Cloning (Aspect 2) and Stem Cell Therapy (Aspect 3)
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Cloning Fact Sheet

Cloning Fact Sheet | Cloning (Aspect 2) and Stem Cell Therapy (Aspect 3) | Scoop.it
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Tim Gongaware's comment, March 4, 2014 12:43 PM
4. To perform reproductive cloning, first a mature somatic cell must be removed from the animal you wish to clone. Then the DNA from the mature somatic cell is implanted into an egg cell from another donor, this egg cell has its nucleus removed. There are two ways to implant the somatic cell DNA into the egg cell, one way is by taking somatic cell DNA and directly putting it into the egg cell and the other way is by using electricity to make the two cells as one. Next the egg begins the process to becoming an embryo in a test-tube, following that, is implanted into a mature female animal to act as the surrogate mother. The embryo then matures as a normal one would and is born, being a clone of the donor of the somatic cell.
Tim Gongaware's comment, March 4, 2014 12:52 PM
<br>5. To copy a gene in gene cloning, first a gene is inserted into the DNA of a vector, which carries the newly added gene. Some vectors are bacteria, yeast cells, plasmids, viruses, among others. Then this vector is then reproduced asexually, producing with the desired gene carried.<br>
Tim Gongaware's comment, March 5, 2014 11:36 AM
6. Therapeutic cloning creates cloned embryos to harvest their stem cell. Much like other cloning, this can cause the stem cell to have the donor’s DNA. The use of these stem cells is to treat genetic diseases and other such conditions.
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Learn.Genetics.utah.edu

Learn.Genetics.utah.edu | Cloning (Aspect 2) and Stem Cell Therapy (Aspect 3) | Scoop.it
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Tim Gongaware's comment, March 28, 2014 12:43 PM
8. (Aspect 3) iPS cells, or induced pluripotent stem cells, are man-made stem cells utilizing a patient’s personal cells. These cells can be made from any cell, like a fat or skin cell. The cells seem to be able to differentiate into any type of cell, but more research is needed to confirm this. Similar to STAP cells, they will not be rejected and can be used to treat many types of disease. These cells are cheaper to make than embryonic stem cells, but are created by modifying the genes of a cell, possibly causing mutations, among other things.
Tim Gongaware's comment, March 28, 2014 12:50 PM
9. (Aspect 3) Another method of creating embryonic stem cells is by therapeutic cloning. Unlike regularly made embryonic stem cells, these stem cells are made utilizing the patient’s DNA, and as such, is not rejectable. As usual, these cells can become any other type of cell. Creating the actual cells involves isolating cells from the patient, removing the nucleus from an egg cell, then taking the nucleuses from the patient’s donated cell into the egg cell. Then the egg cell transforms the nucleus DNA to match it. Finally the cell begins to divide and the stem cells are harvested during the blastocyst stage.
Tim Gongaware's comment, March 31, 2014 12:24 AM
10. (Aspect 3) One example of using bone marrow stem cells is to cure leukemia, or cancer of white blood cells. Leukocytes come from somatic stem cells and once they have developed and matured, they are released into the blood to counteract diseases and such in the human body. The cancer appears specifically when these leukocytes behave as a cancer and reproduce uncontrollably. These cancer cells then no longer behave as intended, and disrupt other parts of the body. One way to eliminate this is by chemotherapy, or bone marrow transplant. First the leukocytes that are cancerous are destroyed by chemotherapy and radiation, and then the donated bone marrow stem cells are injected into the patient’s bloodstream. These stem cells will finally then differentiate into new leukocytes for the patient, curing the disease.