Takele Abayneh Tefera's doctoral research project has uncovered a genetic divergence between the fish pathogen Edwardsiella tarda and Edwardsiella tarda type strain. He has also identified phenotypic markers that distinguish one from the other. The fish pathogenic strain is now classified as a separate species: Edwardsiella piscicida.
Edwardsiella tarda is a bacterium that can infect a number of animal species and also humans. Edwardsiellosis is one of the most serious systemic bacterial diseases in fish, resulting in substantial losses in the fish farming industry all over the world.
Takele Abayneh Tefera has developed effective molecular tools for the identification and characterization of different strains of Edwardsiella. He developed a new TaqMan real-time and conventional PCR analysis for this purpose and then evaluated it in relation to the Loop Mediated Isothermal Amplification (LAMP) analysis. For the first time, he also used the Multi-locus Sequence Analysis (MLSA) for the typing and characterization of E. tarda, isolated from different sources. This is a useful tool for detecting sources of infection and for understanding the epidemiological relationship between isolates from the environment, fish, livestock and humans.
Via John Bostock