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blueAmp: Simple and rapid detection test for streptococcosis in Nile tilapia

blueAmp: Simple and rapid detection test for streptococcosis in Nile tilapia | Aquaculture Directory | Scoop.it

Streptococcosis is a major bacterial infection in many cultured fish species, damaging the production of intensive aquaculture species, particularly Nile tilapia. The infection is caused by Streptococcus agalactiae and Strep. Iniae. 

“blueAmp” has been developed as a simple and rapid assay to detect Strep. agalactiae and Strep. Iniae.  The assay is based on DNA amplification by loop-mediated isothermal amplification (LAMP) and a detection of DNA products by colorimetric change of hydroxynaphthol blue dye. The result can be observed by a color change through the naked eyes.  This method takes approximately an hour, which is significantly less than the time required by a standard test which can take upto 6 days.

“blueAmp” is useful for screening broodstock and fry before stocking and for monitoring fish health in grow-out pond.This technology is developed by Bioengineering and Sensing Technology Laboratory, and is being validated with industry before it becomes available for licensing.


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Ram C. Bhujel's curator insight, June 3, 6:04 PM
Globally, about 70 million tons of seafood is produced from farming annually. More and more people are consuming seafood due its health benefits. Additional 160 million tons will be needed by 2050. After “Green and White Revolutions”, ‘Blue Revolution” is the need of the present which is possible only when thousands of new generation leaders are produced, who ultimately will train millions of other people learn how to breed and grow fish or aquatic animals sustainably applying scientific principles and adopting as profitable businesses. As a result, every country should be able to take advantage of the sector like that by Bangladesh, China, India, Thailand, Vietnam and many others. From seafood export, annually China earns nearly US$20 billion, while India, Thailand and Vietnam each receives US$6-7 billion per year. 

Aquaculture is on the spotlight nowadays. It has attracted so much public attention as well as private investment because it has emerged as one of the most profitable and competitive businesses. So far, we have trained thousands of entrepreneurs from all over the world. Some of them have started their own businesses, while others manage large corporate or government farms profitably. 

Now we plan to start a 10-month, Professional Master's degree program in Aquaculture to offer up-to-date knowledge in aquaculture and help develop skills on proven innovative technologies to interested investors, entrepreneurs, managers and other professionals so that their dreams come true. The followings are the proposed courses: --- 
1. Aqua Business Management - Principles and Practices 
2. Global Aquaculture Business - Managerial Perspective 
3. Business Communication 
4. Environmental Management & Aquaculture Sustainability 
5. Marketing Management 
6. Aquaculture Certification and Quality Control 
7. Aquaculture Project Selection & Management 
8. Integrated Aquaculture 
9. Entrepreneurship and Business Plan Development 
10. Aqua Seed Business 
11. Managing Aquaculture Technology 
12. Seafood Supply Chain Management 
13. Aquaculture Economics and Managerial Accounting 
14. Corporate Social Responsibility and Ethics 
15. Post Harvest Management and Food Safety 
16. Decision Making and Negotiations 
17. Seafood Trade and Business Law 
18. Statistics for Managers 
19. Aqua Feed Business 
20. Fish Health Management 
Case studies: Shrimp/prawn farms, Tilapia Farms, Catfish/Pangasius Farms, Seafood markets, Processing companies, Feed companies, Hatcheries, and so on. 

More details will be available later. First, we would like to have your your comments and suggestions. Please write here comments or send email to: Director@aarm-asialink.info 
Thank you very much.
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Diseases of Aquatic Organisms » Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. I. Initial comparison of four protocol

Diseases of Aquatic Organisms » Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. I. Initial comparison of four protocol | Aquaculture Directory | Scoop.it

Eight laboratories worked collectively to evaluate 4 real-time RT-PCR (rRT-PCR) protocols targeting viral hemorrhagic septicemia virus (VHSV) being considered for deployment to a USA laboratory testing network. The protocols utilized previously published primers and probe sets developed for detection and surveillance of VHSV. All participating laboratories received and followed a standard operating protocol for extraction and for each of the rRT-PCR assays. Performance measures specifically evaluated included limit of detection (defined as the smallest amount of analyte in which 95% of the samples are classified as positive), analytical specificity, assay efficiency across genotype representatives, within- and between-plate variation within a laboratory, and variation between laboratories using the same platform, between platforms, and between software versions. This evaluation clearly demonstrated that the TaqMan®-based assay developed by Jonstrup et al. (2013; J Fish Dis 36:9-23) produced the most consistent analytical performance characteristics for detecting all genotypes of VHSV across the 8 participating laboratories.

 


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Method for detection of drug resistance in sea lice.

Method for detection of drug resistance in sea lice. | Aquaculture Directory | Scoop.it
Just as they have a method for detecting drug resistance in bacteria against antibiotics, researchers have found a way of doing the same for this parasite, the sea louse, that plagues the salmon industry in the north.

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John Bostock's curator insight, September 11, 2013 9:32 AM

This could help to avoid ineffective and costly treatments

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A two tube, nested PCR detection method for AHPND bacteria - News

A two tube, nested PCR detection method for AHPND bacteria - News | Aquaculture Directory | Scoop.it

A new method for the detection of AHPND bacteria AP4 has been published. The advantage of the AP4 method over the previously published AP3 method is that the it has 100 times higher sensitivity. Because of its higher sensitivity, the bacterial culture enrichment step needed when using the AP3 with low levels of AHPND bacteria may be omitted. However, the AP4 method should not be considered as a replacement for the AP3 method but simply as an alternative choice for the users to choose should they need a more sensitive detection method.

 

The AP4 PCR method was developed entirely by Thai scientists working in Thailand at Centex Shrimp,the Shrimp-virus interaction laboratory, BIOTEC and Aquatic Animal Health Research Center and Charoen Pokphand Co. Ltd. It was also supported entirely by research funding from Thailand. 


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John Bostock's curator insight, March 10, 2015 6:13 AM

Sorry - a little late spotting and publishing this.

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A new species of the fish pathogenic bacterium Edwardsiella - NVH

A new species of the fish pathogenic bacterium Edwardsiella - NVH | Aquaculture Directory | Scoop.it

Takele Abayneh Tefera's doctoral research project has uncovered a genetic divergence between the fish pathogen Edwardsiella tarda and Edwardsiella tarda type strain. He has also identified phenotypic markers that distinguish one from the other. The fish pathogenic strain is now classified as a separate species: Edwardsiella piscicida.

 

 

Edwardsiella tarda is a bacterium that can infect a number of animal species and also humans. Edwardsiellosis is one of the most serious systemic bacterial diseases in fish, resulting in substantial losses in the fish farming industry all over the world.

 

Takele Abayneh Tefera has developed effective molecular tools for the identification and characterization of different strains of Edwardsiella. He developed a new TaqMan real-time and conventional PCR analysis for this purpose and then evaluated it in relation to the Loop Mediated Isothermal Amplification (LAMP) analysis. For the first time, he also used the Multi-locus Sequence Analysis (MLSA) for the typing and characterization of E. tarda, isolated from different sources. This is a useful tool for detecting sources of infection and for understanding the epidemiological relationship between isolates from the environment, fish, livestock and humans.


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