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Everything You Always Wanted to Know About Genome Assembly* (*But Were Afraid to Ask)
De novo genome sequencing of previously uncharacterized microorganisms has the potential to open up new frontiers in microbial genomics by providing insight into both functional capabilities and biodiversity.
a lot ... A LOT ... on genomic tools comparisons
fine review and test for available techniques
Paving roads through data mountains, consortia are developing workflows and tools for widespread use.
Assembly algorithms have been extensively benchmarked using simulated data so that results can be compared
Find N50 too simplistic as an assembly quality metric? The Pachter lab present a more sophisticated approach.
this is biota case - where you never have a general truth genome
Top ten lists have taken a bit of a battering in recent times, and so I kind of apologise for adding another one, but I’m not really that sorry. Some of you will be aware that I manage a geno...
Pay attention particularly on point 4: #Bioinformatics ;)
one more to the genomic moneybox of assembley algorithms
Improved microbial BLAST has new database options including Representative genomes plus links to Entrez Genome
I am a fan of the Pacific Biosciences PacBio RS sequencing instrument, as some of the readers of this blog maybe already are aware of. We use data from this instrument in our work, and the Norwegia...
The CBiB - Bordeaux Bioinformatics Center has been approved by Lloyd's Register Quality Assurance to the following Quality Management System Standards : ISO 9001:2008
A potential user (‘customer’) of our sequencing platform asked how to generate reference genomes for his 4 bacterial strains. His question inspired me to write this post. The suggestion...