A new method for the detection of AHPND bacteria AP4 has been published. The advantage of the AP4 method over the previously published AP3 method is that the it has 100 times higher sensitivity. Because of its higher sensitivity, the bacterial culture enrichment step needed when using the AP3 with low levels of AHPND bacteria may be omitted. However, the AP4 method should not be considered as a replacement for the AP3 method but simply as an alternative choice for the users to choose should they need a more sensitive detection method.
The AP4 PCR method was developed entirely by Thai scientists working in Thailand at Centex Shrimp,the Shrimp-virus interaction laboratory, BIOTEC and Aquatic Animal Health Research Center and Charoen Pokphand Co. Ltd. It was also supported entirely by research funding from Thailand.
Via John Bostock