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DNA & RNA Research
The latest news and information about DNA- and RNA-related research
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Knockdown of lncRNAs: exploring RNAi and antisense oligo methods

Thousands of different long non-coding RNAs (lncRNAs) exist in mammalian cells. lncRNAs do not encode proteins but can be very important for cell function. Studying their functions can be difficult because of their diverse modes of action. One method to discern cellular function is by selective knockdown of a specific lncRNA species. However, achieving consistent knockdown has proven to be more challenging for lncRNAs than for mRNAs or miRNAs. In this presentation, we discuss some of the issues encountered with lncRNA research. We cover antisense oligonucleotide (ASO) and small interfering RNA (siRNA) methods for lncRNA knockdown. And, we show how cellular localization of a specific lncRNA target informs the choice of knockdown method.

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Making Nanowires from Protein and DNA

Making Nanowires from Protein and DNA | DNA & RNA Research | Scoop.it

Using computational and experimental methods, researchers at Caltech have developed a technique for creating so-called protein–DNA nanowires—a hybrid biomaterial that could have important applications.

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Hacking the body: the scientific counter-culture of the DIYbio movement

Hacking the body: the scientific counter-culture of the DIYbio movement | DNA & RNA Research | Scoop.it

There's a new counter-culture movement that is seeking to bypass the bureaucracy of science and hack biology for the benefit of the masses.

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CRISPR here, CRISPR there, CRISPR CRISPR everywhere

CRISPR here, CRISPR there, CRISPR CRISPR everywhere | DNA & RNA Research | Scoop.it

Scientists have long dreamed of the ability to make targeted genomic changes: precise and specific alterations in an organism’s DNA to affect its phenotype. Recently, this dream has become a reality through the discovery and engineering of nucleases that can be targeted to precise genomic locations.


Via Mary Williams
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Replacing Pesticides With Genetics

Replacing Pesticides With Genetics | DNA & RNA Research | Scoop.it

Scientists have genetically modified thousands of diamondback moths, infusing the farm pests with DNA designed to kill female larvae.

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Design of "Japonica Array" - a SNP Array Optimized for the Japanese Population

Design of "Japonica Array" - a SNP Array Optimized for the Japanese Population | DNA & RNA Research | Scoop.it

Researchers have successfully designed the first ever SNP*1 array optimized for Japanese people.

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Genetic data reveals how wild boar became farmyard pigs

Genetic data reveals how wild boar became farmyard pigs | DNA & RNA Research | Scoop.it

Ever thought how the ingredients for that bacon sandwich got to your plate? By that, I mean the amazing historical journey that has transformed the animal and plant species we farm today into the huge global biomass that now feeds billions of us.

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Giant study poses DNA data-sharing dilemma

Giant study poses DNA data-sharing dilemma | DNA & RNA Research | Scoop.it

US Precision Medicine Initiative must decide how much data to release to participants.

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DNA-Guided 3-D Printing of Human Tissue is Unveiled

DNA-Guided 3-D Printing of Human Tissue is Unveiled | DNA & RNA Research | Scoop.it

A UCSF-led team has developed a technique to build tiny models of human tissues, called organoids, more precisely than ever before using a process that turns human cells into a biological equivalent of LEGO bricks. These mini-tissues in a dish can be used to study how particular structural features of tissue affect normal growth or go awry in cancer. They could be used for therapeutic drug screening and to help teach researchers how to grow whole human organs.

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A Programming Language for DNA Computing

A Programming Language for DNA Computing | DNA & RNA Research | Scoop.it

There has been substantial work on using DNA strands to perform specific computational tasks. This project explores how to define a low-level DNA machine language, which can be used as a target for compiling higher-level programs. The reactions of the language correspond to interactions between physical DNA strands, while the kinetics of the language correspond to the underlying kinetics of DNA.

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The DNA damage response goes viral: a way in for new cancer treatments

The DNA damage response goes viral: a way in for new cancer treatments | DNA & RNA Research | Scoop.it
Salk researchers show how DNA repair proteins sound the alarm to threats, pointing to a novel cancer therapy
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Before nature selects, gene networks steer a course for evolution

Before nature selects, gene networks steer a course for evolution | DNA & RNA Research | Scoop.it

Natural selection is a race to reproduce, a competition between individuals with varying traits that helps direct the evolution of a species. As scientists begin to explore the complex networks of genes that shape the form and function of each individual, they can ask a new question about evolution: How do the structures of these gene networks determine which individuals appear on the starting line, silently influencing evolution before competition has even begun?

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Variations in cell programs control cancer and normal stem cells

Variations in cell programs control cancer and normal stem cells | DNA & RNA Research | Scoop.it

In the breast, cancer stem cells and normal stem cells can arise from different cell types but tap into distinct yet related stem cell programs, according to Whitehead Institute researchers. The differences between these stem cell programs may be significant enough to be exploited by future therapeutics.

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Come here and be quiet! Genes physically held in silencing 'lock-down' in embryonic stem cells

Come here and be quiet! Genes physically held in silencing 'lock-down' in embryonic stem cells | DNA & RNA Research | Scoop.it

Researchers at the Babraham Institute have discovered a strong physical gene interaction network that is responsible for holding genes in a silencing grip during early development.

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La Jolla library opens biotech lab

La Jolla library opens biotech lab | DNA & RNA Research | Scoop.it

A biotech lab has opened in a La Jolla public library, possibly the first such lab in the nation. It highlights the emphasis on San Diego's large life science community.

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We must keep using CRISPR-Cas9 technology

We must keep using CRISPR-Cas9 technology | DNA & RNA Research | Scoop.it

Being able to edit the human genome is not a new idea or capability. For decades researchers have been developing potential gene therapy techniques to correct missing or faulty DNA and restore healthy gene expression in cells.

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Are Statins Bad For Me? Personalized Medicine Can't Yet Say

Are Statins Bad For Me? Personalized Medicine Can't Yet Say | DNA & RNA Research | Scoop.it

Statins made her feel wretched, so she took a DNA test to find out why. But even the doctor with the genetic testing company admits that the test doesn't tell you much more than you already know.

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Time-Lapse Analysis Offers New Look at How Cells Repair DNA Damage

Time-Lapse Analysis Offers New Look at How Cells Repair DNA Damage | DNA & RNA Research | Scoop.it

Time-lapse imaging can make complicated processes easier to grasp—think of a stitched-together sequence of photos that chronicles the construction of a building. Now, scientists from the Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab) are using a similar approach to study how cells repair DNA damage.

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Molecular cut-and-paste method improves on pasting

Molecular cut-and-paste method improves on pasting | DNA & RNA Research | Scoop.it

An article in Genome Medicine reports on new means to increase efficiency of homology-directed DNA repair in CRISPR/Cas9 genome editing experiments.

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Alexis Holwerda's curator insight, September 2, 8:42 AM

Not actually for GIS, but CRISPR is supa cool

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Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve

Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve | DNA & RNA Research | Scoop.it

Comprehensive biotic surveys, or ‘all taxon biodiversity inventories’ (ATBI), have traditionally been limited in scale or scope due to the complications surrounding specimen sorting and species identification. To circumvent these issues, several ATBI projects have successfully integrated DNA barcoding into their identification procedures and witnessed acceleration in their surveys and subsequent increase in project scope and scale. The Biodiversity Institute of Ontario partnered with the rare Charitable Research Reserve and delegates of the 6th International Barcode of Life Conference to complete its own rapid, barcode-assisted ATBI of an established land trust in Cambridge, Ontario, Canada.

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The structure of DNA by direct imaging

The structure of DNA by direct imaging | DNA & RNA Research | Scoop.it

The structure of DNA was determined in 1953 by x-ray fiber diffraction. Several attempts have been made to obtain a direct image of DNA with alternative techniques. The direct image is intended to allow a quantitative evaluation of all relevant characteristic lengths present in a molecule. A direct image of DNA, which is different from diffraction in the reciprocal space, is difficult to obtain for two main reasons: the intrinsic very low contrast of the elements that form the molecule and the difficulty of preparing the sample while preserving its pristine shape and size. We show that through a preparation procedure compatible with the DNA physiological conditions, a direct image of a single suspended DNA molecule can be obtained. In the image, all relevant lengths of A-form DNA are measurable. A high-resolution transmission electron microscope that operates at 80 keV with an ultimate resolution of 1.5 Å was used for this experiment. Direct imaging of a single molecule can be used as a method to address biological problems that require knowledge at the single-molecule level, given that the average information obtained by x-ray diffraction of crystals or fibers is not sufficient for detailed structure determination, or when crystals cannot be obtained from biological molecules or are not sufficient in understanding multiple protein configurations.

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Intact, single cell analysis of protein and nucleic acid biomarkers for clinical diagnostics

Intact, single cell analysis of protein and nucleic acid biomarkers for clinical diagnostics | DNA & RNA Research | Scoop.it

One of IDT’s GMP customers, IncellDx is a unique molecular diagnostics company with high throughput cellular analysis technologies and assays that can simultaneously identify specific protein, RNA, and DNA species within intact cells. They describe their technology and the product quality and services the IDT GMP division provides.

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Researchers Use DNA ‘Clews’ to Shuttle CRISPR-Cas9 Gene-Editing Tool into Cells

Researchers Use DNA ‘Clews’ to Shuttle CRISPR-Cas9 Gene-Editing Tool into Cells | DNA & RNA Research | Scoop.it

Researchers from North Carolina State University and the University of North Carolina at Chapel Hill have for the first time created and used a nanoscale vehicle made of DNA to deliver a CRISPR-Cas9 gene-editing tool into cells in both cell culture and an animal model.

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Zhang lab unlocks crystal structure of new CRISPR/Cas9 genome editing tool

Zhang lab unlocks crystal structure of new CRISPR/Cas9 genome editing tool | DNA & RNA Research | Scoop.it

In a paper published today in Cell researchers from the Broad Institute and University of Tokyo revealed the crystal structure of theStaphylococcus aureus Cas9 complex (SaCas9)—a highly efficient enzyme that overcomes one of the primary challenges to in vivo mammalian genome editing.

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