The separation between biological and technical variation without extensive use of technical replicates is often challenging, particularly in the context of different forms of protein and peptide modifications. Biosampling procedures in the research laboratory are easier to conduct within a shorter time frame and under controlled conditions as compared to clinical sampling with the later often having issues of reproducibility. But is the research laboratory biosampling really less variable? Biosampling introduces within minutes rapid tissue specific changes in the cellular microenvironment; inducing a range of different pathways associated with cell survival. Biosampling involves hypoxia and hypothermia which are circumstances for which there are evolutionary conserved defense strategies in range of species and also are relevant for a range of biomedical conditions. It remains unclear to what extent such adaptive processes are reflected in different biosampling procedures or how important they are for the definition of sample quality. Lately, an increasing number of comparative studies on different biosampling approaches, post-mortem effects and pre-sampling biological state have investigated such immediate early biosampling effects. Commonalities between biosampling effects and a range of ischemia/reperfusion and hypometabolism/anoxia associated biological phenomena indicate that even small variations in post-sampling time intervals are likely to introduce a set of non-random and tissue specific effects of experimental importance (both in vivo and in vitro). This review integrates the information provided by these comparative studies and discusses how an adaptive biological perspective in biosampling procedures may be relevant for sample quality issues.
A European-led consortium of researchers and biotech firms are working together to develop and manufacture individualized vaccines based on the genomic, proteomic, and peptidomic characteristics of a particular glioblastoma patient's tumor and...
Quantitative Proteomics in Biomarker Discovery. The ultimate aim of biomarker discovery is to develop a simple differential test to be used as a clinical evaluation tool. This requires a lengthy and difficult process which involves candidate ...
Our overall goal was to develop a workflow leading from standardized preparation of serum samples, through data management facilitating the design of MRM quantification, to the validation in a clinically applicable assay.
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