The authors used proteomics to explore the underlying mechanisms of ALS, and to find out the biomarker for early diagnosis and therapies.
Objective: To investigate the difference of protein expression and modification in different area of the nervous system. To address the pathophysiological mechanisms underlying ALS development.
* No significant difference of protein expression except spinal cord. These proteins are modified with different charges.
* The most significant difference is GFAP, which is up-regulated in spinal cord of ALS. Also some others are different in protein expression, such as: NFL, Vimentin and so on. NFL is decreased, Vementin is up-regulated. In addition, some of them were degraded into fragments.
* By using the antibody against common acetylated protein, we find protein acetylation is different in ALS and NON-ALS. Using LC-MS/MS, we first identified three acetylated proteins: Tublin, GFAP and UBS3A.