Cell - Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression | Cas9 | Scoop.it

Highlights:

 

-->Inactive CRISPR associated 9 protein (dCas9) is repurposed for genome engineering

 

-->dCas9 and a complementary short guide RNA can target specific genomic sites

 

-->CRISPR interference (CRISPRi) can regulate multiple genes without off-target effects

 

-->CRISPRi is compact and can be ported to bacterial and mammalian cells


Via Amir Taheri Ghahfarokhi