Evan J Olson, Jeffrey J Tabor "Synthetic biology is improving our understanding of and ability to control living organisms. To date, most progress has been made by engineering gene expression. However, computational and genetically encoded tools that allow protein activity and protein–protein interactions to be controlled on their natural time and length scales are emerging. These technologies provide a basis for the construction of post-translational circuits, which are capable of fast, robust and highly spatially resolved signal processing. When combined with their transcriptional and translational counterparts, synthetic post-translational circuits will allow better analysis and control of otherwise intractable biological processes such as cellular differentiation and the growth of tissues.
Highlights ► Phytochromes and LOV domains are being used for spatiotemporal control of protein activity in cells. ► Computational redesign of CDC42/ISTN interface generates orthogonal signaling pathway. ► Computation-guided design of 180 pmKd PPI paves way for the design of modular protein networks. ► Stem cell differentiation can be controlled with synthetic transcriptional and post-translational networks."
"...in their constant battles with competitors and the host immune system, (opportunistic) microbial pathogens have developed sophisticated cell–cell communication systems termed quorum sensing (QS) that allow exchange of critical information. In return, competing microbes, as well as the host immune system, have developed means to intercept and decode these messages..
To illustrate the clinical importance of this microbial spy game, we will focus on the biological activity of a single bacterial QS molecule on surrounding microbes and the host immune system and its diverse “meaning” to different receivers."
Church lab publications - especially interesting the list of submitted/revision "255. Vigneault F, Laserson U, Simen BB, Lieberman-Aiden E, Egholm M, Church GM (2012) Tracking the dynamics of a human antibody repertoire. (in revision)
254. Jewett MC, Church GM (2012) In vitro integration of ribosomal RNA synthesis, ribosome self-assembly and protein synthesis. Nature (in revision).
253. Carr PA, Wang HH, Sterling B, Isaacs FJ, Xu G, Church GM, Jacobson JM (2012) Enhanced Multiplex Genome Engineering through Oligonucleotide Co-selection (in revision)
252. Juhas M, Eberl L, Church GM (2012) Essential genes as antimicrobial targets and cornerstones of synthetic biology (submitted)
251. Boyle PM, Burrill DR, Inniss MC, Agapakis CM, Deardon A, DeWerd JG, Gedeon MA, Quinn JY, Paull ML, Raman AM, Theilmann MR, Wang L, Winn JC, Medvedik O, Schellenberg K, Haynes KA, Viel A, Brenner TJ, Mathews S, Church GM, Shah JV, Silver PA (2012) Personalized Genetic Engineering of Plants. (submitted)
250. Ball MP*, Thakuria JV*, Zaranek AW*, Clegg T, Rosenbaum AM, Wu X, Angrist M, Bhak J, Bobe J, Callow M, Cano C, Chou MF, Chung WK, Douglas SM, Estep P, Gore A, Hulick P, Labarga A, Lee J, Lunshof J, Kim BC, Kim JI, Li Z, Murray MF, Nilsen GB, Peters B, Raman AM, Reinhoff HY, Robasky K, Wheeler M, Vandewege W, Vorhaus D, Yang JL, Yang L, Aach J, Ashley EA, Drmanac R, Kim SJ, Li JB, Peshkin L, Seidman CE, Seo JS, Zhang K, Rehm HL, Church GM (2012) A Public Resource Facilitating Clinical Use of Genomes (submitted as PNAS inaugural article)
249. Peters BA, Kermani BG, Sparks AB, Alferov O, Hong P, Alexeev A, Jiang Y, Dahl F, Tang YT, Hass J, Robasky K, Zaranek AW, Lee JH, Ball MP, Peterson JE, Perazich H, Yeung G, Liu J, Chen L, Pothuraju K, Konvicka K, Tsoupko-Sitnikov M, Pant KP, Ebert J, Nilsen G, Baccash J, Halpern AL, Church GM, Drmanac R (2012) Clinically accurate genome sequencing and haplotyping from 10-20 human cells using massively parallel short reads on long DNA fragments (submitted to Nature)
248. Wang HH, Kim H, Cong L, Jeong J, Bang D, Church GM (2012) Genome-scale Promoter Engineering by Co-Selection MAGE (Nature Methods in press)
247. Wang HH, Huang PY, Xu G, Haas W, Marblestone A, Li J, Gygi S, Forster A, Jewett MC, Church GM (2012) Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multi-enzyme catalysis. (ACS Synthetic Biology, in press)"
"Scientists are combining biology and engineering to change the world.
With a box of Lego, you can create a whole range of different structures. Snap together pieces of various colours, shapes and sizes to create a multitude of structures — a house, a boat, a tower — with different functions. In the world of biology, a growing group of scientists is thinking about parts of cells in much the same way.
Engineers are using genes and proteins as building blocks to create new kinds of cell and new functions for cells. If scientists can build genes from scratch, they can create organisms with new traits. They can create bacteria that can clean up oil spills, rice with genes that keep the plant infection-free, or cells that can churn out new materials. Synthetic biology, the field that revolves around figuring out how to combine genes in new and interesting ways, requires an understanding of biology, creative engineering skills and computing expertise. It is pulling together scientists with different capabilities to solve problems....."
In a very insightful article, published in this month in the journal Nature, James Collins, a bioengineer professor at Boston University explains what synthetic biology is, and gives an overview of its potential opportunities and ...
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