Science 23 November 2012:
Vol. 338 no. 6110 pp. 1088-1093
Decoding Human Cytomegalovirus
Noam Stern-Ginossar1, Ben Weisburd1, Annette Michalski2,*, Vu Thuy Khanh Le3, Marco Y. Hein2, Sheng-Xiong Huang4, Ming Ma4, Ben Shen4,5,6, Shu-Bing Qian7, Hartmut Hengel3, Matthias Mann2, Nicholas T. Ingolia1,†, Jonathan S. Weissman1,*
The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity.
Puedes leer el artículo completo en: http://www.sciencemag.org/content/338/6110/1088.full
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