Diagnostic activities for plant pests
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Diagnostic activities for plant pests
Information on diagnostic activities on pests and diseases of plants follow the activities of EPPO Panels on diagnostics
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EPPO and Diagnostics for plant pests of quarantine significance

EPPO and Diagnostics for plant pests of quarantine significance | Diagnostic activities for plant pests | Scoop.it

 

'Diagnostic activities for plant pests' is maintained by the Secretariat of the European and Mediterranean Plant Protection Organization (EPPO) and its aim is to share information on diagnostics.

 

EPPO is an intergovernmental organization created in 1951 which currently has 50 member countries. EPPO is responsible for harmonization and cooperation among the National Plant Protection Organizations (official authorities) of its member countries. EPPO helps its members in their efforts to protect plant health in agriculture, forestry and the uncultivated environment (standard-setting activities and exchange of information).

 

EPPO has established a programme on diagnostics since 1992

view the approved diagnostic protocols on the EPPO official website:

http://archives.eppo.int/EPPOStandards/diagnostics.htm

 

On this website, a page dedicated to EPPO activities on diagnostics is also available at: http://www.eppo.int/QUARANTINE/diagnostic_activities.htm

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The EPPO Secretariat maintains other scoop.it magazines onPest Risk Analysis (http://www.scoop.it/t/pest-risk-analysis) Pest Alerts (http://www.scoop.it/t/pest-alerts), Invasive Alien Plants (http://www.scoop.it/t/invasive-alien-plants), Video of plant pests (http://www.scoop.it/t/pests-on-videos), Communication on pests (http://www.scoop.it/t/communication-and-citizen-sciences-on-pests-and-invasive-alien-species)

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Euroreference

Euroreference | Diagnostic activities for plant pests | Scoop.it
The Euroreference magazine is now available in a new formula, produced jointly by a group of agencies from several Member States of the European Union. Euroreference is a European journal created by ANSES in 2009. It deals specifically with reference activities in the areas of animal health, plant health, and food and drinking water safety. Its new formula is based on an editorial committee of 15 European partners, making the new Euroreference a collective publication devoted to better dissemination of knowledge at the European and international scales. It offers scientific and technical articles of interest to health protection laboratories and agencies involved in reference activities in Europe. Euroreference is thus designed to help enhance efficiency in European reference activities.
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Generic RT-PCR tests for detection and identification of tospoviruses

A set of tests for generic detection and identification of tospoviruses has been developed. Based on a multiple sequence alignment of the nucleocapsid gene and its 5′ upstream untranslated region sequence from 28 different species, primers were designed for RT-PCR detection of tospoviruses from all recognized clades, i.e. the American, Asian and Eurasian clades, and from the small group of distinct and floating species. Pilot experiments on isolates from twenty different species showed that the designed primer sets successfully detected all species by RT-PCR, as confirmed by nucleotide sequence analysis of the amplicons. In a final optimized design, the primers were applied in a setting of five RT-PCR tests. Seven different tospoviruses were successfully identified from diagnostic samples and in addition a non-described tospovirus species from alstroemeria plants. The results demonstrate that the newly developed generic RT-PCR tests provide a relevant tool for broad detection and identification of tospoviruses in plant quarantine and diagnostic laboratories.
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An EPPO Diagnostic protocl on generic detection of tospoviruses is in preparation (it will also include specifc tospoviruses)
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Short report of the last EPPO Panel on Diagnostics and Quality Assurance

Short report of the last EPPO Panel on Diagnostics and Quality Assurance | Diagnostic activities for plant pests | Scoop.it
Read this short report to learn about the topics discussed during the last Panel 
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New EPPO Diagnotic protocols published- EPPO Bulletin -

New EPPO Diagnotic protocols published- EPPO Bulletin - | Diagnostic activities for plant pests | Scoop.it
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PM 7/123 (1) Phytophthora lateralis 
PM 7/124 (1) Spodoptera littoralis, Spodoptera litura, Spodoptera frugiperda, Spodoptera eridania 
PM 7/125 (1) ELISA tests for viruses 
PM 7/126 (1) Electron microscopy in diagnosis of plant viruses

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Inter-laboratory comparison of four RT-PCR based methods for the generic detection of pospiviroids in tomato leaves and seeds - Online First - Springer

Inter-laboratory comparison of four RT-PCR based methods for the generic detection of pospiviroids in tomato leaves and seeds - Online First - Springer | Diagnostic activities for plant pests | Scoop.it

Four pospiviroid detection methods
consisting of a pair of RT-PCR (ANSES 1-2), a pair of
real time RT-PCR (Botermans 1-2) and two single RTPCR
methods (Luigi and Olivier) were evaluated for
their relative accuracy, diagnostic sensitivity, diagnostic
specificity, analytical sensitivity and reproducibility
through a comparison in eight laboratories. All detection
methods were tested on 13 tomato leaf and nine tomato
seed samples as well as on 100-fold dilutions of the
corresponding RNA extracts. Two different RNA extraction
kits and three combinations of RNA extraction
and RT-PCR kits were assessed. According to the statistical
analysis of the results, ANSES 1-2 method provided
the best performance criteria whatever the matrix

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An EPPO  diagnostic protocol on the generic detection of pospiviroids is in preparation 

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Hymenoscyphus fraxineus mycelial growth on media containing leaf extracts of different Oleaceae - Carrari et al 2015

Hymenoscyphus fraxineus mycelial growth on media containing leaf extracts of different Oleaceae - Carrari et al 2015 | Diagnostic activities for plant pests | Scoop.it

We assessed the mycelial growth rate of Hymenoscyphus fraxineus, the causal agent of ash dieback, on agar media containing leaf extracts of seven common Mediterranean species of the Oleaceae (Fraxinus excelsior, F. angustifolia, F. ornus, Ligustrum vulgare, Olea europaea, Phyllirea latifolia and Syringa vulgaris). The pathogen grew on all media, but growth rates showed significant differences among media and H. fraxineus isolates. Growth rates were highest on media containing F. excelsior and F. angustifolia, intermediate on media containing O. europaea and P. latifolia and lowest on those containing F. ornus and L. vulgare

Petter Françoise's insight:

See also the EPPO Diagnostic protocol on this pest

http://onlinelibrary.wiley.com/enhanced/doi/10.1111/epp.12061/ ;

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2nd Q-collect project Workshop

2nd Q-collect project Workshop | Diagnostic activities for plant pests | Scoop.it
A Workshop for plant pest collections and for users of biological material -
Rome, 2015-09-08/09
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Read a summary of the meeting and conclusions. 

A white paper on collections was also finalized during this meeting. 

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Detection of Citrus leprosis virus C using specific primers and TaqMan probe in one-step real-time reverse-transcription polymerase chain reaction assays

Detection of Citrus leprosis virus C using specific primers and TaqMan probe in one-step real-time reverse-transcription polymerase chain reaction assays | Diagnostic activities for plant pests | Scoop.it
Citrus leprosis virus C
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LepIntercept: an identification resource for intercepted Lepidoptera larvae

LepIntercept: an identification resource for intercepted Lepidoptera larvae | Diagnostic activities for plant pests | Scoop.it
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A useful ressource!

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A useful ressource!

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Improved morphological key to the species of the xylophilus group of the genus Bursaphelenchus Fuchs, 1937 - Braasch & Schönfeld 2015

Improved morphological key to the species of the xylophilus group of the genus Bursaphelenchus Fuchs, 1937 - Braasch & Schönfeld 2015 | Diagnostic activities for plant pests | Scoop.it
Correct identification of the pine wood nematode Bursaphelenchus xylophilus becomes more difficult, as more related or similar species are described. Spread of the nematode is being monitored worldwide, and in view of the fact that several new Bursaphelenchus species have been described, identification keys should be adjusted accordingly. Based on the identification key of the xylophilus group published by Braasch (2008) and the revised intrageneric grouping of Bursaphelenchus by Braasch et al. (2009), an improved dichotome morphological key including newly described species (B. paraluxuriosae, B. populi, B. macromucronatus, B. firmae, B. koreanus and B. gillanii) is presented. The recognition of the quarantine pest B. xylophilus using morphological characteristics in laboratories of the National Plant Protection Services is facilitated by a simplified key
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EPPO Bulletin - Volume 46, Issue 2 - August 2016 - Wiley Online Library

EPPO Bulletin - Volume 46, Issue 2 - August 2016 - Wiley Online Library | Diagnostic activities for plant pests | Scoop.it
Revised EPPO diagnostic protocols published! they are freely available online
PM 7/41 (3) Meloidogyne chitwoodi and Meloidogyne fallax
PM 7/103 (2) Meloidogyne enterolobii 
PM 7/42 (3) Clavibacter michiganensis subsp. michiganensis 
PM 7/60 (2) Pantoea stewartii subsp. stewartii 
PM 7/77 (2) Documentation and reporting on a diagnosis 
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dPCR EXPERIENCE WORKSHOP Practical workshop on different dPCR platforms 5th – 7th October 2016, National Institute of Biology, Ljubljana, SloveniaEmpty title

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A training course on digital PCR
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A revision of the EPPO Diagnostic Protocol on Xylella fastidiosa sent for EPPO Member Country Consultation 

A revision of the EPPO Diagnostic Protocol on Xylella fastidiosa sent for EPPO Member Country Consultation  | Diagnostic activities for plant pests | Scoop.it
A revised version of the EPPO Diagnostic Protocol on Xylella fastidiosa was prepared by an Expert Working Group and in consultation with experts from Brazil and North America (see section acknowledgements for details).
This revised Standard has been sent for Country Consultation.  According to the EPPO Standard setting procedures comment should only be made through the National Plant Protection Organization official contact point . 
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A revision of the EPPO Diagnostic Protocol on Xylella fastidiosa sent for EPPO Member Country Consultation
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PM 7/079 (2) Grapevine flavescence dorée phytoplasma -  published

PM 7/079 (2) Grapevine flavescence dorée phytoplasma -  published | Diagnostic activities for plant pests | Scoop.it
A revision of the EPPO Diagnostic Protocol on Grapevine flavescence dorée phytoplasma  has been published in the EPPO bulletin. 
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New molecular tests included
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Testa - EPPO Conference on diagnostics for plant pests

Testa - EPPO Conference on diagnostics for plant pests | Diagnostic activities for plant pests | Scoop.it

The EPPO-TESTA Conference was organized in Angers on 2015-11-30/12-02 at the kind invitation of the Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail (ANSES, FR) and of the Groupe d'Etude et de contrôle des Variétés et des Semences (GEVES, FR)

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Read the summary of the meeting and the main conclusions and recommendations

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Assessment of a new qPCR tool for the detection and identification of the root-knot nematode Meloidogyne enterolobii by an international test performance study - Springer

Assessment of a new qPCR tool for the detection and identification of the root-knot nematode Meloidogyne enterolobii by an international test performance study - Springer | Diagnostic activities for plant pests | Scoop.it
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Simultaneous detection of Clavibacter michiganensis subsp. michiganensis, Pepino mosaic virus and Mexican papita viroid by non-radioactive molecular hybridization using a unique polyprobe - Springer

Simultaneous detection of Clavibacter michiganensis subsp. michiganensis, Pepino mosaic virus and Mexican papita viroid by non-radioactive molecular hybridization using a unique polyprobe - Springer | Diagnostic activities for plant pests | Scoop.it

Clavibacter michiganensis subsp. michiganensis, Pepino mosaic virus, and Mexican papita viroidare three economically important pathogens that infect tomato crops. In this work, a polyprobe (poly-3) was developed and evaluated for the simultaneous detection of these pathogens in tomato plants by non-isotopic molecular hybridization. The endpoint detection limit of the poly-3 with C. michiganensis subsp. michiganensis cell cultures was 106 CFU/ml. No differences were found in terms of the sensitivity and specificity when the individual riboprobes were compared to the poly-3 for the detection of the three pathogens. The analysis of 80 tomato field samples by the poly-3 and RT-PCR techniques rendered the same number of positive samples for each pathogen. As far as we know this is the first time that three pathogens with very different life cycle styles (bacteria, virus and viroid) are simultaneously detected in a single assay. The possibility of using this poly-3 technology for the routine diagnosis of field tomato samples is discussed.

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3rd Workshop for Heads of Laboratories Rome, 2015-09-08/11

3rd Workshop for Heads of Laboratories Rome, 2015-09-08/11 | Diagnostic activities for plant pests | Scoop.it
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View the presentations and read the summary and conclusions of the Workshop

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A real-time TaqMan PCR assay to discriminate between pathotype 1 (D1) and non-pathotype 1 (D1) isolates of Synchytrium endobioticum - Springer

A real-time TaqMan PCR assay to discriminate between pathotype 1 (D1) and non-pathotype 1 (D1) isolates of Synchytrium endobioticum - Springer | Diagnostic activities for plant pests | Scoop.it
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The EPPO Diagnostic Protocol on Synchytrium endobioticum is under revision and will be studied at the next meeting of the PAnel on DIagnostics in Mycology in November 2015.

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Development and Validation of LNA-Based Quantitative Real-Time PCR Assays for Detection and Identification of the Root-Knot Nematode Meloidogyne enterolobii in Complex DNA Backgrounds

Development and Validation of LNA-Based Quantitative Real-Time PCR Assays for Detection and Identification of the Root-Knot Nematode Meloidogyne enterolobii in Complex DNA Backgrounds | Diagnostic activities for plant pests | Scoop.it
Phytopathology, Ahead of Print.
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An EPPO diagnostic protocol on Meloidogyne enterolobii PM 7/103 is under revision.  

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Two revised EPPO diagnostic protocols published in the EPPO Bulletin

Two revised EPPO diagnostic protocols published in the EPPO Bulletin | Diagnostic activities for plant pests | Scoop.it

PM 7/46 (3) Lecanosticta acicola (formerly Mycosphaerella dearnessii),Dothistroma septosporum

PM 7/048 (3) Plenodomus tracheiphilus 

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Two revised EPPO diagnostic protocols published and available freely online.

 

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9th Meeting of the Panel on Diagnostics in Nematology

9th Meeting of the Panel on Diagnostics in Nematology | Diagnostic activities for plant pests | Scoop.it

Read a short report of the 9th Meeting of the Panel on Diagnostics in Nematology 

Petter Françoise's insight:

Keep updated with the EPPO programme of preparation of diagnostic protocols in nematology

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3rd Meeting of the Panel on Diagnostics in Entomology

3rd Meeting of the Panel on Diagnostics in Entomology | Diagnostic activities for plant pests | Scoop.it

Short report of the 3rd Meeting of the Panel on Diagnostics in Entomology. 

Petter Françoise's insight:

Keep updated with the EPPO programme of preparation of diagnostic protocols in entomology

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