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Rescooped by Alejandra Valadez from Natural Products Chemistry Breaking News
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Inducing Secondary Metabolite Production by the Endophytic Fungus Fusarium tricinctum through Coculture with Bacillus subtilis

Inducing Secondary Metabolite Production by the Endophytic Fungus Fusarium tricinctum through Coculture with Bacillus subtilis | Education | Scoop.it

Coculturing the fungal endophyte Fusarium tricinctum with the bacterium Bacillus subtilis 168 trpC2 on solid rice medium resulted in an up to 78-fold increase in the accumulation in constitutively present secondary metabolites that included lateropyrone (5), cyclic depsipeptides of the enniatin type (6–8), and the lipopeptide fusaristatin A (9). In addition, four compounds (1–4) including (−)-citreoisocoumarin (2) as well as three new natural products (1, 3, and 4) were not present in discrete fungal and bacterial controls and only detected in the cocultures. The new compounds were identified as macrocarpon C (1), 2-(carboxymethylamino)benzoic acid (3), and (−)-citreoisocoumarinol (4) by analysis of the 1D and 2D NMR and HRMS data. Enniatins B1 (7) and A1 (8), whose production was particularly enhanced, inhibited the growth of the cocultivated B. subtilis strain with minimal inhibitory concentrations (MICs) of 16 and 8 μg/mL, respectively, and were also active against Staphylococcus aureus, Streptococcus pneumoniae, and Enterococcus faecalis with MIC values in the range 2–8 μg/mL. In addition, lateropyrone (5), which was constitutively present in F. tricinctum, displayed good antibacterial activity against B. subtilis, S. aureus, S. pneumoniae, and E. faecalis, with MIC values ranging from 2 to 8 μg/mL. All active compounds were equally effective against a multiresistant clinical isolate of S. aureus and a susceptible reference strain of the same species.

 

 

Antonius R. B. Ola†‡, Dhana Thomy†, Daowan Lai*†, Heike Brötz-Oesterhelt†, and Peter ProkschJ. Nat. Prod., Article ASAPDOI: 10.1021/np400589h

 


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Rescooped by Alejandra Valadez from TIC educación
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Educación | Organización de las Naciones Unidas para la Educación, la Ciencia y la Cultura

Educación | Organización de las Naciones Unidas para la Educación, la Ciencia y la Cultura | Education | Scoop.it
La UNESCO considera que la educación es un factor esencial para el desarrollo humano, social y económico, y fomenta un mundo sostenible en el que se aprecie el conocimiento, promueve la cultura de paz, ensalza la diversidad y defiende los derechos...

Via claudia ines castiglioni
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Alejandra Ximena Pauca Peralta's comment, April 13, 2013 5:32 PM
Necesito obtener toda la informacion porfavor !