Of interest to the Ralstonia solanacearum community:
Two novel noncoding RNAs (ncRNAs) are predicted in the chromosome of the R. solanacearum GMI1000. These ncRNAs are homologous to those identified by this study in Burkholderia cenocepacia J2315. In GMI1000 genome the first predicted ncRNA is in the intergenic region between add (RSc2098) and guaD (RSc2099). While the second prediction lays downstream argB (RSc0033) and upstream RSc0034, a RNA polymerase sigma factor. In Burkholderia this second ncRNA seems to be upstream a transmembrane sensor histidine kinase transcription regulator protein (which is also present in R. solanacearum, downstream the RNA-pol sigma factor - RSc0039).
Although the aim of the authors (from Michigan State University) was not to produce a list of ncRNA and pseudo-ncRNAs in the genomes of these two organism, they succeded settling new ground for the re-analysis of genomic and transcriptomic data. A hard task that only bioinformaticians are able to take and enjoy =) . Discovery of new ncRNAs have been hindered by the use of conventional sequence comparison algorithms, which rely on high sequence concervation. The method developed is said to be more sensitive in identifying ncRNAs with low sequence conservation.
Looking forward future results and experimental validation of these two ncRNA candidates.