Another helpful bit of Mycoplasma information – everyone doing cell culture should know this stuff!
Let’s say you have a sample where you need a high degree of confidence that it is Mycoplasma free. For example, you may have treated a culture for Mycoplasma and you want to see if there are viable organisms still left before you proceed to an important assay. What do you do?
Enrichment is a useful resource to keep in mind when you are dealing with an important sample. In this context, enrichment (also known as co-cultivation) refers to taking medium from your culture and inoculating it onto a cell line that is known to support the rapid growth of a broad number of Mycoplasma species. You then test the enriched cell line to see if Mycoplasma can be detected using your preferred test method. This approach increases the sensitivity of many of the test methods out there, allowing you to get more informative results.
If you are tempted to give enrichment a try, then the link above is well worth visiting. This paper from scientists at the Food and Drug Administration looks in detail at enrichment – what cell lines are best to use, what species can be detected as a result.
Just something to keep in mind – you never know when it might be useful....
Via Amanda Capes-Davis