Compared to JC-1, JC-10 has much better water solubility. JC-10 is capable of selectively entering into mitochondria, and reversibly changes its color from green to orange as membrane potentials increase. When excited at 490 nm, the color of JC-10 changes reversibly from green to orange as the mitochondrial membrane becomes more polarized. The green emission can be analyzed in fluorescence channel 1 (FL1) and orange emission in channel 2 (FL2).
Cell Meter™ JC-10 Mitochondrial Membrane Potential Assay Kit (Cat# 22800) enables researchers to run JC-10 assay in the format of microplate reader, and it provides the most robust assay method for monitoring mitochondria membrane potential changes. The kit is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye. In normal cells, JC-10 concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. However, in apoptotic and necrotic cells, JC-10 diffuses out of mitochondria. It changes to monomeric form and stains cells in green fluorescence. The kit can be used to screen both apoptosis activators and inhibitors. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.
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