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Updated information on a "new disease" in rainbow trout - Norwegian Veterinary Institute

Updated information on a "new disease" in rainbow trout - Norwegian Veterinary Institute | Aquaculture Directory | Scoop.it

In late August 2013 the Norwegian Veterinary Institute (NVI) received the first case of diseased rainbow trout from a hatchery. The signs of disease were unusual for fish at this age. The second and third cases were submitted to the NVI during October and primo November. The most recent hatchery affected by this disease was recorded in January 2014. Diseased fish were sized from 30 – 100 g. One hatchery reported high mortality in some pens. The other affected sites reported moderate mortalities.  


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John Bostock's curator insight, July 3, 9:33 AM

Information on an emerging disease issue in Norway

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Cutthroat trout virus as a surrogate in vitro infection model for testing inhibitors of hepatitis E virus replication

Cutthroat trout virus as a surrogate in vitro infection model for testing inhibitors of hepatitis E virus replication | Aquaculture Directory | Scoop.it

Hepatitis E virus (HEV) is one of the most important causes of acute hepatitis worldwide. Although most infections are self-limiting, mortality is particularly high in pregnant women. Chronic infections can occur in transplant and other immune-compromised patients. Successful treatment of chronic hepatitis E has been reported with ribavirin and pegylated interferon-alpha, however severe side effects were observed. We employed the cutthroat trout virus (CTV), a non-pathogenic fish virus with remarkable similarities to HEV, as a potential surrogate for HEV and established an antiviral assay against this virus using the Chinook salmon embryo (CHSE-214) cell line. Ribavirin and the respective trout interferon were found to efficiently inhibit CTV replication. Other known broad-spectrum inhibitors of RNA virus replication such as the nucleoside analog 2′-C-methylcytidine resulted only in a moderate antiviral activity. In its natural fish host, CTV levels largely fluctuate during the reproductive cycle with the virus detected mainly during spawning. We wondered whether this aspect of CTV infection may serve as a surrogate model for the peculiar pathogenesis of HEV in pregnant women. To that end the effect of three sex steroids on in vitro CTV replication was evaluated. Whereas progesterone resulted in marked inhibition of virus replication, testosterone and 17β-estradiol stimulated viral growth. Our data thus indicate that CTV may serve as a surrogate model for HEV, both for antiviral experiments and studies on the replication biology of theHepeviridae.


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John Bostock's curator insight, September 24, 2013 1:20 PM

This could open a few new doors for fish virologists and research facilities!

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Trout in the Classroom gives students hands-on experience

Trout in the Classroom gives students hands-on experience | Aquaculture Directory | Scoop.it

Trout in the Classroom projects got their start in this area, quite appropriately, because of fish.  The program is now rippling out, so hundreds of middle and high school students each year learn more about fish, streams, stream ecology and how to protect the water.


Via Perendale Publishers (Tuti Tan), John Bostock
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John Bostock's curator insight, February 8, 2:06 PM

Good example of introducing fish culture to school students as part of a wider learning agenda.

Serge @ FARNET.'s curator insight, February 10, 9:12 AM

Great idea to help fish migrate from the school's menu to the school's program

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Novel insights into the peritoneal inflammation of rainbow trout (Oncorhynchus mykiss)

Novel insights into the peritoneal inflammation of rainbow trout (Oncorhynchus mykiss) | Aquaculture Directory | Scoop.it

The peritoneal cavity has been extensively used as a laboratory model of inflammation in many species, including the teleost fish. Although, the peritoneal cavity of rainbow trout (Oncorhynchus mykiss) was previously shown to contain a resident population of leukocytes, closer information about their exact composition and their functional response to pathogens is still missing. In the presented work, flow cytometric analysis using monoclonal antibodies was performed to characterize this cell population and evaluate its traffic during the first 72 h after antigenic stimulation and infection with Aeromonas salmonicida. Obtained results indicate that the unstimulated peritoneal cavity represents rather a lymphoid niche, dominated by the IgM+ B cells. Expectedly, the composition changed rapidly after stimulation, which resulted in two complete changes of dominant cell type within first 72 h post injection. While the first stage of inflammation was dominated by myeloid cells, lymphocytes predominated at the later time points, with IgM+B cells representing more than two thirds of all cells. Later, the infection experiment elucidated the peritoneal infection and identified the key differences to the antigenic stimulation. Additionally, the data indicate that the resolution of the inflammation depends more on the bacterial clearance by myeloid cells than on regulation by lymphocytes. Taken together, obtained results represent the first complete description of the immune reaction protecting the peritoneal cavity of the fish and shed some light on the conservation of these processes during the evolution.


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