Next-generation sequencing (NGS)
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Next-generation sequencing (NGS)
NGS is a massively parallel DNA sequencing technology. Serving as the cornerstone of modern genomics, it empowers scientists to answer increasingly complex and impactful biological questions. http://www.idtdna.com/xgen
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Unique, dual-matched adapters mitigate index hopping between NGS samples

The increasing throughput of NGS platforms has fueled the demand to sequence many samples in parallel, also referred to as multiplex sequencing. During multiplex sequencing, the identity of each sample library within a pool is maintained using index sequences that are subsequently separated in a process called demultiplexing during data analysis. Historically, a relatively small number of unique sequences (8 x i5 and 12 x i7) were used to create index combinations to multiplex samples. Unfortunately, with this combinatorial approach, a single index swap may cause a read to be mis-assigned to a different sample causing cross-talk. In this presentation, we discuss some sources of sample cross-talk, including index hopping during cluster amplification or multiplexed capture, and how index sequencing errors may lead to demultiplexing mistakes. We discuss how sample cross-talk causes demultiplexing errors and present a method for increasing the accuracy of sample identification using unique, dual-matched index adapters.

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Genetic sequencing unravels rare disease mysteries

Genetic sequencing unravels rare disease mysteries | Next-generation sequencing (NGS) | Scoop.it

UCLA was among the first medical centers to use exome sequencing, which can analyze more than 20,000 genes at once.

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Germans provide big boost for sequencing often ignored organisms

Germans provide big boost for sequencing often ignored organisms | Next-generation sequencing (NGS) | Scoop.it

One of Germany’s states has provided a big boost for biologists who want to decipher the genomes of organisms that don’t get much attention. This week, the state of Hessen, which includes Frankfurt, awarded its local institutions €17.6 million, the first half of a 7-year grant for sequencing plants, animals, and fungi. The award includes funding for the high-quality sequencing of about 700 organisms, and for the partial sequencing or resequencing of thousands more.

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Team Modifies Molecular Inversion Probes to Capture Kilobase-Sized DNA for Cloning, Sequencing

Team Modifies Molecular Inversion Probes to Capture Kilobase-Sized DNA for Cloning, Sequencing | Next-generation sequencing (NGS) | Scoop.it

The approach, published earlier this week in Nature Biomedical Engineering, uses long-adapter single-strand oligonucleotide (LASSO) probes.

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One in five 'healthy' adults may carry disease-related genetic mutations

One in five 'healthy' adults may carry disease-related genetic mutations | Next-generation sequencing (NGS) | Scoop.it

Two new studies feed the debate over whether genome sequencing should be key part of primary care

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Researchers use long-read genome sequencing for first time in a patient

Researchers use long-read genome sequencing for first time in a patient | Next-generation sequencing (NGS) | Scoop.it

Stanford scientists have used a next-generation technology called long-read sequencing to diagnose a patient’s rare genetic condition that current technology failed to diagnose.

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The Gadget that Makes DNA Sequencing Child’s Play

The Gadget that Makes DNA Sequencing Child’s Play | Next-generation sequencing (NGS) | Scoop.it

The MinION cracks open biotech to the masses the way the PC democratized computing. What will we do with this newfound power?

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Newly-published spinach genome will make more than Popeye stronger 

Newly-published spinach genome will make more than Popeye stronger  | Next-generation sequencing (NGS) | Scoop.it

Today in Nature Communications, researchers from BTI and the Shanghai Normal University report a new draft genome of Spinacia oleracea, better known as spinach. Additionally, the authors have sequenced the transcriptomes (all the RNA) of 120 cultivated and wild spinach plants, which has allowed them to identify which genetic changes have occurred due to domestication.

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RNA sequencing applied as a tool to solve patients’ diagnostic mysteries 

RNA sequencing applied as a tool to solve patients’ diagnostic mysteries  | Next-generation sequencing (NGS) | Scoop.it

Broad Institute scientists deploy RNA sequencing to nail down disease-causing gene mutations in patients for whom genetic analysis failed to return a diagnosis. The study demonstrates the power of RNA sequencing to augment standard diagnostic tools in the clinic.

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Analyzing the exome—focusing your NGS analysis with high performance target capture

As next generation sequencing has moved into the clinic, there is an increased demand for accuracy and reproducibility. Target enrichment is needed for applications where high read depth is critical, but some performance limitations, especially in GC-rich regions of the genome, have raised questions about the overall usefulness of target capture methods. In this presentation, Dr Kristina Giorda presents a method using individually synthesized and quality checked capture baits that performs well, even for GC-rich sequences, and delivers accurate coverage of the target space. Dr Giorda covers library preparation and target capture, and shares informative data generated using our xGen® Exome Research Panel.

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The Earth BioGenome Project Aims to Sequence All Eukaryotic Species

The Earth BioGenome Project Aims to Sequence All Eukaryotic Species | Next-generation sequencing (NGS) | Scoop.it

An unfunded project with an ambitious goal promises to shed light on the evolutionary history of higher organisms and enhance conservation efforts. But is the $4.8 billion project well-conceived?

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Genes key to killer bee’s success

Genes key to killer bee’s success | Next-generation sequencing (NGS) | Scoop.it

In a new study, researchers from Uppsala University sequenced the genomes of Africanized bees that have invaded large parts of the world to find out what makes them so extraordinarily successful. One particular region in the genome caught the researchers’ attention and the genes found there could be part of the explanation for the aggressive advances of these hybrid bees.

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Ageing: cell coordination breakdown 

Ageing: cell coordination breakdown  | Next-generation sequencing (NGS) | Scoop.it

Researchers from the European Bioinformatics Institute (EMBL-EBI) and the University of Cambridge have used single-cell sequencing to understand ageing.

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A DNA app store is here, but proceed with caution

A DNA app store is here, but proceed with caution | Next-generation sequencing (NGS) | Scoop.it

Helix will sequence your genes for $80 and lure app developers to sell you access to different parts of it.

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Unique, dual-matched adapters mitigate index hopping between NGS samples

The increasing throughput of NGS platforms has fueled the demand to sequence many samples in parallel, also referred to as multiplex sequencing. During multiplex sequencing, the identity of each sample library within a pool is maintained using index sequences that are subsequently separated in a process called demultiplexing during data analysis. Historically, a relatively small number of unique sequences (8 x i5 and 12 x i7) were used to create index combinations to multiplex samples. Unfortunately, with this combinatorial approach, a single index swap may cause a read to be mis-assigned to a different sample causing cross-talk. In this presentation, we discuss some sources of sample cross-talk, including index hopping during cluster amplification or multiplexed capture, and how index sequencing errors may lead to demultiplexing mistakes. We discuss how sample cross-talk causes demultiplexing errors and present a method for increasing the accuracy of sample identification using unique, dual-matched index adapters.

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First big efforts to sequence ancient African DNA reveal how early humans swept across the continent

First big efforts to sequence ancient African DNA reveal how early humans swept across the continent | Next-generation sequencing (NGS) | Scoop.it

The study of ancient human DNA has not been an equal opportunity endeavor. Early Europeans and Asians have had portions of their genomes sequenced by the hundreds over the past decade, rewriting Eurasian history in the process. But because genetic material decays rapidly in warm, moist climates, scientists had sequenced the DNA of just one ancient African. Until now.               

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A tiny DNA sequencer that’s traveled to space and the Antarctic can study life anywhere

A tiny DNA sequencer that’s traveled to space and the Antarctic can study life anywhere | Next-generation sequencing (NGS) | Scoop.it

A British company bets a hand-held analyzer will change how we look at DNA.

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New Genome Sequencing Method Helps Diagnose A Rare Genetic Condition 

New Genome Sequencing Method Helps Diagnose A Rare Genetic Condition  | Next-generation sequencing (NGS) | Scoop.it

When the race to sequence the human genome was reaching a fever pitch in the early 2000s, when I was in high school, I couldn’t help but wonder, “What comes next?” Once we had full access to our genetic blueprint, what more was there to do?

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$10 million DNA sequencing effort aims to shed light on lung diseases 

$10 million DNA sequencing effort aims to shed light on lung diseases  | Next-generation sequencing (NGS) | Scoop.it

Research is part of national project to understand genetic roots of heart, lung, blood, sleep disorders

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IDT partners with Illumina for NGS library preparation multiplexing and target enrichment

IDT partners with Illumina for NGS library preparation multiplexing and target enrichment | Next-generation sequencing (NGS) | Scoop.it

Leaders in NGS tools collaborate to deliver robust multiplexing capabilities and streamline target enrichment methods

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Sequencing of Green Alga Genome Provides Blueprint to Advance Clean Energy, Bioproducts 

Sequencing of Green Alga Genome Provides Blueprint to Advance Clean Energy, Bioproducts  | Next-generation sequencing (NGS) | Scoop.it

Scientists have sequenced the genome of a green alga that has drawn commercial interest as a strong producer of quality lipids for biofuel production. The chromosome-assembly genome of Chromochloris zofingiensis provides a blueprint for new discoveries in producing sustainable biofuels, antioxidants, and other valuable bioproducts.

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Analyzing the exome—focusing your NGS analysis with high performance target capture

As next generation sequencing has moved into the clinic, there is an increased demand for accuracy and reproducibility. Target enrichment is needed for applications where high read depth is critical, but some performance limitations, especially in GC-rich regions of the genome, have raised questions about the overall usefulness of target capture methods. In this webinar, Dr Kristina Giorda presents a method using individually synthesized and quality checked capture baits that performs well, even for GC-rich sequences, and delivers accurate coverage of the target space. Dr Giorda covers library preparation and target capture, and shares informative data generated using our xGen® Exome Research Panel.

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NGS detection of low frequency genetic variants using novel, molecular sequencing adaptors

NGS detection of low frequency genetic variants using novel, molecular sequencing adaptors | Next-generation sequencing (NGS) | Scoop.it

Watch our webinar recording to learn about unique molecular adaptors and a high-performance target capture method for NGS analysis of low frequency variants.

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Assessing the suitability of NGS panels for clinical sequencing 

Assessing the suitability of NGS panels for clinical sequencing  | Next-generation sequencing (NGS) | Scoop.it

Next generation sequencing (NGS) is beginning to live up to its promise in clinical diagnostics, but obtaining data that influences diagnostic decisions and patient outcomes requires products that deliver consistent, high-quality results. GMP (Good Manufacturing Practices)-compliant manufacturing is an indicator of such quality and consistency; however, clinical lab scientists still need to assess each new tool and product for clinical utility before integrating it into the workflow.

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Hunt for cancer 'tipping point' heats up

Hunt for cancer 'tipping point' heats up | Next-generation sequencing (NGS) | Scoop.it

'Pre-cancer' genome atlas proposed to track tumors as they turn from benign to dangerous.

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