Biology, Biotechnology, Medical research
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sRNA-seq analysis of human embryonic stem cells and definitive endoderm reveal differentially expressed microRNAs and novel isomiRs with distinct targets - Hinton - STEM CELLS - Wiley Online Library

sRNA-seq analysis of human embryonic stem cells and definitive endoderm reveal differentially expressed microRNAs and novel isomiRs with distinct targets - Hinton - STEM CELLS - Wiley Online Library | Biology, Biotechnology, Medical research | Scoop.it
Philippe Fixe's insight:

A practical example of the benefit of RNA-Seq in cell differentiation (here from HESC to Definitive Endoderm).

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Microfluidics and Single Cells

Microfluidics and Single Cells | Biology, Biotechnology, Medical research | Scoop.it
Growing evidence suggests that biochemical assays don’t capture the complexity of cell cultures. Now, researchers are turning to microfluidics to assay one cell at a time. Learn more...

Via Jean-Francois TETU
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New cell line should accelerate embryonic stem cell research | UW HSNewsBeat

New cell line should accelerate embryonic stem cell research | UW HSNewsBeat | Biology, Biotechnology, Medical research | Scoop.it
Philippe Fixe's insight:

A new in vitro Stem Cellular model (naïve cell) allowing a deeper understanding of the development of human cells. This EIf1 cell line has been generated without adding a set of artificial genes in the "reverse toggled" recipient cells. 

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5 key criteria for selecting the most appropriate ELISA kit to detect Human Cardiotrophin-1

5 key criteria for selecting the most appropriate ELISA kit to detect Human Cardiotrophin-1 | Biology, Biotechnology, Medical research | Scoop.it
Cardiotrophin-1 (CT-1) is a cardiac hypertrophic factor with cardiac myocyte protective properties. CT-1 belongs to the interleukin-6 cytokine family and acts through LIF receptor ß/glycoprotein 13...
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Brain Implants: The Laser Eye Surgery of the Future?

Brain Implants: The Laser Eye Surgery of the Future? | Biology, Biotechnology, Medical research | Scoop.it

Brain implants today are where laser eye surgery was several decades ago, fraught with risk, applicable only to a narrowly defined set of patients – but a sign of things to come. 


Via Szabolcs Kósa
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aanve's curator insight, March 20, 2014 3:34 AM
www.aanve.com
zwilenkosi's curator insight, March 25, 2014 2:41 PM

new brain implants that could revive your memory and makes you to learn things fast.

 

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Sanofi, UCB team up in $138M deal aimed at tackling biologic blockbusters

Sanofi, UCB team up in $138M deal aimed at tackling biologic blockbusters | Biology, Biotechnology, Medical research | Scoop.it
Sanofi is joining forces with UCB to study new small-molecule approaches to treating inflammatory conditions like arthritis, looking for an easier form of administration to some of the blockbuster injected biologics that dominate the field.
Philippe Fixe's insight:

Sanofi collaborates with UCB to study new small-molecule approaches to treating inflammatory conditions.

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Brain cancer stem cells from human brain cancer tissue | 2013 Photomicrography Competition | Nikon Small World

Brain cancer stem cells from human brain cancer tissue | 2013 Photomicrography Competition | Nikon Small World | Biology, Biotechnology, Medical research | Scoop.it
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GCNF-dependent activation of cyclin D1 expression via repression of Mir302a during ES cell differentiation - Wang - STEM CELLS - Wiley Online Library

GCNF-dependent activation of cyclin D1 expression via repression of Mir302a during ES cell differentiation - Wang - STEM CELLS - Wiley Online Library | Biology, Biotechnology, Medical research | Scoop.it
Philippe Fixe's insight:

Physiological development:
The Cyclin D1 expression is activated during the development of ES cells via inhibition of mir302a through the Germ Cell Nuclear Factor (GCNF) pathway.

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EpiCypher, Inc. epigenetics research tools

EpiCypher, Inc. epigenetics research tools | Biology, Biotechnology, Medical research | Scoop.it
Epicypher from tebu-bio: your partner in Epigenetics and Chromatine biology research.
Philippe Fixe's insight:

New technologies for studying Epigenetics events & Chromatin biology in Stem cell research: from recombinant nucleosomes to enzymes to Histone peptide arrays.

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New ELISA to quantify IFNAR2 | tebu-bio

New ELISA to quantify IFNAR2 | tebu-bio | Biology, Biotechnology, Medical research | Scoop.it
VeriKine™ Human Interferon Alpha/Beta Receptor 2 ELISA Kit
Philippe Fixe's insight:

This new Verikine Human Interferon alpha/beta receptor ELISA (from PBL Assay Science) is an ideal tool for biomarker studies in oncology and autoimmune diseases and, a robust companion for cell signaling studies involving IFN pathways.

*Assay range: 3,1 - 200 pg/ml

*< 10% intra- & inter-CVs

*Compatible with serum, plasma, cell culture supernatant & cell *lysates

 

This new tool allows researchers to reliably measure human IFNAR2 and go further in the understanding of IFN biology!

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Proteomic Analysis of C2C12 Myoblast and Myotube Exosome-Like Vesicles: A New Paradigm for Myoblast-Myotube Cross Talk?

Proteomic Analysis of C2C12 Myoblast and Myotube Exosome-Like Vesicles: A New Paradigm for Myoblast-Myotube Cross Talk? | Biology, Biotechnology, Medical research | Scoop.it
PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
Philippe Fixe's insight:

A new publication with research antibodies provided by tebu-bio:

 

Forterre et al.: Roles of muscle-derived exosomes in intercellular communications by analysing the protein contents of the exosome-like vesicles released from murine myoblasts with a proteomic approach mixing electron microscopy, western-blot and bioinformatic analyses.



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The Polycomb Protein Ezh2 Impacts on iPS Cell Generation | Abstract

The Polycomb Protein Ezh2 Impacts on iPS Cell Generation | Abstract | Biology, Biotechnology, Medical research | Scoop.it
Philippe Fixe's insight:

Histone-lysine N-methyltransferase (EZH2), a core subunit of Polycomb repressive complex 2 (PRC2), is critical for efficient iPS cell generation. It acts during reprogramming at least in part through repressing the Ink4a/Arf locus. In addition, EZH2 is involved in maintaining the reprogrammed iPS cell state.

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Establishing meaningful SYBR Green-based qPCR assays | Technical tips from tebu-bio experts

Establishing meaningful SYBR Green-based qPCR assays | Technical tips from tebu-bio experts | Biology, Biotechnology, Medical research | Scoop.it
An overview and technical tips for optimal SYBR Green qPCR with Standard Curve Protocol
Philippe Fixe's insight:

A very complete and optimized protocol compatible with the StepOnePlus™ Real-Time PCR System (Applied Biosystems®).

This detailled procedure is meant to help users generate meaningful and reliable qPCR results.

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Bioactive small molecules make exogenous reprogamming genes dispensable

Bioactive small molecules make exogenous reprogamming genes dispensable | Biology, Biotechnology, Medical research | Scoop.it
Cell reprogramming of Mouse and Human somatic cells to induced Pluripotent Stem Cells (iPSC) have opened outstanding new opportunities for biomedical research. iPSC are now becoming unique cellular...
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PV-10's Assessment For Drug-Drug Interaction Potential

PV-10's Assessment For Drug-Drug Interaction Potential | Biology, Biotechnology, Medical research | Scoop.it
The published research indicated that the risk of PV-10 causing clinically relevant drug-drug interactions is likely minimal. PV-10, a 10% solution of rose bengal that is currently under clinical i...
Philippe Fixe's insight:

A nice example of collaborative wok for Drug-Drug Interaction studies

#ADME 

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Rescooped by Philippe Fixe from Cancer - Advances, Knowledge, Integrative & Holistic Treatments
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A Gene Family That Suppresses Prostate Cancer

A Gene Family That Suppresses Prostate Cancer | Biology, Biotechnology, Medical research | Scoop.it
Cornell University researchers report they have discovered direct genetic evidence that a family of genes, called MicroRNA-34 (miR-34), are bona fide tumor suppressors.

Via Graham Player Ph.D.
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Graham Player Ph.D.'s curator insight, March 14, 2014 9:00 AM

It is well accepted that mutations of the p53 gene have been implicated in half of all cancers. Research in mice has revealed how interplay between genes p53 and miR-34 (MicroRNA-34) jointly inhibits another cancer-causing gene called MET. In absence of p53 and miR-34, MET overexpresses a receptor protein and promotes unregulated cell growth and metastasis.

In mice that had both miR-34 and p53 silenced concurrently, cancerous lesions formed in a proximal part of the prostrate ducts, in a compartment known to contain prostate stem cells. The early lesions that developed when p53 was silenced alone occurred in a distal part of the ducts, away from the compartment where the stem cell pool is located. This suggested there was another mechanism involved when p53 and miR-34 were jointly silenced.

These findings suggest that drug therapies that target and suppress MET could be especially successful in cancers where both p53 and miR-34 are deficient. This is the first time this mechanism has been proven in a mouse model, said Alexander Nikitin, a professor of pathology in Cornell's Department of Biomedical Sciences. "These results indicated that together miR-34 and p53 regulate the prostate stem cell compartments," said Nikitin.

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Stem cell-derivative Motor Neurons / Muscle fiber co-culture functional bio-assay

Stem cell-derivative Motor Neurons / Muscle fiber co-culture functional bio-assay | Biology, Biotechnology, Medical research | Scoop.it
Chipman et al. (Dalhousie University, Halifax, Nova Scotia, Canada) have developed a new cell-based system "mimicking" in vitro Mature Neuromuscular Junction (NMJs). (1) NMJs play an important role...
Philippe Fixe's insight:

Phenotype cell-based in vitro assay for Motor Neuron Diseases

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Human Liver MyoFibroblasts - HLMF | tebu-bio

Human Liver MyoFibroblasts - HLMF | tebu-bio | Biology, Biotechnology, Medical research | Scoop.it
Human Liver MyoFibroblasts (HLMFs) represent a heterogenous population of alpha-smooth muscle actin (α-SMA)-positive pro-fibrogenic cells, mainly found in chronically injured livers (i.e., fibrotic and or cirrhotic).
Philippe Fixe's insight:

Human primary cell sourcing for R&D research

tebu-bio experts have found a source for qualified Normal and Cirrhotic Human Liver Myofibroblasts (HLMF). These rare primary cells are ideal in vitro cellular models for toxicology studies and liver-diseases studies.

 

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Photography competition

Photography competition | Biology, Biotechnology, Medical research | Scoop.it
Every year the Society of Biology runs an amateur biology themed photography competition. The 2014 competition will be launched in spring 2014. (Our annual photo competition is now open for entries!
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CellNEWS: Converting Adult Human Cells to Hair-follicle Generating Stem Cells

CellNEWS: Converting Adult Human Cells to Hair-follicle Generating Stem Cells | Biology, Biotechnology, Medical research | Scoop.it
One approach to reversing hair loss is to use stem cells to regenerate the missing or dying hair follicles. But it hasn't been possible to generate sufficient number of hair-follicle-generating stem cells – until now.
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Stabilise & visualise acti filaments in vitro! | tebu-bio

Stabilise & visualise acti filaments in vitro! | tebu-bio | Biology, Biotechnology, Medical research | Scoop.it
Develop unique and exceptionally bright & stable F-actin probes economically.
Philippe Fixe's insight:

A nice stain from Cytoskeleton Inc. to visualize actin filaments.

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CellNEWS: Scientists Make Living Brain Cells from Alzheimer's Patients Biobanked Brain Tissue

CellNEWS: Scientists Make Living Brain Cells from Alzheimer's Patients Biobanked Brain Tissue | Biology, Biotechnology, Medical research | Scoop.it
Scientists at The New York Stem Cell Foundation (NYSCF) for the first time generated induced pluripotent stem (iPS) cells lines from non-cryoprotected brain tissue of patients with Alzheimer's disease.
Philippe Fixe's insight:

First time generation of iPS cells lines from non-cryoprotected brain tissue of patients with Alzheimer's disease: a new tool for AD drug development and diagnosis?

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JTM | Full text | Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors

JTM | Full text | Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors | Biology, Biotechnology, Medical research | Scoop.it
CD34+ progenitor cells comprise both hematopoietic and endothelial progenitor cells.
Philippe Fixe's insight:

By producing pro-angiogenic factors, pancreatic carcinoma cells attract CD34+ circulating cells to the tumor site and, influence their differentiation into endothelial cells to become a component of the tumor vasculature.

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Tips and tricks to design and test qPCR primers - advanced procedure | tebu-bio

Tips and tricks to design and test qPCR primers - advanced procedure | tebu-bio | Biology, Biotechnology, Medical research | Scoop.it
Design and test your own qPCR primers (for the truly motivated scientist!)
Philippe Fixe's insight:

Based on their experience in PCR, tebu-bio experts have edited this document summarizing a complete procedure with tips and tricks for the optimal design of qPCR primers. Enjoy!

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