Diagnostic activities for plant pests
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Diagnostic activities for plant pests
Information on diagnostic activities on pests and diseases of plants follow the activities of EPPO Panels on diagnostics
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Detection of potato mop-top virus in soils and potato tubers using bait-plant bioassay, ELISA and RT-PCR

Detection of potato mop-top virus in soils and potato tubers using bait-plant bioassay, ELISA and RT-PCR | Diagnostic activities for plant pests | Scoop.it

Soil and plant samples from Northwest of Pakistan were collected from the region and tested for PMTV using both conventional and molecular techniques.The results indicate that a bait plant bioassay, infectivity assay, ELISA and RT-PCR can detect PMTV in roots and leaves of baited plants, field samples, zoospores of S. subterranea and tubers of 10 potato cultivars commercially grown in the region.

Petter Françoise's insight:

Potato mop-top virus is included in particular in  PM 4/28(1) Certification scheme for seed potatoes as well as in PM 3/21 (2) post entry quarantine for potato.

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EPPO Bulletin - Volume 43, Issue 3 - December 2013

EPPO Bulletin - Volume 43, Issue 3 - December 2013 | Diagnostic activities for plant pests | Scoop.it

The Diagnostic Protocols adopted at the last EPPO Council Session have now been published in the EPPO Bulletin December issue. 

EPPO Standards PM7 - Diagnostic protocols for regulated pestsInsects and mites
Bactrocera zonata (new)
Drosophila suzukii (new)
Tetranychus evansi (new)
Trogoderma granarium (revision PM 7/13)
Fungi
Hymenoscyphus pseudoalbidus (new)
Viruses
Tomato chlorosis virus and Tomato infectious chlorosis virus (new)Nematodes
Standard on nematode extraction (new)

Petter Françoise's insight:

Other EPPO Standards are also available in this issue

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MISIS: A bioinformatics tool to view and analyze maps of small RNAs derived from viruses and genomic loci generating multiple small RNAs

MISIS: A bioinformatics tool to view and analyze maps of small RNAs derived from viruses and genomic loci generating multiple small RNAs | Diagnostic activities for plant pests | Scoop.it

MISIS a tool to view and analyze sRNA maps of genomic loci and viruses which spawn multiple sRNAs. 

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U.S.: California researchers near HLB early detection | Fresh Fruit Portal

U.S.: California researchers near HLB early detection | Fresh Fruit Portal | Diagnostic activities for plant pests | Scoop.it
Research led by a University of California team could improve HLB treatment and diagnosis. (Research led by a University of California team could improve HLB treatment and diagnosis.
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Fraxinus: a Facebook game to crowdsource the fight against ash dieback disease HD

Please share and embed this video with credit to BBSRC. See full press release here: http://bit.ly/14Gxc2u Let scientists keep the lab coat, goggles and pipe...
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PaDIL high quality diagnostic images

PaDIL high quality diagnostic images | Diagnostic activities for plant pests | Scoop.it

Via Knapco
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Knapco's curator insight, September 17, 2013 10:15 AM
Australian Government offers PaDIL - a biodiversity and biosecurity information management system with delivering high quality diagnostic images. The infrastructure allows users to query datasets and to manage image database libraries. Use of the content is subject to terms and conditions.
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qPCR Experience® workshop: Real-time PCR in Plant pathology: Diagnostics and Research, Ljubljana, Slovenia, 3rd - 6th December 2013 | BioSistemika

qPCR Experience® workshop:

Real-time PCR in Plant pathology - Diagnostics and Research

 

Ljubljana, Slovenia, 3rd - 6th December 2013

 

 

Real-time PCR in Plant pathology will focus on diagnostics of plant pathogenic microorganisms and basics of relative quantification using real-time PCR integrating EPPO Guidelines.

 

Workshop is organised by National Institute of Biology (NIB) and BioSistemika Ltd.

 

Guest lecturers: Françoise Petter, EPPO and Sebastian Kiewnick, Agroscope.

Instructors: Dr. Tanja Dreo, Uni. Prof. Dr. Maja Ravnikar, Mag. Nataša Mehle,

Dr. Manca Pirc, Dr. Urška Čepin

 

The hands-on 4-day workshop will cover review of qPCR theory, LAMP technology, practical guidance experiment set-up,  plant sample preparation and quality control, assay design, automatisation, validation of diagnostic qPCR methods according to EPPO guidelines, as well as hands-on lab and data analysis sessions.

 

Extensive practical sessions will be held in ISO 9001 and ISO 17025 accredited laboratories of National Institute of Biology in Ljubljana which includestate-of-the-art qPCR centre. 

 

Join the international team of scientisis, experienced instructors and plant pathology specialists from the Department of Biotechnology and Systems Biology at National Institute of Biology.

 

Registrations and more information available here:  http://www.biosistemika.com/PP12_2013

 

Contact: info@biosistemika.com

 

Petter Françoise's insight:

New training session on real-time PCR. Previous sessions have attracted many participants. 

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A New Diagnostic System for Detection of ‘Candidatus Liberibacter asiaticus’ Infection in Citrus

A New Diagnostic System for Detection of ‘Candidatus Liberibacter asiaticus’ Infection in Citrus | Diagnostic activities for plant pests | Scoop.it
Plant Disease, Volume 97, Issue 10, Page 1295-1300, October 2013.

Two polyclonal antibodies were produced against the Omp protein of ‘Candidatus Liberibacter asiaticus’. The authors consider that these new antibodies will provide a very useful supplement to the current approaches to ‘Ca. L. asiaticus’ detection and also provide powerful research tools for tracking distribution of this pathogen in vivo.

Petter Françoise's insight:

An EPPO Diagnostic protocol is being developed on HLB

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Online diagnostic tools help USDA preserve, transfer knowledge

Online diagnostic tools help USDA preserve, transfer knowledge | Diagnostic activities for plant pests | Scoop.it
The Department of Agriculture's Animal and Plant Health Inspection Service is using online identification tools to prevent a loss of expertise and institutional knowledge caused by a growing number of people retiring

APHIS currently has about 50 tools available for download on IDTools.org. 

Petter Françoise's insight:

A number of identification tools for insects, plants.... 

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Specific primers for Xanthomonas vesicatoria, a tomato bacterial spot causal agent

Specific primers for Xanthomonas vesicatoria, a tomato bacterial spot causal agent | Diagnostic activities for plant pests | Scoop.it

These primers represent an additional tool for detection and identification of one of the species involved in this important disease complex.

Petter Françoise's insight:

An EPPO Protocol on Xanthomonas spp. (Xanthomonas euvesicatoria, Xanthomonas gardneri, Xanthomonas perforans, Xanthomonas vesicatoria) causing bacterial spot of tomato and sweet pepper (PM 7/110 (1) ) was adopted in 2012.

http://onlinelibrary.wiley.com/doi/10.1111/epp.12018/abstract;jsessionid=F7CD2A747C752DFA87C9F024443B062C.d02t03 ;

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Quantification of Live ‘Candidatus Liberibacter asiaticus’ Populations Using Real-Time PCR and Propidium Monoazide

Quantification of Live ‘Candidatus Liberibacter asiaticus’ Populations Using Real-Time PCR and Propidium Monoazide | Diagnostic activities for plant pests | Scoop.it

A rapid qPCR method that provides a more accurate way to determine GLB in HLB hosts which, in turn, should benefit disease epidemiology studies and serve as a crucial component in HLB management.

Petter Françoise's insight:

An EPPO diagnostic protocol is in preparation for Huanglongbing (HLB)

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National Plant Diagnostic Network Newsletter |

In the July issue of the NPDN newsletter you can find an article on the experience of alaboratory with the NPDN’s Laboratory Accreditation Program (STAR-D)

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EPPO Bulletin -August issue includes several articles of interest on diagnostics

EPPO Bulletin -August issue includes several articles of interest on diagnostics | Diagnostic activities for plant pests | Scoop.it

Read the 

Introduction to the QBOL-EPPO Conference on DNA barcoding and diagnostic methods for plant pests and several original articles 

 

Read the conclusions of the second Workshop for Heads of laboratories

Petter Françoise's insight:

 

 Most articles result from the QBOL-EPPO Conference.

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Loop-Mediated Amplification of the Clavibacter michiganensis subsp. michiganensis micA Gene Is Highly Specific

Loop-Mediated Amplification of the Clavibacter michiganensis subsp. michiganensis micA Gene Is Highly Specific | Diagnostic activities for plant pests | Scoop.it

Loop-mediated amplification (LAMP) was used to specifically identify Clavibacter michiganensis subsp. michiganensis, causal agent of bacterial canker of tomato. 

409 bacterial strains (351 C. michiganensis subsp.michiganensis and 58 non-C. michiganensis subsp. michiganensis) from a worldwide collection were tested with LAMP to determine its specificity. The LAMP reaction identified all C. michiganensis subsp.michiganensis strains and discriminated them from other C. michiganensis subspecies and non-Clavibacter bacteria.

This diagnostic tool has potential to provide an easy, one-step test for rapid identification of C. michiganensis subsp.michiganensis.

Petter Françoise's insight:

The last revised version of the  EPPO diagnostic protocol on Cmm PM 7/042 was published in April 2013) 

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Phytophthora Database 2.0: Update and Future Direction

Phytophthora Database 2.0: Update and Future Direction | Diagnostic activities for plant pests | Scoop.it

The online community resource Phytophthora database (PD) was developed to support accurate and rapid identification of Phytophthoraand to help characterize and catalog the diversity and evolutionary relationships within the genus. 

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9th Meeting of the Panel on Diagnostics in Nematology Poznan (PL), 2013-09-10/12

9th Meeting of the Panel on Diagnostics in Nematology  Poznan (PL), 2013-09-10/12 | Diagnostic activities for plant pests | Scoop.it
Petter Françoise's insight:

Read a short report of the last EPPO Panel on Nematology

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Potential of DNA Barcoding for Detecting Quarantine Fungi

Potential of DNA Barcoding for Detecting Quarantine Fungi | Diagnostic activities for plant pests | Scoop.it
Phytopathology, Volume 103, Issue 11, Page 1103-1107, November 2013.
Petter Françoise's insight:

An EU Project named QBOL was carried out for the development of a new diagnostic tool using DNA barcoding to identify quarantine organisms in support of plant health.A consortium of 20 partners (universities, research institutes and phytosanitary organizations) from all over the world working together and sharing their research expertise in the field of DNA barcoding of Arthropods,Bacteria, Fungi, Nematodes, Phytoplasmas and Viruses.

QBOL has been financed by the EU. QBOL makes collections harboring plant pathogenic quarantine organisms available. Informative genes from selected species on the EU Directive and EPPO lists are DNA barcoded. Sequences, together with taxonomic features, have been included in an internet-based Q-bank database.

 

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Occurrence of Meloidogyne fallax in North America, and Molecular Characterization of M. fallax and M. minor from U.S. Golf Course Greens

Occurrence of Meloidogyne fallax in North America, and Molecular Characterization of M. fallax and M. minor from U.S. Golf Course Greens | Diagnostic activities for plant pests | Scoop.it
Plant Disease, Volume 97, Issue 11, Page 1424-1430, November 2013.
Petter Françoise's insight:

Both Meloidogyne species are recommended for regulation by EPPO and a diagnostic protocl has been developed PM 7/41

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Molecular Plant Pathology: Top 10 plant-parasitic nematodes in molecular plant pathology (2013)

Molecular Plant Pathology: Top 10 plant-parasitic nematodes in molecular plant pathology (2013) | Diagnostic activities for plant pests | Scoop.it

The aim of this review was to undertake a survey of researchers working with plant-parasitic nematodes in order to determine a ‘top 10’ list of these pathogens based on scientific and economic importance. Any such list will not be definitive as economic importance will vary depending on the region of the world in which a researcher is based. However, care was taken to include researchers from as many parts of the world as possible when carrying out the survey. The top 10 list emerging from the survey is composed of: (1) root-knot nematodes (Meloidogyne spp.); (2) cyst nematodes (Heterodera and Globodera spp.); (3) root lesion nematodes (Pratylenchus spp.); (4) the burrowing nematode Radopholus similis; (5) Ditylenchus dipsaci; (6) the pine wilt nematode Bursaphelenchus xylophilus; (7) the reniform nematodeRotylenchulus reniformis; (8) Xiphinema index (the only virus vector nematode to make the list); (9) Nacobbus aberrans; and (10)Aphelenchoides besseyi. The biology of each nematode (or nematode group) is reviewed briefly.


Via Kamoun Lab @ TSL, nematodes
Petter Françoise's insight:

many are recommended for regulation by EPPO

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DNA tech innovations could help solve potato scab - FarmersWeekly

DNA tech innovations could help solve potato scab - FarmersWeekly | Diagnostic activities for plant pests | Scoop.it
DNA tech innovations could help solve potato scab
FarmersWeekly
New technology for identifying diseases and harnessing the plant's own defences are two key ways scientists hope to help growers protect their crops in the coming years.
Petter Françoise's insight:

EPPO and the EU project Q-detect organized a workshop for inspectors on onsite diagnostics

http://archives.eppo.int/MEETINGS/2013_conferences/inspectors_slovenia.htm

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Identification of a New Enamovirus Associated with Citrus Vein Enation Disease by Deep Sequencing of Small RNAs

Identification of a New Enamovirus Associated with Citrus Vein Enation Disease by Deep Sequencing of Small RNAs | Diagnostic activities for plant pests | Scoop.it

Dot-blot hybridization and reverse transcription-polymerase chain reaction tests have been developed to detect CVEV in plants affected by vein enation disease. CVEV detection by these methods has already been adopted for use in the Spanish citrus quarantine, sanitation, and certification programs.

 
Petter Françoise's insight:

Citrus vein enation  is icnluded in the EPPO standard PM 4/12 certification schemes pathogen-tested citrus trees and rootstocks, however only indexing on mexican lime is recommended for detection. 

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Current situation And Strain Characterization Of Pseudomonas syringae pv. Actinidae On Kiwifruit In Galicia

Current situation And Strain Characterization Of Pseudomonas syringae pv. Actinidae On Kiwifruit In Galicia | Diagnostic activities for plant pests | Scoop.it

The analysis of phytotoxin production-related cfl and tox-argK cluster gene and the phylogenetic analysis of the cts gene demonstrate that all Psa strains from NW Spain correspond to Psa3 population, which includes strains of haplotype 2. This is the first characterization of Psa3 and haplotype 2 in Spain.

Petter Françoise's insight:

An EPPO diagnostic protocol is in preparation for this bacterium. 

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Development of Primers and Probes for Genus and Species Specific Detection of ‘Candidatus Liberibacter Species’ by Real-Time PCR

Development of Primers and Probes for Genus and Species Specific Detection of ‘Candidatus Liberibacter Species’ by Real-Time PCR | Diagnostic activities for plant pests | Scoop.it

The most widely used tests use primers and probes based on the 16S ribosomal RNA (rRNA) gene. The 16S rRNA-based tests to detect the three species are species-specific and must be performed sequentially. In this article a qPCR diagnostic test useful for the detection of all species of Liberibacter infecting citrus is described. In addition, the degenerate genus-specific primers and probe successfully detected ‘Ca. Liberibacter solanacearum’, a psyllid-transmitted pathogen associated with disease in tomato, carrot, and potato.

Petter Françoise's insight:

An EPPO Diagnostic protocl on HLB is under development 

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Characterization of Sour Cherry Isolates of Plum pox virus from the Volga Basin in Russia Reveals a New Cherry Strain of the Virus

Characterization of Sour Cherry Isolates of Plum pox virus from the Volga Basin in Russia Reveals a New Cherry Strain of the Virus | Diagnostic activities for plant pests | Scoop.it

Three complete and several partial genomic sequences of PPV isolates from sour cherry trees in the Volga River basin of Russia have been determined. 


A specific primer pair has been developed, allowing the specific reverse-transcription polymerase chain reaction detection of PPV-CR isolates.

Petter Françoise's insight:

PPV is an EPPO A2 Listed pest and an EPPO protocol is under revision to align it to the IPPC diagnostic protocol adopted in 2012. 

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ISTA Seed Health Committee (SHC) seeking feedback on the current Method on Aphelenchoides besseyi for Oryza sativa seeds.

ISTA Seed Health Committee (SHC) seeking feedback on the current Method on Aphelenchoides besseyi for Oryza sativa seeds. | Diagnostic activities for plant pests | Scoop.it

MESSAGE FROM ISTA Seed Health Committee

Dear Colleague

ISTA Seed Health Committee Official Method Review

The Seed Health Method Validation Programme requires that methods approved as Official Methods should be reviewed every 5 years to ensure their continuing effectiveness and suitability. Official Seed Health Testing Methods are found in the International Rules for Seed Testing 2013. There is currently one ISTA Official Seed Health Testing Methods that is now due for review (listed in Table 1). This is the first review for Method 7-025. The ISTA Seed Health Committee (SHC) would like to receive your feedback on the current Method Sheet as part of the review process.

Table 1. The one ISTA Official Seed Health Testing Method to be reviewed in 2013

To help us we would be pleased if you could complete the online questionnaire by 15 October 2013.

Link to online questionnaire.

https://docs.google.com/forms/d/1rZ97NAkXyHVKEstUCKQSHzhFLpmzxOdjZmAc4dK8hW4/viewform

YOUR HELP WITH THIS REVIEW IS MUCH APPRECIATED.

Please feel free to ignore this message, if your lab does not conduct any seed health test.

With best wishes

Nadine Ettel
Technical Committee Coordinator
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International Seed Testing Association (ISTA)
Zuerichstrasse 50, 8303 Bassersdorf, CH-Switzerland
Phone: +41 44 838 60 00, Fax: +41 44 838 60 01
E-mail: ista.office@ista.ch, www.seedtest.org
 

Petter Françoise's insight:

EPPO has a diagnostic protocol on A besseyi (PM 7/39) which is refered to in the ISTA method.

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