Hevea brasiliensis
Follow
Find
2.8K views | +2 today
 
Scooped by Biswapriya Biswavas Misra
onto Hevea brasiliensis
Scoop.it!

JP2012050804 METHOD FOR PRODUCING ISOPRENOID, ISOPRENOID, CALLUS, METHOD FOR INDUCING CALLUS, AND METHOD FOR CULTURING CALLUS

Provided are: a method for stably producing an isoprenoid without relying on an adult tree of Hevea brasiliensis; and an isoprenoid which is obtained by the production method. Also provided are: a novel callus which is capable of producing an isoprenoid; a method for inducing the callus; and a method for culturing the callus. The present invention relates to: a method for producing an isoprenoid, wherein an isoprenoid is biosynthesized by means of a callus of an isoprenoid-producing plant; and an isoprenoid which is obtained by the production method. The present invention also relates to: a callus which is induced by culturing tissue fragments of an isoprenoid-producing plant in an induction medium that contains a plant growth hormone and a carbon source; a method for inducing a callus, wherein a callus is induced by culturing tissue fragments of an isoprenoid-producing plant in an induction medium that contains a plant growth hormone and a carbon source; and a method for culturing a callus, wherein a callus is grown by culturing a callus of an isoprenoid-producing plant in a growing medium that contains a plant growth hormone and a carbon source.

more...
No comment yet.
Your new post is loading...
Your new post is loading...
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Draft genome sequence of the rubber tree Hevea brasiliensis - 7thSpace Interactive (press release)

Draft genome sequence of the rubber tree Hevea brasiliensis 7thSpace Interactive (press release) Conclusions: The knowledge gained from this genome sequence will aid in the future development of high-yielding clones to keep up with the ever...
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Transcriptome sequencing and comparative analysis reveal long-term flowing mechanisms in Hevea brasiliensis latex.

Gene. 2014 Nov 26. pii: S0378-1119(14)01325-0. doi: 10.1016/j.gene.2014.11.048. [Epub ahead of print]
Biswapriya Biswavas Misra's insight:

The rubber tree, Hevea brasiliensis, is a major commercial source of natural rubber. Increasing the rubber yield of rubber trees is a very serious problem since the demands for high quality rubber materials are great. Establishment of a tapping system is based on an estimate of tapping intensity from the rubber tree. Latex flowing time is one of the most critical factors that determine the rubber yield. Long-term flow is a type of phenomenon of the rubber tree latex with longer flowing time than normal latex flow, and is always caused by intensive tapping. Thus, transcriptome and expression profiling data for long-term flowing latex (LFL) are needed as an important resource to identify genes and to better understand the biological mechanisms of latex flow in rubber trees.

RESULTS:

The transcripts were sequenced using the Illumina sequencing platform. After cleaning, quality checks and sequencing, 98,697 transcripts and 38,584 unigenes were assembled with the mean size of 1437.31bp and 923.86bp, respectively. In BLAST searches of our database against public databases, 65.17% (25,147) of the unigenes were annotated with gene descriptions, conserved protein domains, or gene ontology terms. Functional categorization further revealed 853 individual unigenes related to long-term flow. According to KEGG classification, the clusters for "cysteine and methionine metabolism", "energy", "oxidative phosphorylation", "terpenoid backbone biosynthesis", "plant hormone signal transduction" and "copper, potassium transporter" were significantly enriched metabolic pathways.

CONCLUSIONS:

We conducted high-resolution transcriptome profiling related to LFL in H. brasiliensis. The research facilitates further studies on gene discovery and on the molecular mechanisms related to the estimation of tapping intensity and prolonging latex flowing time. We concluded that it was necessary to improve energy supplies for intensive tapping and the copper ion content of rubber tree latex could be considered as a standard to estimate tapping intensity.

more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Cloning and characterisation of JAZ gene family in Hevea brasiliensis.

Mechanical wounding or treatment with exogenous jasmonates (JA) induces differentiation of the laticifer in Hevea brasiliensis. JA is a key signal for latex biosynthesis and wounding response in the rubber tree. Identification of JAZ (jasmonate ZIM-domain) family of proteins that repress JA responses has facilitated rapid progress in understanding how this lipid-derived hormone controls gene expression and related physiological processes in plants. In this work, the full-length cDNAs of six JAZ genes were cloned from H. brasiliensis (termed HbJAZ). These HbJAZ have different lengths and sequence diversity, but all of them contain Jas and ZIM domains, and two of them contain an ERF-associated amphiphilic repression (EAR) motif in the N-terminal. Real-time RT-PCR analyses revealed that HbJAZ have different expression patterns and tissue specificity. Four HbJAZ were up-regulated, one was down-regulated, while two were less effected by rubber tapping treatment, suggesting that they might play distinct roles in the wounding response. A yeast two-hybrid assay revealed that HbJAZ proteins interact with each other to form homologous or heterogeneous dimer complexes, indicating that the HbJAZ proteins may expand their function through diverse JAZ-JAZ interactions. This work lays a foundation for identification of the JA signalling pathway and molecular mechanisms of latex biosynthesis in rubber trees. This article is protected by copyright. All rights reserved.
Biswapriya Biswavas Misra's insight:

Mechanical wounding or treatment with exogenous jasmonates (JA) induces differentiation of the laticifer in Hevea brasiliensis. JA is a key signal for latex biosynthesis and wounding response in the rubber tree. Identification of JAZ (jasmonate ZIM-domain) family of proteins that repress JA responses has facilitated rapid progress in understanding how this lipid-derived hormone controls gene expression and related physiological processes in plants. In this work, the full-length cDNAs of six JAZ genes were cloned from H. brasiliensis (termed HbJAZ). These HbJAZ have different lengths and sequence diversity, but all of them contain Jas and ZIM domains, and two of them contain an ERF-associated amphiphilic repression (EAR) motif in the N-terminal. Real-time RT-PCR analyses revealed that HbJAZ have different expression patterns and tissue specificity. Four HbJAZ were up-regulated, one was down-regulated, while two were less effected by rubber tapping treatment, suggesting that they might play distinct roles in the wounding response. A yeast two-hybrid assay revealed that HbJAZ proteins interact with each other to form homologous or heterogeneous dimer complexes, indicating that the HbJAZ proteins may expand their function through diverse JAZ-JAZ interactions. This work lays a foundation for identification of the JA signalling pathway and molecular mechanisms of latex biosynthesis in rubber trees. This article is protected by copyright. All rights reserved.

more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

De Novo Assembly and Transcriptome Analysis of the Rubber Tree ...

De Novo Assembly and Transcriptome Analysis of the Rubber Tree ... | Hevea brasiliensis | Scoop.it
Hevea brasiliensis (Willd. Ex Adr. Juss.) Muell.-Arg. is the primary source of natural rubber that is native to the Amazon rainforest. The singular properties of natural rubber make it superior to and competitive with synthetic ...
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Prevention of biodeterioration of rubberwood Hevea brasiliensis using K-Otek

Ananthapadmanabha, H. S.; Srinivasan, V.
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Frontiers | Impact of tapping and soil water status on fine root dynamics in a rubber tree plantation in Thailand | Functional Plant Ecology

Fine roots (FR) play a major role in the water and nutrient uptake of plants and contribute significantly to the carbon and nutrient cycles of ecosystems through their annual production and turnove...
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Characterization of a full-length cDNA clone encoding glutamine synthetase from rubber tree latex

Pujade Renaud, Valerie; Perrot Rechenmann, Catherine; Chrestin, Herve; Lacrotte, Regis; Guern, Jean, 1997: Characterization of a full-length cDNA clone encoding glutamine synthetase from rubber tree latex (Characterization of a full-length cDNA clone...
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Trunk Injection Method for Introducing Chemical Formulations in to Rubber Trees

Trunk Injection Method for Introducing Chemical Formulations in to Rubber Trees
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Production and characterization of natural rubber-Ca/P blends for biomedical purposes

Biswapriya Biswavas Misra's insight:
Abstract

This study presents the development of natural rubber-Ca/P blends, as promising candidates for biomedical purposes. The specific objective was the incorporation of Ca/P into a natural rubber polymeric matrix. Ca/P crystalline phases were synthesized by the sol-gel method and the polymeric matrices were produced using natural rubber extracted from latex of the Hevea brasiliensis. The shape and size of natural rubber particles present in the NR membrane, as well as, the way the Ca/P powder grains aggregate in the polymeric matrix was investigated, giving information about the interactions between the Ca/P and the natural rubber particles. Confocal fluorescence scanning microscopy measurements allowed us to propose a structure where the Ca/P grains are surrounded by natural rubber particles. This structure may mediate Ca2 + release for tissue regeneration. The system investigated may open new horizons for development of a bandage which provides the controlled-release of biomaterials.

 
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

RNA sequencing read depth requirement for optimal transcriptome ...

RNA sequencing read depth requirement for optimal transcriptome ... | Hevea brasiliensis | Scoop.it
In this report, we describe the use of Illumina paired-end RNA-Seq (PE RNA-Seq) reads from Hevea… Source:RNA sequencing read depth requirement for optimal transcriptome coverage in Hevea brasiliensis ...
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Yulex, SGB collaborate on genomics platform - RubberNews.com

Yulex, SGB collaborate on genomics platform RubberNews.com SGB's work on guayule will combine breeding and germplasm selection with genomic technologies, including high-throughout genotyping, genome-wide trait association studies, genomic selection...
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Global Natural-Rubber Consumption Growth Seen Reducing Surplus - Bloomberg

Global Natural-Rubber Consumption Growth Seen Reducing Surplus - Bloomberg | Hevea brasiliensis | Scoop.it
Global Natural-Rubber Consumption Growth Seen Reducing Surplus Bloomberg World natural-rubber consumption is set to increases this year and the next, reducing a glut and boosting prices of the commodity used in tires, according to The Rubber...
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Comparative Proteomics of Primary and Secondary Lutoids Reveals that Chitinase and Glucanase Play a Crucial Combined Role in Rubber Particle Aggregation in Hevea brasiliensis

Comparative Proteomics of Primary and Secondary Lutoids Reveals that Chitinase and Glucanase Play a Crucial Combined Role in Rubber Particle Aggregation in Hevea brasiliensis | Hevea brasiliensis | Scoop.it
Biswapriya Biswavas Misra's insight:

Lutoids are specific vacuole-based organelles within the latex-producing laticifers in rubber tree Hevea brasiliensis. Primary and secondary lutoids are found in the primary and secondary laticifers, respectively. Although both lutoid types perform similar roles in rubber particle aggregation (RPA) and latex coagulation, they vary greatly at the morphological and proteomic levels. To compare the differential proteins and determine the shared proteins of the two lutoid types, a proteomic analysis of lutoid membranes and inclusions was performed, revealing 169 proteins that were functionally classified into 14 families. Biological function analysis revealed that most of the proteins are involved in pathogen defense, chitin catabolism, and proton transport. Comparison of the gene and protein changed patterns and determination of the specific roles of several main lutoid proteins, such as glucanase, hevamine and hevein, demonstrated that chitinase and glucanase appeared to play crucial synergistic roles in RPA. Integrative analysis revealed a protein-based metabolic network mediating pH and ion homeostasis, defense response, and RPA in lutoids. From these findings, we developed a modified regulation model for lutoid-mediated RPA that will deepen our understanding of potential mechanisms involved in lutoid-mediated RPA and consequent latex coagulation.

more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Molecular cloning and characterization of a Mlo gene in rubber tree (Hevea brasiliensis).

Mlo gene encodes a plant-specific seven-transmembrane domain protein involved in a variety of cellular processes. In this study, a novel Mlo gene from rubber tree (Hevea brasiliensis), designated HbMlo1, was cloned by RT-PCR in rubber tree. The ORF of HbMlo1 was 1551bp in length, encoding a putative protein of 516 amino acids. HbMlo1 was a typical Mlo protein with seven-transmembrane domain. Sequence comparison between HbMlo1 and other Mlo proteins demonstrated that HbMlo1 shared the highest similarity with the Cucumis melo CmMlo1 and Arabidopsis thaliana AtMlo1 with 75.1% and 71.3% sequence identity, respectively. Phylogenetic analysis revealed that HbMlo1, CmMlo1, AtMlo1, AtMlo13, and AtMlo15 formed into the phylogenetic clade II with 100% bootstrap support value. HbMlo1 transcript exhibited tissue specificity, and it was preferentially expressed in leaf. Furthermore, the amount of HbMlo1 transcript was significantly induced by various phytohormones (including ethephon, methyl jasmonate, salicylic acid, abscisic acid, indole-3-acetic acid, and gibberellic acid), H2O2, and wounding treatments. Under drought stress, HbMlo1 exhibited a complex pattern of regulation. However, HbMlo1 expression did not significantly change during powdery mildew infection. These results suggested that HbMlo1 might play a role in phytohormone signaling and abiotic stress response processes in rubber tree.
Biswapriya Biswavas Misra's insight:

Mlo gene encodes a plant-specific seven-transmembrane domain protein involved in a variety of cellular processes. In this study, a novel Mlo gene from rubber tree (Hevea brasiliensis), designated HbMlo1, was cloned by RT-PCR in rubber tree. The ORF of HbMlo1 was 1551bp in length, encoding a putative protein of 516 amino acids. HbMlo1 was a typical Mlo protein with seven-transmembrane domain. Sequence comparison between HbMlo1 and other Mlo proteins demonstrated that HbMlo1 shared the highest similarity with the Cucumis melo CmMlo1 and Arabidopsis thaliana AtMlo1 with 75.1% and 71.3% sequence identity, respectively. Phylogenetic analysis revealed that HbMlo1, CmMlo1, AtMlo1, AtMlo13, and AtMlo15 formed into the phylogenetic clade II with 100% bootstrap support value. HbMlo1 transcript exhibited tissue specificity, and it was preferentially expressed in leaf. Furthermore, the amount of HbMlo1 transcript was significantly induced by various phytohormones (including ethephon, methyl jasmonate, salicylic acid, abscisic acid, indole-3-acetic acid, and gibberellic acid), H2O2, and wounding treatments. Under drought stress, HbMlo1 exhibited a complex pattern of regulation. However, HbMlo1 expression did not significantly change during powdery mildew infection. These results suggested that HbMlo1 might play a role in phytohormone signaling and abiotic stress response processes in rubber tree.

more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Genetic and epigenetic uniformity of polyembryony derived multiple seedlings of Hevea brasiliensis.

Genetic and epigenetic uniformity of polyembryony derived multiple seedlings of Hevea brasiliensis. | Hevea brasiliensis | Scoop.it
Hevea brasiliensis Muell. Arg (Para rubber tree) is a tropical tree species of Amazonian origin widely cultivated in several parts of the world for natural rubber, a highly priced commodity inevitable for the world rubber industry. Large, tree to tree variation in growth and latex yield among individual plants of high yielding Hevea clones is a common phenomenon observed in mature rubber plantations. The genetic heterogeneity of the seedlings which are used as rootstocks for propagation through budgrafting is considered as a major factor responsible for this variation. In order to minimize this variation, attempts were made to develop highly uniform rootstock material via an in vitro technique by inducing zygotic polyembryony in Hevea. Immature open pollinated fruits of a high yielding clone RRII 105 were cultured by half ovulo embryo culture technique. Multiple embryos were induced from the 8-10-week-old zygote with a novel combination of gibberellic acid (GA3), kinetin, and zeatin. Plantlets were successfully generated from the multiple embryos and raised in the field post hardening. Screening using genetic and epigenetic molecular markers revealed that the multiple seedlings developed are highly uniform and are of single zygotic origin. Development of plants having genetic and epigenetic uniformity suggests that this technique is ideal for raising uniform rootstock material in Hevea which may significantly reduce intraclonal variations. Moreover, these plants could serve as ideal material for physiological and molecular investigations towards the understanding of stock-scion interaction process in rubber.
Biswapriya Biswavas Misra's insight:

Hevea brasiliensis Muell. Arg (Para rubber tree) is a tropical tree species of Amazonian origin widely cultivated in several parts of the world for natural rubber, a highly priced commodity inevitable for the world rubber industry. Large, tree to tree variation in growth and latex yield among individual plants of high yielding Hevea clones is a common phenomenon observed in mature rubber plantations. The genetic heterogeneity of the seedlings which are used as rootstocks for propagation through budgrafting is considered as a major factor  responsible for this variation. In order to minimize this variation, attempts were made to develop highly uniform rootstock material via an in vitro technique by inducing zygotic polyembryony in Hevea. Immature open pollinated fruits of a high yielding clone RRII 105 were cultured by half ovulo embryo culture technique. Multiple embryos were induced from the 8-10-week-old zygote with a novel combination of gibberellic acid (GA3), kinetin, and zeatin. Plantlets were successfully generated from the multiple embryos and raised in the field post hardening. Screening using genetic and epigenetic molecular markers revealed that the multiple seedlings developed are highly uniform and are of single zygotic origin. Development of plants having genetic and epigenetic uniformity suggests that this technique is ideal for raising uniform rootstock material in Hevea which may significantly reduce intraclonal variations. Moreover, these plants could serve as ideal material for physiological and molecular investigations towards the understanding of  stock-scion interaction process in rubber.

more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain - Martínez-Caballero - FEBS Journal - Wiley Online Library

Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain - Martínez-Caballero - FEBS Journal - Wiley Online Library | Hevea brasiliensis | Scoop.it
Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate... http://t.co/qdz8NqgFNI
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Molecular cloning and expression of a novel MYB... [Mol Biol Rep. 2014] - PubMed - NCBI

PubMed comprises more than 23 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Responses of rubber tree clone RRIM 600 to NPK fertilization on red yellow podzolic soil

Bataglia, Ondino Cleante; Dos Santos, Wagner Rodrigues; Igue, Toshio; De Souza Goncalves, Paulo, 1998: Responses of rubber tree clone RRIM 600 to NPK fertilization on red yellow podzolic soil (Responses of rubber tree clone RRIM 600 to NPK fertilization...
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

De novo transcriptome analysis of Hevea brasiliensis tissues by RNA-seq and screening for molecular markers

The rubber tree, Hevea brasiliensis, is a species native to the Brazilian Amazon region and it supplies almost all the world's natural rubber, a strategic raw material for a variety of products. One of the major challenges for developing rubber tree plantations is adapting the plant to biotic and abiotic stress. Transcriptome analysis is one of the main approaches for identifying the complete set of active genes in a cell or tissue for a specific developmental stage or physiological condition.
Biswapriya Biswavas Misra's insight:
Background

The rubber tree, Hevea brasiliensis, is a species native to the Brazilian Amazon region and it supplies almost all the world's natural rubber, a strategic raw material for a variety of products. One of the major challenges for developing rubber tree plantations is adapting the plant to biotic and abiotic stress. Transcriptome analysis is one of the main approaches for identifying the complete set of active genes in a cell or tissue for a specific developmental stage or physiological condition.

Results

Here, we report on the sequencing, assembling, annotation and screening for molecular markers from a pool of H. brasiliensis tissues. A total of 17,166 contigs were successfully annotated. Then, 2,191 Single Nucleotide Variation (SNV) and 1.397 Simple Sequence Repeat (SSR) loci were discriminated from the sequences. From 306 putative, mainly non-synonymous SNVs located in CDS sequences, 191 were checked for their ability to characterize 23 Hevea genotypes by an allele-specific amplification technology. For 172 (90%), the nucleotide variation at the predicted genomic location was confirmed, thus validating the different steps from sequencing to the in silico detection of the SNVs.

Conclusions

This is the first study of the H. brasiliensis transcriptome, covering a wide range of tissues and organs, leading to the production of the first developed SNP markers. This process could be amplified to a larger set of in silico detected SNVs in expressed genes in order to increase the marker density in available and future genetic maps. The results obtained in this study will contribute to the H. brasiliensis genetic breeding program focused on improving of disease resistance and latex yield.

more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Phosphate Solubilizing Bacteria and Fungi Isolated from Rubber (Hevea brasiliensis) Root Rhizosphere, Their Biofilm Formation and Phosphate Solubilizing Abilities | Hettiarachchi

Phosphate Solubilizing Bacteria and Fungi Isolated from Rubber (Hevea brasiliensis) Root Rhizosphere, Their Biofilm Formation and Phosphate Solubilizing Abilities
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript

Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript - up-to-the-minute news and headlines.
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

(IUCr) Structural analysis of the endogenous glycoallergen Hev b 2 ...

Endogenous glycosylated Hev b 2 (endo- [beta] -1,3-glucanase) from Hevea brasiliensis is an important latex allergen that is recognized by IgE antibodies from patients who suffer from latex allergy. The carbohydrate moieties of Hev b 2 ...
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Rubber farmers in Mappi struggle to cope with falling - Jakarta Post

Jakarta Post
Rubber farmers in Mappi struggle to cope with falling
Jakarta Post
Latex has dropped to Rp 6,000 (50 US cents) per kilogram from as high as Rp 25,000 per kilogram previously.
more...
No comment yet.
Scooped by Biswapriya Biswavas Misra
Scoop.it!

Lanxess launches new EdPBR rubber grades - RubberNews.com

Lanxess launches new EdPBR rubber grades - RubberNews.com | Hevea brasiliensis | Scoop.it
Lanxess launches new EdPBR rubber grades
RubberNews.com
COLOGNE, Germany—Lanzess A.G. has launched two neodymium polybutadiene rubber grades to ease the production of fuel-efficient tires.
more...
No comment yet.