Diagnostic activi...
Follow
Find
13.7K views | +8 today
 
Scooped by Petter Françoise
onto Diagnostic activities for plant pests
Scoop.it!

A new session of the qPCR Experience® workshop: Real-time PCR in Plant pathology is planned in April 2013

A new session of the qPCR Experience® workshop: Real-time PCR in Plant pathology is planned in April 2013 | Diagnostic activities for plant pests | Scoop.it

As there was a lot of interest expressed for the first Real-time PCR workshop in Plant pathology consequently an extra workshop will be organized to be held in Ljubljana, Slovenia, 9th - 12th April 2013.

The focus of this workshop will be on diagnostics and quantification of plant pathogenic microorganisms using real-time PCR.

 

For more information and registration please visit: http://www.biosistemika.com/PlantPathology2013

more...
No comment yet.
Diagnostic activities for plant pests
Information on diagnostic activities on pests and diseases of plants follow the activities of EPPO Panels on diagnostics
Your new post is loading...
Your new post is loading...
Scooped by Petter Françoise
Scoop.it!

EPPO and Diagnostics for plant pests of quarantine significance

EPPO and Diagnostics for plant pests of quarantine significance | Diagnostic activities for plant pests | Scoop.it

 

'Diagnostic activities for plant pests' is maintained by the Secretariat of the European and Mediterranean Plant Protection Organization (EPPO) and its aim is to share information on diagnostics.

 

EPPO is an intergovernmental organization created in 1951 which currently has 50 member countries. EPPO is responsible for harmonization and cooperation among the National Plant Protection Organizations (official authorities) of its member countries. EPPO helps its members in their efforts to protect plant health in agriculture, forestry and the uncultivated environment (standard-setting activities and exchange of information).

 

EPPO has established a programme on diagnostics since 1992

view the approved diagnostic protocols on the EPPO official website:

http://archives.eppo.int/EPPOStandards/diagnostics.htm

 

On this website, a page dedicated to EPPO activities on diagnostics is also available at: http://www.eppo.int/QUARANTINE/diagnostic_activities.htm

Petter Françoise's insight:

The EPPO Secretariat maintains other scoop.it magazines onPest Risk Analysis (http://www.scoop.it/t/pest-risk-analysis) Pest Alerts (http://www.scoop.it/t/pest-alerts), Invasive Alien Plants (http://www.scoop.it/t/invasive-alien-plants), Video of plant pests (http://www.scoop.it/t/pests-on-videos), Communication on pests (http://www.scoop.it/t/communication-and-citizen-sciences-on-pests-and-invasive-alien-species)

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

New EPPO Diagnotic protocols published- EPPO Bulletin -

New EPPO Diagnotic protocols published- EPPO Bulletin - | Diagnostic activities for plant pests | Scoop.it
Petter Françoise's insight:

PM 7/123 (1) Phytophthora lateralis 
PM 7/124 (1) Spodoptera littoralis, Spodoptera litura, Spodoptera frugiperda, Spodoptera eridania 
PM 7/125 (1) ELISA tests for viruses 
PM 7/126 (1) Electron microscopy in diagnosis of plant viruses

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Inter-laboratory comparison of four RT-PCR based methods for the generic detection of pospiviroids in tomato leaves and seeds - Online First - Springer

Inter-laboratory comparison of four RT-PCR based methods for the generic detection of pospiviroids in tomato leaves and seeds - Online First - Springer | Diagnostic activities for plant pests | Scoop.it

Four pospiviroid detection methods
consisting of a pair of RT-PCR (ANSES 1-2), a pair of
real time RT-PCR (Botermans 1-2) and two single RTPCR
methods (Luigi and Olivier) were evaluated for
their relative accuracy, diagnostic sensitivity, diagnostic
specificity, analytical sensitivity and reproducibility
through a comparison in eight laboratories. All detection
methods were tested on 13 tomato leaf and nine tomato
seed samples as well as on 100-fold dilutions of the
corresponding RNA extracts. Two different RNA extraction
kits and three combinations of RNA extraction
and RT-PCR kits were assessed. According to the statistical
analysis of the results, ANSES 1-2 method provided
the best performance criteria whatever the matrix

Petter Françoise's insight:

An EPPO  diagnostic protocol on the generic detection of pospiviroids is in preparation 

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Hymenoscyphus fraxineus mycelial growth on media containing leaf extracts of different Oleaceae - Carrari et al 2015

Hymenoscyphus fraxineus mycelial growth on media containing leaf extracts of different Oleaceae - Carrari et al 2015 | Diagnostic activities for plant pests | Scoop.it

We assessed the mycelial growth rate of Hymenoscyphus fraxineus, the causal agent of ash dieback, on agar media containing leaf extracts of seven common Mediterranean species of the Oleaceae (Fraxinus excelsior, F. angustifolia, F. ornus, Ligustrum vulgare, Olea europaea, Phyllirea latifolia and Syringa vulgaris). The pathogen grew on all media, but growth rates showed significant differences among media and H. fraxineus isolates. Growth rates were highest on media containing F. excelsior and F. angustifolia, intermediate on media containing O. europaea and P. latifolia and lowest on those containing F. ornus and L. vulgare

Petter Françoise's insight:

See also the EPPO Diagnostic protocol on this pest

http://onlinelibrary.wiley.com/enhanced/doi/10.1111/epp.12061/ ;

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

2nd Q-collect project Workshop

2nd Q-collect project Workshop | Diagnostic activities for plant pests | Scoop.it
A Workshop for plant pest collections and for users of biological material -
Rome, 2015-09-08/09
Petter Françoise's insight:

Read a summary of the meeting and conclusions. 

A white paper on collections was also finalized during this meeting. 

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Detection of Citrus leprosis virus C using specific primers and TaqMan probe in one-step real-time reverse-transcription polymerase chain reaction assays

Detection of Citrus leprosis virus C using specific primers and TaqMan probe in one-step real-time reverse-transcription polymerase chain reaction assays | Diagnostic activities for plant pests | Scoop.it
Citrus leprosis virus C
more...
No comment yet.
Suggested by Anne-Sophie Roy
Scoop.it!

LepIntercept: an identification resource for intercepted Lepidoptera larvae

LepIntercept: an identification resource for intercepted Lepidoptera larvae | Diagnostic activities for plant pests | Scoop.it
Petter Françoise's insight:

A useful ressource!

more...
Jorge Luiz's curator insight, August 3, 2015 6:27 AM

A useful ressource!

Scooped by Petter Françoise
Scoop.it!

Improved morphological key to the species of the xylophilus group of the genus Bursaphelenchus Fuchs, 1937 - Braasch & Schönfeld 2015

Improved morphological key to the species of the xylophilus group of the genus Bursaphelenchus Fuchs, 1937 - Braasch & Schönfeld 2015 | Diagnostic activities for plant pests | Scoop.it
Correct identification of the pine wood nematode Bursaphelenchus xylophilus becomes more difficult, as more related or similar species are described. Spread of the nematode is being monitored worldwide, and in view of the fact that several new Bursaphelenchus species have been described, identification keys should be adjusted accordingly. Based on the identification key of the xylophilus group published by Braasch (2008) and the revised intrageneric grouping of Bursaphelenchus by Braasch et al. (2009), an improved dichotome morphological key including newly described species (B. paraluxuriosae, B. populi, B. macromucronatus, B. firmae, B. koreanus and B. gillanii) is presented. The recognition of the quarantine pest B. xylophilus using morphological characteristics in laboratories of the National Plant Protection Services is facilitated by a simplified key
more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Detection and identification of Xanthomonas arboricola pv. pruni from symptomless plant material: results of an Italian test performance study - Loreti et al 2015 -

Detection and identification of Xanthomonas arboricola pv. pruni from symptomless plant material: results of an Italian test performance study - Loreti et al 2015 - | Diagnostic activities for plant pests | Scoop.it
anthomonas arboricola pv. pruni, the causal agent of bacterial spot disease of stone fruits, is a regulated quarantine pathogen in the European Union, listed as an A2 pest by the European and Mediterranean Plant Protection Organization (EPPO). Because detection and identification of this pathogen is key for its management and to ensure the production of pest free propagation material, it should be based on reliable tests, in particular when dealing with symptomless material. The current EPPO diagnostic Standard (PM 7/64) does not provide specific molecular methods for detection of this pest. The present paper summarizes the results of a test-performance study (TPS) to validate, at a national level, a detection procedure for this bacterium. A working group was established in order to evaluate the performance criteria for tests included in the current EPPO Standard, and for a conventional PCR. On the basis of the obtained performance criteria, a diagnostic procedure was elaborated and then applied to perform an inter-laboratory comparison. Screening tests for the detection of the bacterium on symptomless plant material based on IF and/or PCR were proposed, in parallel with isolation on agar media. For identification two methods were suggested: a molecular test or IF. This paper reports on the results of the TPS and proposes a flow diagram for the detection and identification of X. arboricola pv. pruni.
Petter Françoise's insight:

The EPPO protocol on Xanthomonas arboricola pv. pruni is under revision

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Detection of PSTVd and TCDVd in seeds of tomato using real-time RT-PCR - Bakker et al. 2015

Detection of PSTVd and TCDVd in seeds of tomato using real-time RT-PCR - Bakker et al. 2015 | Diagnostic activities for plant pests | Scoop.it
Worldwide outbreaks of pospiviroids in potato and tomato have increased the need for a reliable test for the detection of pospiviroids in seeds. This study describes the development and validation of a sensitive and fast test for the detection of Potato spindle tuber viroid (PSTVd) and Tomato chlorotic dwarf viroid (TCDVd) in tomato seeds. The test is based on RNA isolation using a commercial kit and is suitable for routine application. The test is able to detect one PSTVd or TCDVd contaminated seed in sub samples of 1000 seeds and results were both repeatable and reproducible.
Petter Françoise's insight:

The EPPO Protocol on PSTVd is under revision and a standard on generic detection of pospiviroids is in preparation.

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

A quantitative hybridization approach using seventeen DNA markers for identification and clustering analysis of Ralstonia solanacearum - Albuquerque - Plant Pathology - Wiley Online Library

A quantitative hybridization approach using seventeen DNA markers for identification and clustering analysis of Ralstonia solanacearum - Albuquerque - Plant Pathology - Wiley Online Library | Diagnostic activities for plant pests | Scoop.it
Ralstonia solanacearum (Rs) is a quarantine phytopathogenic bacterium accountable for heavy economic losses worldwide. Monitoring and eradication programs required for this pathogen are dependent on the availability of time- and cost-efficient detection and typing methods. However, members of the Rs species complex are characterized by a high phenotypic and genetic diversity, which requires improved diagnostics methods.

The currently available full genome sequences of several Rs strains allow for the selection of novel specific DNA markers using comparative genomics tools. In this work, seventeen novel markers were selected based on Rs-specific protein domains and thoroughly validated for specificity and stability, both in silico and using “wet lab” assays. PCR- and hybridization-based validation assays revealed that the DNA regions selected as markers were unevenly distributed amongst the tested strains, with nine markers present throughout the species complex. The distribution of the remaining eight markers was highly variable between the different analyzed strains and allowed to obtain strain-specific dot blot hybridization patterns particularly informative for typing. The average probability value of each strain being positive for each of the seventeen markers was calculated by an algorithm and used to obtain a dendrogram representing the hierarchical clustering analysis of Rs, according to the similarity of their hybridization patterns. We believe that this method can be a robust contribution for the straightforward genotyping of members from the Rs species complex. Furthermore, this quantitative hybridization approach would allow to construct databases increasingly informative to determine new Rs genotypes and infer epidemiological patterns.
Petter Françoise's insight:

The EPPO Diagostic protocol for Ralstona solanacearum is currently under revision

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Transmission of ‘Candidatus Liberibacter solanacearum’ in carrot seeds - Bertolini - 2014 - Plant Pathology - Wiley Online Library

Transmission of ‘Candidatus Liberibacter solanacearum’ in carrot seeds - Bertolini - 2014 - Plant Pathology - Wiley Online Library | Diagnostic activities for plant pests | Scoop.it
A protocol for the specific detection and quantification of ‘Candidatus Liberibacter solanacearum’ in carrot seeds using real-time PCR was developed. The bacterium was detected in 23 out of 54 carrot seed lots from 2010 to 2014, including seeds collected from diseased mother plants. The average total number of ‘Ca. L. solanacearum’ cells in individual seeds ranged from 4·8 ± 3·3 to 210 ± 6·7 cells per seed from three seed lots, but using propidium monoazide to target live cells, 95% of the cells in one seed lot were found to be dead. Liberibacter-like cells were observed in the phloem sieve tubes of the seed coat and in the phloem of carrot leaf midrib from seedlings. The bacterium was detected as early as 30 days post-germination, but more consistently after 90 days, in seedlings grown from PCR positive seed lots in an insect-proof P2 level containment greenhouse. Between 12% and 42% of the seedlings from positive seed lots tested positive for ‘Ca. L. solanacearum’. After 150 days, symptoms of proliferation were observed in 12% of seedlings of cv. Maestro. ‘Candidatus Liberibacter solanacearum’ haplotype E was identified in the seeds and seedlings of cv. Maestro. No phytoplasmas were detected in seedlings with symptoms using a real-time assay for universal detection of phytoplasmas. The results show that to prevent the entry and establishment of the bacterium in new areas and its potential spread to other crops, control of ‘Ca. L. solanacearum’ in seed lots is required.
Petter Françoise's insight:

An EPPO diagnostic protocol on Candidatus Liberibacter solanacearum is in preparation

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Testa - EPPO Conference on diagnostics for plant pests

Testa - EPPO Conference on diagnostics for plant pests | Diagnostic activities for plant pests | Scoop.it

The EPPO-TESTA Conference was organized in Angers on 2015-11-30/12-02 at the kind invitation of the Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail (ANSES, FR) and of the Groupe d'Etude et de contrôle des Variétés et des Semences (GEVES, FR)

Petter Françoise's insight:

Read the summary of the meeting and the main conclusions and recommendations

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Assessment of a new qPCR tool for the detection and identification of the root-knot nematode Meloidogyne enterolobii by an international test performance study - Springer

Assessment of a new qPCR tool for the detection and identification of the root-knot nematode Meloidogyne enterolobii by an international test performance study - Springer | Diagnostic activities for plant pests | Scoop.it
more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Simultaneous detection of Clavibacter michiganensis subsp. michiganensis, Pepino mosaic virus and Mexican papita viroid by non-radioactive molecular hybridization using a unique polyprobe - Springer

Simultaneous detection of Clavibacter michiganensis subsp. michiganensis, Pepino mosaic virus and Mexican papita viroid by non-radioactive molecular hybridization using a unique polyprobe - Springer | Diagnostic activities for plant pests | Scoop.it

Clavibacter michiganensis subsp. michiganensis, Pepino mosaic virus, and Mexican papita viroidare three economically important pathogens that infect tomato crops. In this work, a polyprobe (poly-3) was developed and evaluated for the simultaneous detection of these pathogens in tomato plants by non-isotopic molecular hybridization. The endpoint detection limit of the poly-3 with C. michiganensis subsp. michiganensis cell cultures was 106 CFU/ml. No differences were found in terms of the sensitivity and specificity when the individual riboprobes were compared to the poly-3 for the detection of the three pathogens. The analysis of 80 tomato field samples by the poly-3 and RT-PCR techniques rendered the same number of positive samples for each pathogen. As far as we know this is the first time that three pathogens with very different life cycle styles (bacteria, virus and viroid) are simultaneously detected in a single assay. The possibility of using this poly-3 technology for the routine diagnosis of field tomato samples is discussed.

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

3rd Workshop for Heads of Laboratories Rome, 2015-09-08/11

3rd Workshop for Heads of Laboratories Rome, 2015-09-08/11 | Diagnostic activities for plant pests | Scoop.it
Petter Françoise's insight:

View the presentations and read the summary and conclusions of the Workshop

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

A real-time TaqMan PCR assay to discriminate between pathotype 1 (D1) and non-pathotype 1 (D1) isolates of Synchytrium endobioticum - Springer

A real-time TaqMan PCR assay to discriminate between pathotype 1 (D1) and non-pathotype 1 (D1) isolates of Synchytrium endobioticum - Springer | Diagnostic activities for plant pests | Scoop.it
Petter Françoise's insight:

The EPPO Diagnostic Protocol on Synchytrium endobioticum is under revision and will be studied at the next meeting of the PAnel on DIagnostics in Mycology in November 2015.

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Development and Validation of LNA-Based Quantitative Real-Time PCR Assays for Detection and Identification of the Root-Knot Nematode Meloidogyne enterolobii in Complex DNA Backgrounds

Development and Validation of LNA-Based Quantitative Real-Time PCR Assays for Detection and Identification of the Root-Knot Nematode Meloidogyne enterolobii in Complex DNA Backgrounds | Diagnostic activities for plant pests | Scoop.it
Phytopathology, Ahead of Print.
Petter Françoise's insight:

An EPPO diagnostic protocol on Meloidogyne enterolobii PM 7/103 is under revision.  

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Two revised EPPO diagnostic protocols published in the EPPO Bulletin

Two revised EPPO diagnostic protocols published in the EPPO Bulletin | Diagnostic activities for plant pests | Scoop.it

PM 7/46 (3) Lecanosticta acicola (formerly Mycosphaerella dearnessii),Dothistroma septosporum

PM 7/048 (3) Plenodomus tracheiphilus 

Petter Françoise's insight:

Two revised EPPO diagnostic protocols published and available freely online.

 

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

9th Meeting of the Panel on Diagnostics in Nematology

9th Meeting of the Panel on Diagnostics in Nematology | Diagnostic activities for plant pests | Scoop.it

Read a short report of the 9th Meeting of the Panel on Diagnostics in Nematology 

Petter Françoise's insight:

Keep updated with the EPPO programme of preparation of diagnostic protocols in nematology

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

3rd Meeting of the Panel on Diagnostics in Entomology

3rd Meeting of the Panel on Diagnostics in Entomology | Diagnostic activities for plant pests | Scoop.it

Short report of the 3rd Meeting of the Panel on Diagnostics in Entomology. 

Petter Françoise's insight:

Keep updated with the EPPO programme of preparation of diagnostic protocols in entomology

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Comparison of different PCR tests to identify Monilinia fructicola - Riccioni & Valente 2015

Comparison of different PCR tests to identify Monilinia fructicola - Riccioni & Valente 2015 | Diagnostic activities for plant pests | Scoop.it
Monilinia fructicola was until very recently a regulated pest in the European Union, and EU countries were requested to monitor its presence on their territories. As accredited laboratories should use validated tests, the mycological laboratory of CRA-PAV carried out a validation process for the multiplex based PCR test (Coté et al., 2004), that is one of the most widely used tests for the identification of M. fructicola, although this test is not described in the EPPO diagnostic protocol PM 7/18 (2) because the validation data were lacking. The performance characteristics of this multiplex PCR test were established according to the EPPO Standard PM 7/98 (1) and the test was compared in a collaborative study with the end point PCR test (Ioos & Frey, 2000), considered as the ‘standard test’. The validation data were obtained using different isolates of M. fructicola, M. laxa, M. fructigena and Monilia polystroma, as well as different fruit tissues. Four series of the DNA target at different concentration, repeated three times, were analyzed in four Italian laboratories. The results showed that the multiplex PCR detection test (Coté et al., 2004) was fit for diagnostic purpose, although the analytical sensitivity was significantly lower compared to the conventional PCR ‘standard test’.
Petter Françoise's insight:

An EPPO Protoocl on M fructicola is under revision

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Detection of the Quarantine Species Thrips palmi by Loop-Mediated Isothermal Amplification

Detection of the Quarantine Species Thrips palmi by Loop-Mediated Isothermal Amplification | Diagnostic activities for plant pests | Scoop.it
Thrips palmi (from the order Thysanoptera) is a serious insect pest of various crops, including vegetables, fruits and ornamental plants, causing significant economic losses. Its presence constitutes a double threat; not only does T. palmi feed on the plants, it is also a vector for several plant viruses. T. palmi originated in Asia, but has spread to North and Central America, Africa, Oceania and the Caribbean in recent decades. This species has been sporadically noted in Europe and is under quarantine regulation in the European Union. For non-specialists its larval stages are indistinguishable morphologically from another widespread and serious insect pest Frankliniella occidentalis (a non-quarantine species in the European Union) as well as other frequently occurring thrips. In this study, we have developed a loop-mediated isothermal amplification protocol to amplify rDNA regions of T. palmi. The results were consistent whether isolated DNA or crushed insects were used as template, indicating that the DNA isolation step could be omitted. The described method is species-specific and sensitive and provides a rapid diagnostic tool to detect T. palmi in the field.
Petter Françoise's insight:

An IPPC protocol has been published in this pest 

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

LAMP assay and rapid sample preparation method for on-site detection of flavescence dorée phytoplasma in grapevine - Kogovšek - 2014 - Plant Pathology - Wiley Online Library

LAMP assay and rapid sample preparation method for on-site detection of flavescence dorée phytoplasma in grapevine - Kogovšek - 2014 - Plant Pathology - Wiley Online Library | Diagnostic activities for plant pests | Scoop.it
In Europe the most devastating phytoplasma associated with grapevine yellows (GY) diseases is a quarantine pest, flavescence dorée (FDp), from the 16SrV taxonomic group. The on-site detection of FDp with an affordable device would contribute to faster and more efficient decisions on the control measures for FDp. Therefore, a real-time isothermal LAMP assay for detection of FDp was validated according to the EPPO standards and MIQE guidelines. The LAMP assay was shown to be specific and extremely sensitive, because it detected FDp in all leaf samples that were determined to be FDp infected using quantitative real-time PCR. The whole procedure of sample preparation and testing was designed and optimized for on-site detection and can be completed in one hour. The homogenization procedure of the grapevine samples (leaf vein, flower or berry) was optimized to allow direct testing of crude homogenates with the LAMP assay, without the need for DNA extraction, and was shown to be extremely sensitive.
Petter Françoise's insight:

A revision of the EPPO Protocol on Flavescence dorée is in preparation.

more...
No comment yet.
Scooped by Petter Françoise
Scoop.it!

Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples

Real-time PCR and LAMP were suitable for reliable, sensitive X. arboricola pv. pruni detection.

The first technique was slightly more accurate than LAMP, depending upon sample preparation.

Critical data to support recommendations for practical use and phytosanitary inspection guidelines are provided.
Petter Françoise's insight:

A revision of the EPPO protocol onX. arboricola pv. pruni is in preparation and hsould be presented at the next Panel on Diagnostics in Bacteriology in October 2015

more...
No comment yet.