oxidative stability was studied on biodiesel having high iodine value. Acid value, peroxide value and induction period were recorded for samples under six different environments such as open (light exposed), closed (light exposed), open (dark), closed (dark), open (metal contaminated) and closed with metal contamination. Significant impact was observed only when metals are present as contaminants. Extent of degradation (oxidation) is higher when the samples are contaminated by metals, limiting the storage stability to thirty days. Higher oxidative stability was achieved under dark in the closed environment but in the absence of any metal contaminant.

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Microsatellite Paternity Analysis Used for Evaluation of

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Construction an Characterization of BAC and Fosmid Librar of the Rubber Tre (Hevea brasiliensis Muell. Arg.)

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