DNA editing
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TeselaGen Is Building A Platform For Rapid Prototyping in Synthetic Biology - TechCrunch

TeselaGen Is Building A Platform For Rapid Prototyping in Synthetic Biology - TechCrunch | DNA editing | Scoop.it
TeselaGen has built visual tools that help researchers view and edit sequences, which is somewhat similar to what both Benchling and Genome Compiler do. But they’re also leveraging j5, which is a new software-based tool that automates DNA assembly and design.
Via Marko Dolinar
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Programmable DNA scissors for genome editing | KurzweilAI

Programmable DNA scissors for genome editing | KurzweilAI | DNA editing | Scoop.it
“ A new and possibly more effective way to edit genomes has discovered by an international team of scientists at Lawrence Berkeley National Laboratory.”
Via Zhiying Zhang
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A robust TALENs system for highly efficient mammalian genome editing - Sci. Reports

A robust TALENs system for highly efficient mammalian genome editing - Sci. Reports | DNA editing | Scoop.it
(via T. Lahaye, thx) Feng et al, 2013 Currently, for genomic editing in cultured cells, two plasmids encoding a pair of TALENs are co-transfected, followed by limited dilution to isolate cell colonies with the intended genomic manipulation. However, uncertain transfection efficiency becomes a bottleneck, especially in hard-to-transfect cells, reducing the overall efficiency of genome editing. We have developed a robust TALENs system in which each TALEN plasmid also encodes a fluorescence protein. Thus, cells transfected with both TALEN plasmids, a prerequisite for genomic editing, can be isolated by fluorescence-activated cell sorting. Our improved TALENs system can be applied to all cultured cells to achieve highly efficient genomic editing. Furthermore, an optimized procedure for genomic editing using TALENs is also presented. We expect our system to be widely adopted by the scientific community.
Via dromius, omni
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101 Uses of gBlocks® Gene Fragments

Providing a novel, rapid and reliable alternative to a full gene synthesis service, gBlocks® Gene Fragments are ideal for a large range of synthetic biology applications. As double-stranded, sequence verified DNA blocks of up to 750 base pairs, their high sequence fidelity in combination with rapid delivery times provide users with affordable and easy gene construction or modification.
Via Integrated DNA Technologies
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CRISPR technology leaps from lab to industry

CRISPR technology leaps from lab to industry | DNA editing | Scoop.it
Scientists launch company to develop the therapeutic potential of gene-snipping enzymes.
Via Integrated DNA Technologies
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CRISPR Creates Knockout Libraries

CRISPR Creates Knockout Libraries | DNA editing | Scoop.it
Two research groups have developed a database of human gene knockouts generated from the new genome editing technology.
Via Integrated DNA Technologies
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PAG XXII Workshop: gBlocks® Gene Fragments for Gene construction and more!

PAG XXII Workshop: gBlocks® Gene Fragments for Gene construction and more! | DNA editing | Scoop.it
Learn how gBlocks® Gene Fragments can be used for gene cloning, CRISPR-based genome modification, controls, and more at PAGXXII in San Diego on Monday, January 13th at 6:10pm. The workshop will be given by Adam Clore, Manager of the Synthetic Biology Design and Support group at IDT.
Via Integrated DNA Technologies
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Trends in Microbiology - RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing?

Trends in Microbiology - RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing? | DNA editing | Scoop.it

Via Steve Marek
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Randomized BioBrick Assembly: A Novel DNA Assembly Method for Randomizing and Optimizing Genetic Circuits and Metabolic Pathways

Randomized BioBrick Assembly: A Novel DNA Assembly Method for Randomizing and Optimizing Genetic Circuits and Metabolic Pathways | DNA editing | Scoop.it
The optimization of genetic circuits and metabolic pathways often involves constructing various iterations of the same construct or using directed evolution to achieve the desired function. Alternatively, a method that randomizes individual parts in the same assembly reaction could be used for optimization by allowing for the ability to screen large numbers of individual clones expressing randomized circuits or pathways for optimal function. Here we describe a new assembly method to randomize genetic circuits and metabolic pathways from modular DNA fragments derived from PCR-amplified BioBricks. As a proof-of-principle for this method, we successfully assembled CMY (Cyan-Magenta-Yellow) three-gene circuits using Gibson Assembly that express CFP, RFP, and YFP with independently randomized promoters, ribosome binding sites, transcriptional terminators, and all parts randomized simultaneously. Sequencing results from 24 CMY circuits with various parts randomized show that 20/24 circuits are distinct and expression varies over a 200-fold range above background levels. We then adapted this method to randomize the same parts with enzyme coding sequences from the lycopene biosynthesis pathway instead of fluorescent proteins, designed to independently express each enzyme in the pathway from a different promoter. Lycopene production is improved using this randomization method by about 40% relative to the highest polycistronic-expressing pathway. These results demonstrate the potential of generating nearly 20,000 unique circuit or pathway combinations when three parts are permutated at each position in a three-gene circuit or pathway, and the methodology can likely be adapted to other circuits and pathways to maximize products of interest.
Via Integrated DNA Technologies
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The power of CRISPR and genome engineering

The power of CRISPR and genome engineering | DNA editing | Scoop.it
“Clustered regularly interspaced short palindromic repeats."Catchy phrase, right? Well, no, not really. Which is why, in the proud tradition of science and tech, it was given an acronym: CRISPR. Which provides no further clue regarding what the phrase actually means, but is at least memorable. Clumsy naming aside, however, CRISPR is a big deal. But why? What is it and why is it beginning to cause something of a ruckus in genomics circles? And why, in news that just broke yesterday, are three venture capitalist firms backing a new venture, called Editas, to the tune of $43 million up front, to develop CRISPR-based gene-editing drugs?
Via Integrated DNA Technologies
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Accelerate Your Recombinant Gene or Protein Development

Accelerate Your Recombinant Gene or Protein Development | DNA editing | Scoop.it
NEW! Introducing gBlocks® Gene Fragments Libraries
Via Integrated DNA Technologies
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CRISPR and Cas9 for Flexible Genome Editing

CRISPR and Cas9 for Flexible Genome Editing | DNA editing | Scoop.it
CRISPR RNAs Make Targeted Gene Editing Easy
Via Integrated DNA Technologies
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Beyond Cloning: 101 Uses of Synthetic, High-Fidelity, Double-Stranded DNA

Beyond Cloning: 101 Uses of Synthetic, High-Fidelity, Double-Stranded DNA | DNA editing | Scoop.it
In addition to a standard gene synthesis service, IDT offers a novel, rapid, and reliable method to build and clone the genes you need at a fraction of the cost of full gene synthesis services.gBlocks® Gene Fragments are double-stranded, sequence-verified DNA blocks of length 125–750 bp. Their high sequence fidelity and rapid delivery time make gBlocks Gene Fragments ideal for a large range of synthetic biology applications. Dr Clore will review a variety of uses of gBlocks fragments, including CRISPR-based genome modification, qPCR and HRM controls, and the assembly of gene fragments using the Gibson Assembly® Method.
Via Integrated DNA Technologies
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